查询词典 ELISA

Augmentor of liver regeneration ; Recombinant human augmentor of liver regeneration ; Monoclonal antibody ; Sandwich ELISA ; Histological of ALR

肝再生增强因子；基因重组人ALR的二聚体；单克隆抗体；夹心ELISA ； ALR的组织定位

In the early days avidin extracted from the egg, this egg avidin-alkaline-glycoprotein, and the adsorption of polystyrene carrier highly for ELISA in the background can increase.

在早期，亲和素从蛋清中提取，这种卵亲和素为碱性糖蛋白，与聚苯乙烯载体的吸附性很强，用于ELISA中可使本底增高。

By using the "mechanical wounding"experimental system estabilished by us, and light- and electron microscopy, autoradiography and radioisotope technique, immuno-fluorescence localization, homologous clone and RACE technique, in situ mRNA hybridization and ELISA et al., we investigated the relationship between the transportation of exogenous JA and the secondary laticifer differentiation, the relationship between the producton and accumulation of endogenous JA and the laticifer differentiation from cambia, and the effect of appling exogenous jasmonates on the natural rubber production.

利用我们建立的机械伤害诱导乳管分化的实验系统，采用光学显微镜和电子显微镜技术、同位素示踪和放射自显影技术、免疫荧光定位技术、同源克隆和RACE技术、mRNA原位杂交和ELISA等技术，研究了外源茉莉酸在橡胶树体内的移动及其对维管形成层分化次生乳管的诱导效应、内源茉莉酸与乳管分化的关系以及茉莉酸类物质对天然橡胶产量的影响。

Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心ELISA法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌，在不同时相处死后，观察肠内白念菌总数及肠粘膜表面白念菌粘附数量；取肠系膜淋巴结做白念菌选择培养，观察移位体内发生率；采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况；流式细胞计数潘伊尔氏结细胞表面IgA阳性率；免疫组化染色后计数固有层中IgA浆细胞数量变化；ELISA法测定肠粘液中特异抗白念菌IgA含量。

The monoclonal cells were selected using cloning rings. The expression of capsid protein was detected by indirect immunofluorescence and sandwich-ELISA. The empty capsids of FMDV were observed under electron microscope.

经间接免疫荧光和酶联免疫吸附测定（Enzyme-linked immunosorbent assay，ELISA）方法检测MDBK细胞中衣壳蛋白的表达，并在电镜下观察口蹄疫病毒空衣壳。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

A direct competitive ELISA for carbaryl was established successfully and this method was primarily applied to detect carbaryl in cumcumber samples.

本研究成功建立了甲萘威的直接竞争ELISA法，并将该方法初步应用于黄瓜样品中甲萘威的快速检测。

To develop a double antibody sandwich ELISA for quantitation of Cap43 protein in serum and initially study correlation between concents of Cap43 protein expression in serum from lung cancer patients and healthy adults and tumor histological pattern in lung cancer.

目的建立定量检测血清Cap43蛋白表达双抗体夹心ELISA 法，并初步探讨血清Cap43蛋白表达水平在肺癌患者与正常人群间有无差异，以及该蛋白表达水平的高低与肺癌组织学分型间的关系。

To develop a double antibody sandwich ELISA for quantitation of Cap43 protein in serum and initially study correlation between concents of Cap43 protein expression in serum from lung cancer patients and healthy adults and tumor histological pattern in lung cancer.

目的 建立定量检测血清CaP43蛋白表达双抗体夹心ELISA法，并初步探讨血清Cap43蛋白表达水平在肺癌患者与正常人群间有无差异，以及该蛋白表达水平的高低与肺癌组织学分型间的关系。

By using competitive adsorption of activated carbom to remove unfavorable influence of carbon in carbonaceous gold concentrate on leaching process, the leaching rate of gold concentrate can be improved to 88% as compared with 10%by direct cyanidation.

本文论述了利用活性炭竞争吸附作用，消除含炭金精矿中炭在浸出过程中的影响，使金精矿的浸出率可由直接氰化的10％提高到88％。

The only thing you're ever gonna be king of is King of the Stupids!

你只配做傻瓜的国王！