查询词典 ELISA

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

Objective To study the influence of HBsAg results with ELISA of different cleaning the troughs Methods The hemolysis or feculent serum samples is frequent in the ordinary work.

目的 探讨不同洗板方式的酶联免疫法对HBsAg结果的影响。

Methods: The preoperative serum samples of patients with gastroenteric carcinoma and colorectal carcinoma and the serum samples of normal persons were collected.

收集胃癌、结直肠癌患者术前以及正常人血清标本，用ELISA法检测血清VEGF含量，用放射免疫法检测CEA含量。

METHODS: The expression of GATA- 3 gene was analyzed by using RT - PCR - ELISA in BMSCs from 34 normal cases and 9 cases with AA.

目的：了解转录因子GATA-3基因在再生障碍性贫血和正常骨髓基质细胞中表达的情况，探讨AA发病的免疫机制。

Methods In this cross-sectional study, 112 patients with hematencephalon and 82 controls were tested serologically for specific CpIgG antibody with ELISA assay.

112例脑出血患者和82例非脑出血患者，应用酶联免疫吸附试验检测血液中的Cp特异性IgG抗体。

The antibodies of measles,diphtheria and pertussis were detected with ELISA,agglutination and indirect hematocyte agglutination test,respectively.

我区自 1984年实施儿童计划免疫以来取得了显著的成绩，1990年和 1995年相继实现了以县、乡为单位儿童免疫接种率达 85 %的目标。

Objective To investigate changes of vWF in vascular endothelial cells with acute hypoxia and assess its significance in the study of vascular injury and hypercoagulability. Methods vWF in vascular endothelial cells and in blood were measured by immunohistochemical staining image analysis and ELISA technique.

目的 观察急性缺氧时内皮细胞中血管性血友病因子的变化及其与血液高凝状态之间的关系，确定其在反映血管内皮细胞损伤和血液高凝状态研究中的价值。

Levels of bFGF and TGFβ1 in the rabbit ear hyperplastic scar were determined by enzyme-linked immunosorbant assay..

通过ELISA的方法分别检测用药后1天、3天、7天、14天、21天、30天和40天兔耳增生性瘢痕中的bFGF和TGFβ1表达水平。

Methods PI 1 anti idiotype monoclonal antibodies against pemphigus and purified PAb from the serum of a patient with active pemphigus were used to establish an ELISA system for detecting the PAb.

目前常用的PAb检测法为免疫荧光法和免疫印迹法等，都需要一定的实验条件，难以在临床推广使用。

The expressed His-fusion protein was shown to be immunologically active by Western blotting.

经ELISA和Western Blotting检测，表达的重组蛋白具有免疫学活性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新