查询词典 ELISA

Methods the plasmic levels of pai-1 and t-pa in diabetic patients with or without diabetic nephropathy and normal controls were tested by elisa.

选择133例t2dm患者，根据尿蛋白排泄率分为无dn组67例、dn微量白蛋白尿组51例、dn临床蛋白尿组15例，正常对照组27例。

The plasma and urine TGF-β1 levels in postsurgery decreased comparing with presurgery, but the cirrhosis group and normal control group have no this phenomenons.

应用ELISA方法检测血和尿中的TGF-β1值，并对照各临床资料及病理标本应用SPSS 11.0行单因素方差分析、直线相关和x〓检验等统计学处理。

Methods M. penetrans was cultured and its LAMPs were extracted. Mouse macrophages were stimulated with M. penetrans LAMPs to analyze the production of nitric oxide. Cell prolification was detected in M. penetrans LAMPs-stimulated mouse macrophages by MTT assay.

用SP-4培养基培养穿透支原体并提取其脂质相关膜蛋白，用提取的脂质相关膜蛋白刺激小鼠巨噬细胞，以ELISA法检测NO的产生，并用MTT法检测其对小鼠巨噬细胞增殖的影响。

Methods: VEGF serum level in 80 cases of severe hepatitis was quantitively detected by using Two-antibody-sandwich ELISA..

采用双抗体夹心法定量检测80例重型肝炎患者血清VEGF水平。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

In order to decide the safety of the virus to human,The susceptibility of Budded Virusto cell strains and mice,occuluded virusin the sheets of liver and renes was observed with microscope and electronic microscope.

为确定家蚕基因工程表达系统产品对人的安全性，观察了该病毒对哺乳动物细胞株及对小鼠的易感性，建立了检测Bm-NPV蛋白组份的dot-ELISA方法，可用于生物表达器生产的产品中残存多角体病毒组份的检测。

Methods 24 SD rats were randomly divided into normal group,model group,aminophylline treatment group(35 mg/kg), 8 rats in each group. The bronchial asthma model in rats was built by egg protein sensibilization and longterm inhalation provocation. The rats in treatment group were lavaged each day from the first time of provocation.After 4week treatment, the rats were killed to obtain lung tissue for staining with HE.

24只SD大鼠随机分为正常组、模型组、治疗组，每组8只，除正常组外以卵蛋白致敏并吸入激发法制备大鼠哮喘模型，治疗组、模型组从第1次哮喘激发开始(造模第3周)分别给予氨茶碱、0.9%氯化钠溶液灌胃给药，1次/d，用药4周处死大鼠，取肺组织HE染色，用彩色图像分析仪测量支气管壁面积、支气管平滑肌面积，用ELISA双抗体夹心法测定肺组织MMP9和TIMP1含量。

HBs with ELISA and Wstern blot in 58 seral samples.

HBs。结果：嵌和蛋白可与抗？

Serologic HBV markers of pregnant women and newborns were detected by ELISA and PCR.

孕妇和新生儿血清HBsAg 和HBV DNA检测分别采用酶联免疫吸附试验和聚合酶链反应法。

The serumal CRP wae determined with Immunonephelometric method, the IL-6 wae determined with double antibody sandwich ELISA method.

比较每组内治疗前后血清中的CRP和IL-6的变化，及两组间试验终点时间血清中的CRP和IL-6变化。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。