查询词典 ELISA

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

HMGB1, IL-6 levels in plasma and ultrafiltrate were measured by enzyme linked immunosorbent assay.

采用酶联免疫吸附法(enzyme linked immunosorbent assay，ELISA)测定血浆和超滤液HMGB1、IL-6水平。

The concentrations of TNF-α,IL-10 in serum,ultrafiltrate weremeasured 0,1,12,and the end of CVVH by ELISA, the concentration of NO was measured by nitrate reductase method.

CVVH过程中临床指标的观察与检测：生命体征、肾功能、动脉血氧分压及血氧饱和度等。

TNF-α and IL-10 plasma concentrations were measured by ELISA. Results: Plasma concentrations of TNF-α fell significantly from (419.4±77.5) pg/ml to (168.1±55.4) pg/ml after treatment, much lower than that of MODS group (P.05), but we did not find TNF-α in the ultrafiltrate. The start of HVHF was associated with a significant and sustained fall of IL-10, which came to the lowest level in the 6th hour after new filter was used, thereafter remained stable during the observation period.

姑果：HVHF组治疗后血浆TNF-α浓度明显下降，从(419.4±77.5)pg/ml降低到(168.1±55.4)pg/ml，各时间点与MODS组有显著差异(P.05)，但滤液中未检测到TNF-α治疗开始后血浆IL-10明显下降，6h达到最低点，此后维持在稳定状态，滤液中能检测到IL-10，随治疗时间延长其滤过系数降低。

Blood sample was obtained from each of the 7 patients by venipuncture.

用竞争性ELISA方法检测病例组与对照组血清标本中PGE_2的水平。

It convinced that the new established Elisa method is a promising technique for early detection and forecast of cotton verticillium wilt.

本研究说明，该方法可在棉花黄萎病的早期检测和预报上具有重要的应用前景。

The rabbits were killed in 4th,Sth and 12th weeks respectively after feeding to obtain the vulnerary plaques to detect the concentration of high sensitive C-reactive proteinthrough ELISA, matrix metalloproteinase-9 （MMP-9）expression evaluated by immunohistochemistry and the changes of morphology observed by HE staining.

采用酶联免疫吸附法检测血清中超敏C反应蛋白的含量，用免疫组织化学法检测MMP-9表达量，HE染色观察颈动脉粥样硬化斑块结构变化。

As the final results, the harvesting wet cell density——186g/L in the fermentation broth and the ELISA units of preS1 antigenicity in the cell——8 000 after 45h main culture were obtained.

实验获得主培养45h后湿菌体浓度为186g／L，细胞内preS1效价为8 000u。

In order to detect the air pollution caused by acarida mites in some special circumstances dust sampler and air-drifting sampler were employed to detect acarida mites in the air, eight species of acarida mites were separated, which belong to 3 families six genus.

为探讨肺螨病的免疫诊断方法，笔者应用IFAT、IHA、Dot-ELISA检测了肺螨病患者血清螨特异性Ig水平，结果显示上述几种方法均具有很好的特异性和敏感性，对于肺螨病的辅助诊断都具有一定的应用价值。

To explore the clinical reliability and usability of the acridinium ester-labeled CLIP and ELISA in detection of AFP to establish a specific, effective, simple and reliable detecting method in our lab.

［目的］探讨吖啶酯标记的化学发光免疫测定(chemiluminescence immunoassay， CLIA)分析技术与酶联免疫吸附试验一步法两种方法检测甲胎蛋白(Alpha-fetoprotein， AFP)的临床可靠性、适用性。建立本室特异、有效、方便、可靠的试验方法。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。