查询词典 ELISA

A sandwich ELISA was established taking the antiserum as the primary antibody. The sensitivity of the ELISA was 0.412 ng GH/ml of sample. Inter-assay and intro-assay coefficients of variation were 2. 58％(n=6) and 5. 69％(n=6) respectively. Serial dilutions of serum and pituitary homogenate from orange-spotted grouper yielded dose response curves that were parallel to the standard curve. And the GH of Acanthopagrus butcheri was also parallel to the standard curve. Prolactin of goldfish and recombinant growth hormone of common carp showed no cross-reactivity with the antiserum of orange-spotted grouper GH. It showed that the established RIA was specific and sensitive.

以斜带石斑鱼重组GH抗血清为第一抗体，羊抗兔抗体为第二抗体建立了双抗体夹心酶联免疫测定法，该系统的灵敏度为0.412ng GH／ml(n=6)，组内变异系数为2.58％(n=6)，组间变异系数为5.69％(n=6)，斜带石斑鱼血清和脑垂体稀释液曲线与斜带石斑鱼重组GH蛋白的标准曲线相平行、黑棘鲷GH的系列稀释曲线与标准曲线相平行，金鱼催乳素、重组鲤鱼GH的系列稀释曲线与标准曲线不平行，表明建立的斜带石斑鱼GH ELISA具有良好的特异性和重复性，灵敏度达到了测试血清样品中GH的水平。

We have summed up the immunodiagnosis results of 507 confirmed taeniasis and cysticercosis patients.The special circulating antibody positive rates were 67.5%, 63.8% in sera, and 59.4%,46.1% in cerebrospinal fluidby IHA and ELISA respectively.

本文总结507例临床确诊的绦、囊虫病人血清和脑脊液免疫学测定结果，分别以IHA、ELISA测定循环抗体，以夹心ELISA测定循环抗原，其阳性检出率依次为血清67.5％、63.8％、60.6％；脑脊液为59.4％、46.1％、67.5％。

Antibody against Swine Fever Virus was detected by PPA-ELISA on the microtitration plates coated with purified SFV antigen, when the sample sera were diluted at 1:80, there was significant correlation between the absorbence atwavelength of 490nmA_(490 and ELISA tiler.

前文〔'〕报道了PPA--ELISA在猪瘟免疫监测上的应用及其质量控制。在生产实践中，对于疫苗接种效果的评价和最佳免疫程序的制订，仅做抗体定性测定是不够的，还应做定里或半定量分析。

The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.

对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明，PCR-ELISA检出22份阳性，比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高，而20份证实无结核分枝杆菌的标本，几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。

Methods ELISA was used to detect the specific IgG and IgG 4 antibodies in sera from the patients with clonorchiasis and other parasitic diseases and from healthy persons. Results The sensitivity and specificity of IgG 4 antibodies detected by ELISA in sera from the patients with clonorchiasis showed no significant difference with that of IgG detected.

用华支睾吸虫成虫可溶性抗原进行ELISA分别检测华支睾吸虫病患者(76例)血清中的特异性IgG和IgG4 抗体及日本血吸虫病、并殖吸虫病、囊尾蚴病患者(分别为 63、3 5、41例)和健康者(65人)血清中的交叉抗体。

Avidin-biotin complex ELISA in detecting the specific antibody in patients infected with Clonorchis sinensis was studied.

生物素一亲和素这一生物反应放大系统引入酶联免疫吸附试验建立的ABC一ELISA方法，经对一些可溶性抗原、抗体检测研究，证明敏感性和呈色反应强度均高于常规ELISA，特别有助于低水平抗原抗体检测〔卜7，。

ELISA method was used for comparison. Results The positive rate of DIGFA in testing the serum from 50 infected rats was 96.0%(48/50). The negative rate of DIGFA in healthy rats was 100%(50/50). Cross reaction with Enteroclysis (13 samples) and Tapeworm (11 sqamples) was not observed. The cross reaction rate in 18 Strongyloidiasis rats was 5.6%. The sensitivity and specificity of DIGFA is comparable with ELISA (96.7%).

结果 用DIGFA检测广州管圆线虫实验感染鼠血清50份，其阳性检出率为96.0%，50份正常鼠血清的阴性符合率达100%，DIGFA分别检测蛲虫阳性鼠血清13份，绦虫阳性鼠血清11份和粪类圆线虫阳性鼠血清18份，前二者均为阴性，后者交叉反应率为5.6%；DIGFA与ELISA平行检测30份广州管圆线虫阳性鼠血清，两法符合率达96.7%。

Objective To study the influence of HBsAg results with ELISA of different cleaning the troughs Methods The hemolysis or feculent serum samples is frequent in the ordinary work.The HBsAg test by ELISA of the samples are often present fake positive.

目的 探讨不同洗板方式的酶联免疫法对HBsAg结果的影响方法在日常工作中，伴有溶血或其它因素引起浑浊的血清样品较常见，而些样品在HBsAg的ELISA检测中常出现假阳性，为消除这些假性结果。

Pallidum-antibody with indirected-ELISA based on the fusion protein. Zealand rabbits were immunized with the fusion protein, antibodies to anti-Gpd in sera were detected with indirected ELISA.

同时用纯化的Gpd重组蛋白免疫新西兰兔，间接ELISA方法检测免疫兔血清中Gpd多克隆抗体的效价，对Gpd重组蛋白的免疫原性进行分析。

ELISA and CsCl- isopycnic ultracentrifugation purification products in each fraction indicated that the particle densities of the fraction with higher rHBcAg antigenicity mainly distributed at the densities of 1.27g·mL-1 and 1.40 g·mL-1, respectively.

分管收集的超离心纯化产物经ELISA抗原活性检测和密度分析，可知ELISA反应强度较高的收集管中的颗粒密度主要分布在1.27g/mL和1.40 g/mL两个峰值处。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。