查询词典 ELISA

The Interference of cell cycle and proliferation of BGC-803 and Hela cell by water extraction of Agrimonia is studied by electronic microscopy and flowcytometry respectively, and the IL-12 and TGF-β are also detected by ELISA in order to explore the pharmacological mechanism of Agrimonia.

仙鹤草抗肿瘤研究应用流式细胞技术和电镜技术，在体外分别研究了仙鹤草水提取液对胃癌BGC-803和宫痉癌Hela细胞的生长抑制作用和细胞周期的影响，同时检测对免疫活性因子TGF-β和IL-12表达的影响，阐明其对人癌细胞生长抑制的药理机制。

Various kinds of allergenic detection in the serum of 329 patients with allergic diseases. Methods To use the method of ELISA to detect

目的：通过研究329 例过敏性皮肤病患者血清中各类过敏原的捡出率，了解各种过敏原在洛阳地区过敏性皮肤病

The patient avoided eating food rich of protein for three days and fasting for 12 hours before the blood samples were obtained at 7:00-8:00 in the morning, and had laid on bed for 15 minutes at least, 2ml of the blood samples were taken from median antebrachial vein, put into the heparinized tubles, then centrifugating by 1500/minute for 10 minutes,obtained the plasma from the blood, the plasma sample were kept in -72℃ refrigerator, ELISA method was used to determine the homcysteine level.

患者在标本采集前3天避免食用高蛋白饮食，空腹12小时以上，清晨7：00－8：00采血，采血时平卧位，休息15分钟以上，采集肘正中静脉血2ml，置于肝素抗凝管，以1500转／分离心10分钟后，分离血浆，用酶联免疫吸附法测定各管的HCY含量。

In present study, zooblooting, ELISA and whole-mount immunocytochemistry methods were used to identify the FXPRLamide family neuropeptides from the Japanese oak silkworm, Antheraea yamamai.

利用Zooblotting、酶联免疫吸附测定和免疫组织化学等方法，对天蚕的DH、PBAN等FXPRLamide家族神经肽进行鉴定。

In order to investigate new methods to the detection of Mycobacterium tuberculosis and diagnostic value of FQ-PCR in the detection of DNA in Mycobacterium tuberculosis, FQ-PCR was used to detect DNA of Mycobacterium tuberculosis in body fluid from 68 patients with tuberculosis and 68 patients without tuberculosis and it was also compared with the two methods of smear acid-fast stainning and antituberculotic antibody.

为探讨结核分枝杆菌检测的新途径及其FQ-PCR技术在结核分枝杆菌DNA诊断中的实用价值，运用FQ-PCR技术对68例结核病患者和68例非结核病患者体液进行结核分枝杆菌DNA测定，并与涂片抗酸染色及ELISA—抗结核抗体法进行比较。

It indicates that not only had FQ-PCR the characteristics of higher positive and accurative rates than smear acid-fast staining and antituberculotic antibody, but also had the advantage of accuracy quantitative analysis. It is of greater applicable importance that early diagnostic and medical treatment depend on FQ-PCR etiology data.

认为FQ-PCR技术不但具有明显高于涂片抗酸染色及ELISA—抗结核抗体法的阳性率和准确度，而且还具备准确定量分析的优点，为病人早期诊断与药物疗效观察提供了更为精确的病原学依据，具有更大的实用价值。

Three different antiserum, rabbitantiserum against formalin-killed-cells of V. parahaemolyticus zj2003 (apathogenic strain isolated from diseased large yellow croaker, from Xiangshan bay,Zhejiang province), rabbit antiserum against OMP of the bacteria and large yellowcroaker convalescent antiserum were prepared for western blotting of the OMPpreparation.

以饱和硫酸铵梯度盐析法结合琼脂糖柱sephadex G200凝胶过滤法初步纯化了大黄鱼血清免疫球蛋白，再经割胶回收法得到进一步纯化的重链片段，分子量约为75kDa，免疫新西兰兔后获得兔抗大黄鱼免疫球蛋白血清，为ELISA和Western blotting检测技术奠定了基础。

Methods The level of serum and urinary tissue-type plasminogen activator and plasminogen activator inhibitor-1 (PAT-1) were measured in 38 patients with chronic glomerulonephritis, 28 patients with nephropathy syndrome, 36 patients with chronic renal failure and 20 healthy subjects by means of enzyme-linked immunosorbent assary. The relationship among urinary t-PA, PAI-l and serum t-PA, PAT-1, urinary protein, serum creatinine were also observed. Results Levels of serum t-PA, PAI-1 in chronic kidney diseases increased remarkably compared with control group.

选择38例慢性肾小球肾炎，28例肾病综合征，36例非透析治疗的慢性肾功能不全和20例正常对照作为研究对象，应用ELISA法检测血清和尿液中组织型纤溶酶原激活剂和纤溶酶原激活物抑制剂－1(PAI-1)的浓度，同时分析尿中t-PA和PAI-1的水平与血t-PA、PAI-1、血肌酐和24h尿蛋白总量之间相关性。

Methods Rat renal I/R model was performed by placing the atraumatic microvascular clamp in both renal artery for 60 minutes.

方法建立大鼠肾脏IRI模型，采用ELISA法检测IRI术后不同时间段血、尿VEGF水平，并与肾功能做相关分析。

At the same time, intracellular DNA was extracted and subjected to Southern blot, hybridized with a ~(32)P-labeled HBV probe and autoradiographed. The intensity of the autoradiographic bands was quantitated by densitometric scans of computer and ED_(50) was calculated.

然后以2.2.15细胞培养为基础，用不同浓度的β-L-D4A进行干预后，经ELISA检测上清液中HBsAg及HBeAg的含量，提取病毒DNA后进行Southern印迹、~(32)P标记HBV探针杂交、放射自显影，经计算机图像处理进行定量分析，求出50％抑制的药物浓度，即EC_(50)。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。