查询词典 ELISA

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

Methods: Based on B-cell epitope 82~93, a multiple antigenic peptide system was synthesized as immunogen and C-57BL/6 mice were divided into 3 groups randomly. MAP group (n=5) referred to that C-57BL/6 mice were immunised with the immunogen and the polyclonal anti-serum was produced. The polyclonal antibody was identified by using an ELISA assay.

将C-57BL/6小鼠随机分为对照组、MAP组、人参总皂苷组，MAP组在预测MAGE-12的B细胞表位序列为QSDEGSSNEEQE(82-93)的基础上，采用4分枝多重抗原肽结构设计合成抗原肽，免疫C-57BL/6小鼠，产生多克隆抗体，应用ELISA实验检测多克隆抗体是否为人的抗原肽的特异性抗体。

Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原，免疫BALB/c小鼠，按常规方法进行融合，间接ELISA方法进行筛选，采用有限稀释法，经过三次克隆，最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5)，分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类，三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

By means of Cell-ELIsA,FACS,RBA and WB,the Clq receptor on a human B lym- phocyte line, Raji, was characterized.

应用Cell－ELISA、FACS、RBA及WB等技术，对人B细胞系Raji细胞ClqR的特性进行了研究。

Coli, and purify rWHcAg under native condition by precipitation with saturated ammonium sulfate and CsCl gradient centrifugation; Immunize Balb/c mice with purified rWHcAg to prepare antiserum; Establish a competitive inhibition ELISA method to detect WHcAb in serum of Chinese marmots.

原核表达重组WHcAg，氯化铯密度梯度离心获得非变性的纯化蛋白；用该纯化蛋白免疫Balb/c小鼠制备多克隆抗体；建立竞争抑制ELISA方法用于检测旱獭血清中的抗－WHc。

Lipofectamine was used to transfect pVMm into C2C12 and the expression of mMCP-1 was detected by ELISA.

以脂质体将pVMm转染真核细胞系C2C12，ELISA分析mMCP-1的表达情况。

ANCA titres are generally measured using ELISA and indirect immunofluorescence .

抗体滴度通常是衡量用 ELISA法和间接免疫荧光。

The light chain of the McAb was indentified to be κ. Its titres of ascitic fluids were up to 1×10^(-6) by indirect ELISA.

制备单克隆抗体腹水，腹水的间接ELISA效价在1×10^(-6)以上。

Titrations are performed in immuno-fluorescence, ELISA, and western blot so that you may make an educated guess as to the appropriate dilution for your application.

对于免疫荧光，ELISA法和western blot还要进行滴定，让您可以根据您的具体应用进行适当的稀释。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。