查询词典 ELISA

Some field samples of Solanaceae collected from Zhejiang and Shanxi Provinces were detected by DAS-ELISA and biological tests, results showed that ToMV was a common pathogen of tomato and eggplant. About 50%of detected Solanum melongena samples had ToMV both in Zhejiang and Shanxi provinces.However,there was a significant difference between tomato samples from Zhejiang and Shanxi provinces.In Zhejiang province, only about 10%of samples were infected by ToMV, while the numbers of infected samples were above 60%in Shanxi.

利用此检测系统并结合生物学方法，对浙江及山西等地的田间病样进行测定，结果发现茄子、番茄等茄科作物中均有ToMV的侵染，其中茄子中有50%左右的发生率，番茄中ToMV发生率南北差异较大，在浙江发生率仅为10%左右，而在山西有60.7%的发生率，辣椒中未测到ToMV。

And 86. 90%( 73/84 ) respectively. Conclusion Detection of mumps virus IgM in CSF is one of the useful methods for diagnosis of mumps meningocephalitis.

结论采用ELISA方法检测脑脊液腮腺炎病毒特异性IgM抗体是早期诊断腮腺炎脑膜脑炎的有效方法之一。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估：手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量；应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平；以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜；以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

The optimum work concentration of the indirect ELISA for detecting and screening positive hybridoma cells was established by research again and again. Polystyrene microtitration plates were coated with the purified DHV antigen at the amount of 0.5μg/well.

对鸡胚和鸭胚增殖的尿囊液病毒用反复冻融、氯仿处理、0.22μm滤膜过滤、PEG反透析浓缩以及Sephadex G-200分子筛层析的方法进行纯化，并以此来包被酶标板以及进行BALB/c小鼠免疫，效果良好。

The sandwich ELISA method can be used for detection of serum CMLC with good sensitivity and reproducibility, and is a good technique for the diagnosis of acute myocardiac infarction.

此方法可以检测出人血清中CMLC的含量，重复性好，灵敏度高，是临床诊断心肌梗塞的良好方法。

Cytokine-producing CD4＋ T cells were quantified by 3-color flow cytometry after bring stimulated with phorbol myristate acetate and ionomyccin. After being stimulated with PHA, the levels of IFN-γ and IL-4 in culture supernatants were measured by ELISA.

同时分离外周血单核细胞，经植物血凝素刺激培养后，酶联免疫吸附方法检测培养上清液中IFN-γ和IL-4水平。

Cytokine-producing CD4＋ T cells were quantified by 3-color flow cytometry after bring stimulated with phorbol myristate acetate and ionomyccin. After being stimulated with PHA, the levels of IFN-γ and IL-4 in culture supernatants were measured by ELISA.

同时分离外周血单核细胞，经植物血凝素刺激培养后，应用酶联免疫吸附方法检测培养上清液中IFN-γ和IL-4水平。

Results: In this method,antigen and antibody reacted on the membrane by filtration and the result could be observed with nake...

结果 ：抗原抗体通过渗滤在膜上反应，10min肉眼即可观察到结果，10 0例临床血清标本的检测结果，本法与ELISA法基本相符。

Methods 84 nasopyaryngeal carcinoma patients,57 nasitis patients and 60 healthy control were selected. EBVCA-IgA and EBNA-IgA in serum were determined to evaluated the diagnostic value of the combined determination of the two indexes.

分别采用免疫酶法和ELISA间接法检测60例正常人，57例鼻炎患者和84例鼻咽癌患者血清中EB病毒壳抗原IgA和EB病毒核抗原IgA，评价两项指标联合检测对鼻咽癌的诊断价值。

The acute virus necrobiotic disease virusC AVND viruswas isolated from diseased scallop Chlamys farreri collected in the Taiping Bay of Qingdao. Using purified virus as antigens, the polyclonal antibody with high titer was prepared in rabbits. The polyclonal antibody as the first antibody, IgG-HRP as the second antibody, indirect ELISA technique was set up through selecting the best interactional conditions among the first antibody, the second antibody and antigen.

以患病栉孔扇贝组织中提纯的急性病毒性坏死症病毒作为免疫抗原，注射新西兰白兔制备出效价高达1：32000的多克隆抗体作为第一抗体，第二抗体为辣根过氧化物酶标记的羊抗兔IgG，通过交叉稀释法确定抗原、一抗及二抗的最优反应条件，建立酶联免疫吸附分析方法体系，病毒检测灵敏度达30ng。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新