查询词典 ELISA

Objective To express and characterize the C terminal fragment of HEV ORF2. Methods The gene encoding C terminal 128 amino acid fragment of HEV ORF2 was inserted into nonfusion expression vector pBV220. The resultant pBVORF2.3 was transformed into E.coli strain DH5α.

用pBV220载体进行抗原的非融合表达，包涵体经变性凝胶过滤层析及离子交换层析纯化后透析复性，以Western blot、ELISA、动物免疫与抗原捕获RT-nPCR等方法研究其免疫学特性。

Methods TORCH-IgM,TNF,IL-6 in 56 cases of recurrent spontaneous abortion, 20 cases of early pregnant and 20 cases of nonpregnant women were determind by ELISA.

方法采用酶联免疫吸附法检测56例流产患者、20例早孕妇女及20例非孕妇女血清中TORCH-IgM、TNF、IL-6的水平。

They were cultured for 48 hours,and the a mount of osteolytic factors such as TNF-a and IL-6 of each group was determined.

培养48h后，用ELISA测定细胞上清液中肿瘤坏死因子-a、白细胞介素-6含量。

The recombinant protein was purified by Ni-NTA affinity chromatography and gel filtration chromatography. The inbred C57BL/6 strains and outbred KM strains of mice were subcutaneously immunized with the protein formulated by either adjuvant ISA720 or aluminium hydroxide.

表达产物通过Ni-NTA亲和层析和凝胶过滤层析方法纯化，再分别与ISA720和Al3佐剂乳化后，免疫C57BL/6和昆明小鼠，通过尾蚴膜反应和ELISA反应检测特异性抗体。

The results showed that the 56kD and 26kD antigens had better immunogenicity and could not react with the sheep positive serum of Mycoplasma pneumonia and negative serum of E. ovis. Of the 64 samples detected, the positive rate was 66.7%(42/64), 22.9% higher than that of direct microscopic examinerion.

结果表明，56kDa和26kDa的羊附红细胞体抗原具有较好的免疫性，而且不与羊附红细胞体阴性血清和羊支原体病阳性血清发生交叉反应，具有较好的特异性；ELISA检测的64份血清样本的阳性率为66.7%，比涂片镜检高22.9个百分点。

The successful transfection of perifollicular cells was quantified by elisa.

制备得到的质粒脂质体制剂能够将质粒转染进皮肤细胞并介导体内瞬时表达。

Methods Ddimer levels were determined by enzymelinked immunoabsorbent assay in patient with suspicion of pulmonary embolism,and carries on for half year to those patients to make a followup visit to see whether they have the symptom of pulmonary embolism or deep phlebothrombosis.

摘 要］目的：探讨D二聚体在排除肺栓塞诊断中的临床价值。方法：使用ELISA法对可疑肺栓塞患者血浆中纤维蛋白降解产物DD进行检测，并对这些可疑肺栓塞患者进行半年的随访，了解是否有肺栓塞或深静脉栓塞的症状。

3Monitoring IL-1β、TNF-a phlegmasia cell factor of CFA postimmune ratss blood with radio-immunity ,and analyze it.

4CFA免疫后用ELISA法检测血中的IFN-r，IL-4水平，并将其比值与Th1／Th2进行相关分析。

Methods: 63 patients with ACVD and 26 age matched healthy volunteers were enroued. Plasmatic levels of TF and TFPI were examined with ELISA technique.

选择脑血管病急性期患者63例和健康体检者26例；采用双夹心酶联免疫吸附抗原测定法测定患者血浆中TF和TFPI的含量，与正常对照组及组间进行比较。

Methods The Plasmic levels of PAI-1 and t-PA in diabetic patients with or without diabetic nephropathy and normal controls were tested by ELISA.

糖尿病肾病(Diabetic nephropathy，DN)是糖尿病的常见并发症，也是糖尿病死亡的主要原因之一。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新