查询词典 ELISA

Objective To discuss the assay methods and clinical value of IgG antibody to Mycobacterium Tuberculosis. Methods To detect the antibody levels of tuberculoprotein 16KDa, 38KDa and LAM by Protein Chip and ELISA together, the sensitivity, specificity and total coincidence rate of the two methods were compared.

目的 探讨结核分枝杆菌IgG抗体的检测及其临床应用价值方法应用蛋白芯片识别仪分析结核病人血清中结核菌蛋白16Kda和38Kda以及脂阿拉伯甘露糖抗体含量，同时与ELISA法进行比较，判断其敏感性、特异性和总符合率。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

In order to test the antibodies to E6 and E7 induced by the recombinant vaccina virus, the fusion proteins of GST and E6 or E7 were expressed in E. coli using pGEX-2T vector. The expressed proteins were confirmed by Western-blot and purified by glutathione sepharose CL-4B affinity chromatography. The purified proteins were used to detect the anti-E6 and-E7 by ELISA.

为便于检测重组痘苗病毒所诱发的E6和E7抗体，本文利用原核表达载体pGEX-2T，在大肠杆菌中分别高效表达了GST与E6或E7的融合蛋白，并利用纯化的融合蛋白成功地建立了E6和E7抗体的ELISA检测方法。

Owing to the variability of HVR1, the method to check anti-HVR1 antibodies need to cloning and expressing the gene of HVR1 of every sera and that poses some problem. For example, it needs lots of time and money, and the sample must come from viremic patients. It is urgent to acquire a broadly cross-reactivity HVR1 antigen to check the antiHVR1 Ab in the sera of HCV infected patients by ELISA.

由于HVR1的高变性，因此要想测定血清中抗HVR1水平，通常要克隆表达待测血清中的HVR1基因，但该方法时间长、费用高，并仅对HCV、RNA阳性血清适用，所以急需一种具有极高交叉反应性的HVR1抗原，才可通过ELISA对病人的HVR1抗体进行检测。

Indirect ELISA was then used for detection of RSV in Laodelphax striatellus Fallen. The proportion of the viruliferous Laodelphax striatellus in Jiangsu province ranged from 12.5% to 41.5%.

用建立的间接ELISA对江苏省部分县市大田灰飞虱带毒率进行检测，结果显示灰飞虱的带毒率为12.5％-41.5％。

Using indirect ELISA, the contents of vitellin in the fat body, haemolymph and ovary of P. japonica were detected. The results suggested that the vitellogenin was synthesized in fat body at the 2nd day after eclosion. The contents of vitellogenin in fat body, haemolymph and ovary increased quickly on the 4th day after eclosion and reached the peak stage approximately on the 8th day in adult stage.

采用间接竞争ELISA法，系统测定了龟纹瓢虫成虫期脂肪体、血淋巴和卵巢中卵黄蛋白的动态变化，结果表明：脂肪体是卵黄原蛋白合成的场所，卵黄原蛋白的合成始于羽化后第2天；脂肪体、血淋巴和卵巢中卵黄原蛋白的滴度在羽化后第4天开始迅速上升，至成虫期的第8天左右达到高峰期。

Methods: The expression level of the IL-10 and IFN-γ in the vaginal secretions from vulvovaginal candidiasis patients was detected by ELISA and compared with that from normal controls.

ELISA法检测初发组和复发组念珠菌性阴道炎的妇女阴道分泌物中 IL-10、IFN-γ的表达水平，以健康体检妇女为对照组。

Results: The result indicated ELISA method was more sensitive, but the reaction time was much longer, the process is more complex, and the special instrument operation was needed. The agglutinates testing method was simple, their results could be observed in the short time visually, but the sensitivity was nothigh.

结果：几种方法结果表明，ELISA方法更灵敏，但反应时间长，过程复杂，需特殊仪器操作；而凝集试验方法简单，可在短时间直接观察结果，但灵敏度不高。

It was experimentally found that the average titer of antiserums was more than 12800 by indirect ELISA.

以PA-OVA为包被抗原，采用间接ELISA检测抗血清，其效价达到

Methods Serum TNF-α and IL-6 level in 55 of lung cancer cases and 40 in normal control group were assayed by ELISA.

方法采用ELISA法测定55例肺癌患者和40例健康对照人血清中TNF-α和IL-6水平。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。