查询词典 ELISA

Based on the rabbit-anti-PRV IgG and McAb, the double sandwich enzyme-linked immunosorbent assay for detection of Pseudorabies virus was developed.

利用所制备的兔抗PRV IgG和McAb建立了检测PRV的"单克隆抗体双夹心ELISA"。

Two pairs of specific primers were synthesized based on the published sequence of some specific gene in PST.

在实验中设定不同梯度的人工接菌浓度，其中ELISA的检出临界值是1％。

The result of telomerase TRAP-ELISA was available within one day and was handled without radioisotope.

该方法可在当日观察结果，不需放射性同位素。

11 patients with ranula(simple ranula 5 cases,plunging raula 6 cases)were treated by OK-432 intralesional injection,the IL-1αand TNF-βof the cystic fluid were detected by ELISA before and after treatment.

11例舌下腺囊肿患者(单纯型5例，下陷型6例)接受病变内注射OK-432治疗，观察临床疗效，治疗前后抽取囊液分析IL-1α和TNF-β的含量。

The repeatablity in ourPCR-ELISA is satisfactory.

微孔板液相杂交重复性良好。

ELISA showed that antibody responses induced by propolis vaccine were faster than that of oil-emulsion vaccines and aluminum hydroxide vaccines;antibody responses induced by oil-emulsion vaccines were longer in duration than that of propolis vaccines and aluminum hydroxide vaccines;the maximum antibody responses induced by oil-emulsion vaccines and propolis vaccines were higher than that of aluminum hydroxide vaccines;Antibody levels were all significantly boosted by revaccination of these vaccines and in addition to this,oil-emulsion vaccines showed longer antibody duration.

这七种疫苗在抗体产生速度方面以蜂胶苗较快，而油剂苗和铝胶苗免疫后抗体上升速度较慢；在抗体水平的维持能力方面，油剂苗较蜂胶苗和铝胶苗更强；在产生最高抗体水平方面，油剂苗和蜂胶苗能力相当且较铝胶苗为高；在加强免疫的效果方面，这些疫苗二次免疫所产生的抗体水平均较一次免疫更高，蜂胶苗和铝胶苗在第93天时的二免抗体水平已降至同一免相当，但此时油剂苗有着较一免更高的二免抗体水平。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

Papaya ring spot virus was tested with electron microscopy and ELISA from 5 diseased pumpkin samples collected in Yunnan Province.

利用电镜和酶联免疫法在云南省采集到的5份南瓜病样中检测到番木瓜环斑病毒(Papaya ring spot virus，PRSV)。

Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

通过实验优化检测条件，最佳捕获抗体工作浓度为4μg/mL、最佳检测抗体工作浓度为1：25倍稀释、最佳反应时间为2h；与马铃薯Y病毒、莴苣花叶病毒、番茄环斑病毒等均未出现交叉反应；阳性样品经64倍稀释后依然可检出，检测灵敏度是传统微孔板ELISA的4倍。

With the use of IgY-based ELISA method, we found that 15%,45%,90% and 100% of the slightly infected mice (5 cercarie per mouse) turned to be negative by detection of circulating SEA when treated by Praziquantel at 2, 4, 6 and 12 months postinfection, respectively; For the moderately infected mice (15 cercarie per mouse), SEA was undetectable in 5%,25%,40% and 70% of mice respectively after treatment at 2, 4, 6 and 12-months postinfection; With the same treatment, 0%,10%,30% and 60% of the heavily infected mice (35 cercarie per mouse) turned negative of SEA detection.

轻度感染(感染尾蚴5条/只小鼠)治疗后2个月，4个月，6个月，12个月的转阴率为15%，45%，90%，100%；中度感染组(感染尾蚴15条/只小鼠)治疗后2个月， 4个月， 6个月，12个月的转阴率分别为5%，25%，40%，70%；重度感染组(感染尾蚴35条/只小鼠)治疗后2个月， 4个月， 6个月，12个月的转阴率分别为0%，10%，30%，60%。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新