查询词典 ELISA

Methods: Using the ELISA and RT-PCR to detect Rotavirus and astrovirus respectively in stool of the 117 in-patient children diagnosed of enteritis in 2003. The astrovirus positive specimens tested by RT-PCR firstly were serotyped by RT-PCR secondly using the serotype-specific primers, the epidemiological and clinical data including age, season distribution, chief symptoms were compared and analysed.

分别对2003年收集的我院117例腹泻患儿运用ELISA法检测轮状病毒及用RT-PCR法检测星状病毒，并应用两次扩增法对星状病毒进行分型，并对其临床及流行性学资料进行统计学分析，包括患儿年龄，感染季节及主要临床症状等。

HHV-6 positive rates were 60% by nested PCR assay and 40% by ELISA in the salivas of oral squamous cell carcinoma patients.

口腔癌患者唾液的HHV-6阳性检测率结果分别是：套式PCR法60％，ELISA法40％。

Rabbit was immunized by the conjugated alginate-BSA (1.0mg/kg) for 40-days routine immunity method; ELISA method was used to examine the titration of anti-alginate serum; The first generation parotid acinar cells were chosen to divided five groups (group A :contrast, group B: BAS, group C: alginate, group D: anti-alginate serum, group E: alginate +anti-alginate serum), We examined the proliferation of each part by MTT method at four times (lh, 6h,12h and 24h); In the meanwhile, The growth and shape of parotid acinar cells were observed under phase contrast microscope and scanning electron microscope.16 of the immunized rabbits by alginate were divided equally into Al and Bl groups, 12 normal rabbits were divided equally into A2 and B2 groups as control groups, Each rabbit was affused by 0.3ml(40fil/ml)alginate though parotid duct once time two days.

将藻酸盐与BSA偶连形成具有较强免疫原性的Alginate-BSA交联物免疫兔，按抗原量1.0mg/kg接种于新西兰白兔皮内，间隔10d加强免疫1次，共加强4次后，利用间接ELISA法检测藻酸盐抗体效价：将原代培养的兔腮腺腺细胞按10~4个/ml接种到96孔板上，置入细胞培养箱继续培养3d，分为5组：A空白对照组、B牛血清白蛋白组、C藻酸盐组、D抗藻酸盐血清组和E抗藻酸盐血清+藻酸盐组，分别加入PBS、牛血清白蛋白、藻酸盐或新鲜抗藻酸盐血清继续培养，分别于1h、6h、12h和24h不同的时间点，取出96孔培养板，采用MTT法检测5组腮腺腺细胞增殖情况

And further studied E rosettes experiment and lymphocyte proliferation BrdU-ELISA assay.

并对初步筛选的E玫瑰花环实验和淋巴细胞增殖BrdU-ELISA实验进行了进一步筛选。

Methods: ELISA was used to determine the serum of TPA and sVCAM-1 in pre-eclamptic and normotensive pregnant women, and the correlation of pre-eclampsia and neonatal outcomes was analyzed by rank correlation.

采用酶联免疫吸附法（enzyme linked immunosorbent assay，ELISA）测定子痫前期患者和正常晚孕妇女外周血中TPA、sVCAM-1的浓度，并与子痫前期患者病情及新生儿体重进行相关分析。

Methods:(1) bFGF was loaded with PLGA, PELA and gelatin microspheres. The pharmac characteristics of particle size distribution, loading efficiency, entrapment efficiency, bFGF release kinetics were evaluated with scanning electronic microscope, MTT and ELISA respectively. The prescription with the best pharmac characteristics and the highest bioactivity of bFGF was chosen for the next step.

（1）制备PLGA，PELA和明胶微球，通过扫描电镜和激光粒度分布仪测定其粒径分布；ELISA法测定微球溶出度，包封率，bFGF释出动力学；MTT法测定微球释出的bFGF的生物活性，筛选出一种具有较好药剂学性质和较高bFGF生物活性的微球载体材料。

Although NS4B seems to increase the severity of cell apoptosis, only limited effect was detected by nucleosome ELISA assay and flow cytometry.

不过在nucleosome ELISA assay和flow cytometry的分析上，NS4B对细胞凋亡的影响则不明显。

The results of ELISA display that the expression ofthe Bt crystal protein is different in different tissues and at different developmental stages.

ELISA检测结果表明，Bt杀虫蛋白在抗虫棉中的表达具有时空特异性。

Methods Three hundred and fifty-five clinical serum samples from persons with syphilis or without syphilis were detected by the Toluidine red unheated serum test, the T.pallidum particle agglutination assay and the recombintant antigen-based ELISA,respectively.

的 探讨基因重组抗原ELISA法在梅毒螺旋体抗体检测中的临床意义方法用甲苯胺红不加热血清实验对355份梅毒患者和非梅毒患者的临床血清标本进行检测。

Strains of Ralstonia solanacearum are isolated from 258 samples gathered from 12 counties of Yunnan provinces. The pathogen backward inoculation test and ELISA test show that tobacco lower stem black necrotic in Wenshan and Baoshan prefecture is mostly associated with tobacco bacterial wilt, partial with black shank.

从云南省12个县采集的258个烟草病样中分离青枯雷尔氏菌株219个，病原菌回接试验和ELISA检测结果表明文山州、保山市的烟草茎下部黑色坏死症状病害，大部分为烟草青枯病，伴随部分黑胫病。

By using competitive adsorption of activated carbom to remove unfavorable influence of carbon in carbonaceous gold concentrate on leaching process, the leaching rate of gold concentrate can be improved to 88% as compared with 10%by direct cyanidation.

本文论述了利用活性炭竞争吸附作用，消除含炭金精矿中炭在浸出过程中的影响，使金精矿的浸出率可由直接氰化的10％提高到88％。

The only thing you're ever gonna be king of is King of the Stupids!

你只配做傻瓜的国王！