查询词典 ELISA

Methods Density gradient centrifugalization and absorption technique were respectively applied to detach peripheral blood mononuclear cells and peripheral blood lymphocytes.

密度梯度离心及吸附法分离外周血单个核细胞和外周血淋巴细胞，采用抗CD4和CD8抗体分别制备CD~(8+)和CD~(4+)T细胞进行培养，应用MTT比色法及ELISA法分别检测T细胞增殖活性及培养上清液IL-2水平。

Methods By using to turn differently to carry on centrifugence to the same batch of specimen with time soon,and then sing ELISA examination HBsAg.

检测乙肝表面抗原，常采用酶联免疫吸附试验夹心法以及胶体金免疫层析法。

The highest titer of anti-CT mAb (CT-3A and CT-9A) determined by indirect ELISA was 1:15,625. Secondly, we made the colloidal gold from the chloroauric acid (HAuCl4) and labeled anti-CT mAb with the 20 nm colloidal gold.

第二部分进行胶体金合成工作，并成将霍乱毒素单株抗体与20 nm胶体金进行抗体染色，进一步应用於霍乱毒素免疫分析试片之开发上。

A new wheat virus disease was reported in Jiangsu province, China. Wheat plants infected showed chlorotic or yellow stripes which was continuous or discontinuous on the leaves, the diseased new leaf became yellow or seared and the spike was unseeded.

为了确诊在江苏小麦上新发生的一种病毒病害，采用ELISA、RT-PCR和序列测定等方法对其病原进行了精确鉴定，并对此病的田间发生流行规律进行了调查分析。

We also study the human umbilical vein endothelial cells, whose proliferation rate, integrity and endocrinein function on the effect of cholic acids, to find how its injury on HUVEC to cause the maternal and fetal complication of ICP.

此外，本研究还利用原代培养的人脐静脉内皮细胞，通过MTT和ELISA方法探讨胆酸对内皮系统增殖及其完整性和内分泌功能的影响，从而明确胆酸对内皮系Pane统的损伤在ICP胎儿并发症发病机制中的作用。

This method has been successfully applied to the detection of human chorionic gonagotropin.

利用此方法成功应用于检测人绒毛膜促性腺激素，灵敏度高于经典ELISA法。

Circulating antigen was detected in 29 out of 70 cases with paragonimiasis with a sensitivity of 41.5%. The rate of cross reaction in cases with clonorchiasis sinensis and schistosomiasis was 25%（5/20） and 20%（4/20）, respectively, and it was negative in 60 casess with other parasitic infections and healthy subjects, with an overall specificity of 93.6%.

用CAg-dot-ELISA检测70份卫氏并殖吸虫病临床诊断患者血清，阳性29份，敏感性为41.5%（29/70），与华支睾吸虫病和日本血吸虫病患者血清分别有25%（5/20）和20%（4/20）的交叉反应，与其他寄生虫感染者血清和健康人血清（60份）均为阴性，特异性为93.6%。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Objective To study the antigenpresenting function of splenic dendritic cells in mice after hemorrhage plus closed fracture.Methods Splenic DCs were isolated 24h after hemorrhage plus closed fracture,then the ability to stimulate nonadherent splenocytes proliferation in the presence of conalbumin in vitro was detected,IL12 and IL10 levels in cell supernatants were measured by ELISA kits.

大量研究表明，创伤能引起机体免疫系统产生复杂的变化，树突状细胞(dendritic cells，DC)是体内功能最强的专职抗原递呈细胞(antigenpresenting cells，APC)，它可摄取和加工抗原，并分泌多种细胞因子，在激发T 细胞的免疫应答中起重要作用。

Objective To study the antigenpresenting function of splenic dendritic cells in mice after hemorrhage plus closed fracture.Methods Splenic DCs were isolated 24h after hemorrhage plus closed fracture,then the ability to stimulate nonadherent splenocytes proliferation in the presence of conalbumin in vitro was detected,IL12 and IL10 levels in cell supernatants were measured by ELISA kits.

大量切磋证明，创伤能引起机体免疫编制发生庞杂的改变，树突状细胞(dendritic cells，DC)是体内效用最强的专职抗原递呈细胞(antigenpresenting cells，APC)，它可摄取和加工抗原，并渗透多种细胞因子，在激励T 细胞的免疫应答中起重要作用。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新