查询词典 ELISA

These results indicated that the BP26 protein can be used as the coating antigen in indirect ELISA test for the serological diagnosis of B. abortus infection in cattle.

abortus天然缺失OMP31基因），BP26作为间接ELISA包被抗原用于牛布氏杆菌血清学检测具有良好的特异性及较好的敏感性。

By using the above established ELISA,we Examined 35 cases of HSV-1 infected serum and 43 cases of negative serum.The results showed 91.4%of sensitivity,97.6%of specificity,97.0%of positive predictive value,93.3%negative predictive value,accuracy rating 94.5%.

以上述条件建立的ELISA对35份HSV-1感染的阳性血清和43份阴性血清进行检测，其敏感度为91.4%；特异度为97.6%；阳性预测值为96.9%；阴性预测值为93.3%；准确度为94.8%。

Methods Plasma IL-8 levels in 30 ACD patients(patients with acute 40 cerebral hemorrhage, 32 patients with acute cerebral infarction)and in 30 healthy subjects were measured by ELISA.

采用ELISA方法测定了74例急性脑血管病患者(40例脑出血，34例脑梗死)和30例健康对照者的血清IL-8含量。

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

Papaya leaf curl samples collected from Guangxi province and 1 from Guangdong province, and 5 ageratum yellow vein samples from Guangxi province were tested with triple antibody sandwich ELISA and PCR, Results showed all the virus samples were infected by whitefly-transmitted geminiviruses.

利用三抗体夹心ELISA及PCR检测的方法对采自广西的7个番木瓜曲叶病样品和广东1个番木瓜曲叶病样品，及采自广西胜红蓟的5个黄脉样品进行了检测，表明均为粉虱传双生病毒。

Geminiviruses infecting Ageratum conyzoides and Stachytarpheta jamaicensis were identified with molecular data in this paper.5 virus samples collected from Ageratum conyzoides and Stachytarpheta jamaicensis in Hainan were detected with triple antibody sandwich ELISA and PCR, results showed all the virus samples were infected by whitefly-transmitted geminiviruses.

本研究对侵染胜红蓟和假马鞭的双生病毒进行了分子鉴定，并获得如下结果：利用三抗体夹心ELISA及PCR检测的方法对采自海南的胜红蓟和假马鞭的5个病样进行了检测，表明均为粉虱传双生病毒。

The levels of schistosome circulating cathodic antigen in serum and urine were detected parallelly by McAb-Dot-ELISA. In 81 acute cases, the positive rates in serum and urine were 96.30% and 66.67%(P＜0.01) but the combined positive (positive in serum and／or in urine) rate reached 100%, while in 109 chronic cases, they were 77.06% and 10.09%(P＜0.01) but the combined positive rate was not increased. In 100 healthy individuals from non-endemic areas, no positives were detected in serum and urine with false positive rate of 0%. The cross reaction rates in serum and urine of 51 cases with clonorchiasis were 9.43% and 0% but all were 0% in 48 cases with ancylostomiasis.

提要 应用McAb-Dot-ELISA平行检测血吸虫病人的血清和尿样(浓缩20倍)中日本血吸虫肠相关趋阴极循环抗原。81例急性血吸虫病患者的血清和尿样的阳性率分别为96.30%和66.67%，而血清和尿样组合检测的阳性检出率提高至100%。109例慢性血吸虫病患者的血清和尿样的阳性检出率分别为77.06%和10.09%，组合检测的阳性检出率没有提高。100份健康人的血清和尿样的假阳性率均为0%。54例华支睾吸虫病患者的血清和尿样的交叉反应率分别为9.43%和0%。48例钩虫病患者的血清和尿样的交叉反应率均为0%。

ELISA and Western blotting showed that the recombinant protein has good antigenic.

ELISA和Western blotting实验证实，重组蛋白具有良好的抗原性。

We collected Leishmania promastigotes,washed its with 0.01mol/LpH7.2 buffers ,smashed its,and then measured protein content and titrated work concentration.2.We tested Kala-azar patients"serum and healthy persons"serum of epidemic area by ELISA. The results of three antigensmethods were the same, work concentration of serum was the same(l :100).Results:The protein content of three antigens is, 901 strain with 1000ug/ml,801 strain with 960ug/ml and 951 strain with 1100ug/ml. Suitable work concentration is, 901 strain with 10ug/ml ,801 strain with 12ug/ml and 951 strain with 11ug/ml.

1、抗原的提取：收集新疆不同地区分离得到的利什曼原虫前鞭毛体，经培养，用0.01mol/L pH7.2的磷酸盐缓冲液漂洗，超声粉碎，比色法测定其蛋白质含量，间接ELISA法滴定出抗原的工作稀释度。2、检测：用提取的三株抗原，分别进行间接ELISA法测定黑热病病人血清和疫区健康人血清抗体，并以肺结核病人血清、非疫区健康人血清做对照，三株抗原所用检测方法一致，血清工作稀释度均为1∶100。

A specific peptide according to FMDV NSPs B-cell epitopes was synthesized by a solid-phase method, and was conjugated with carrier protein BSA. An ELISA for detecting FMDV NSPs antibody was developed by using the conjugating protein as the coating antigen.

固相法合成特异性FMDV NSP B细胞表位肽，将其偶联在BSA载体蛋白上，作为抗原包被在ELISA板上，制备检测抗FMDV NSP抗体的ELISA试剂盒，并对该试剂盒进行方法考核。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。