查询词典 ELISA

The fused dominant peptide antigen 38 kD-ESAT6-CFP10 might serve as a serodiagnostic candidate antigen, and the double antigen sandwich ELISA using the fused antigen might be used to detect the tuberculosis and improve the specificity and sensitivity of diagnostic reagent.

该种抗原优势肽段融合抗原有望成为结核病血清学诊断的重要候选抗原，并且双抗原夹心ELISA方法有助于提高检测的特异性和敏感性。

METHODS Serologic HBV infection markers of pregnant women and newborns were detected by ELISA and PCR.

孕妇和新生儿血清HBsAg和HBVDNA检测采用ELISA和PCR法。

The purified protein induced as antigen was used to immunize the rabbit, and the antiserum of WMV CP was prepared whose titer measured by ELISA was 1:6400; western blot analysis indicated that the antiserum could serologically react with CP.

以诱导的蛋白为抗原免疫家兔，制备了WMV CP抗血清，ELISA法测其效价为1/6400，Western blot分析能与CP发生血清学反应。

The immundogical function and cytokine levels in the serum of the tunor-bearing mice were determined by MTT colorimetry,hemolysis spectrophotography,improved Mayer's method and ELISA.

分别采用溶血分光光度法、改良的Mayer法及双抗体夹心ELISA法，检测荷瘤小鼠的免疫功能及细胞因子含量变化。

The short statured children were given combined pituitary hormone provocating tests, the corresponding serum GH,IGF-1 and urinary GH were determined by ELISA kits.

对垂体功能复合刺激试验生长激素分泌异常的56例矮小儿童，用ELISA方法检测相应的血清GH、IGF-1及尿中GH水平，并进行相关分析。

The expression changes of TNF-α and p65 mRNA expression were detected by reverse transcription-polymerase chain reaction, the TNF-α level in the supernatant was determined by ELISA, and the expression of p65 in AR42J cell lines was detected by Stept Avidin-Biotio Complex.

逆转录－多聚酶链反应半定量法观察肿瘤坏死因子/p65亚单位mRNA表达的变化；ELISA法检测TNF-α在细胞上清中质量浓度的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达。

In vitro transcripts from RNA5 wild type clone were co-inoculated into Tetragonia expansa with the RNAs of Hu3 isolate containing RNA1, 2 and 3. Following RT-PCR and Northern blotting results shows RNA5 could be biologically replicated in the leaves. The virus concentration of the isolate containing RNA5 in T. expanxa was higher than that of the isolate Hu3 evaluated by ELISA detection.

野生型RNA5的体外转录物与含Hu3(RNA1+2+3)分离物的总RNA混合接种番杏叶片的RT-PCR和Northern blot检测结果都表明，野生型RNA5能够在接种寄主体内复制，并且ELISA检测证明含有RNA5的番杏病叶中病毒浓度高于不含RNA5病叶的病毒浓度。

Methods: Leptin levels from serum and ascitic were measured in 67 patients with hepatic cirrhosis (among them complicated with SBP 32 and transudate group 35) by immunoradiometric assay, and the levels of TNF-α and IL-6 were measured by ELISA.

采用放射免疫法测定67例肝硬化腹水患者（SBP组32例，漏出液组35例）血清及腹水瘦素水平，同时采用双抗体夹心ELISA法测定血清及腹水的TNF-α、IL-6水平。

Through ELISA detection, vitellin"s synthesis and intake of female worm taking in artificial ovum Trichogramma"s pupas were both obviously reduced.

通过ELISA检测发现，取食人工卵赤眼蜂蛹的雌虫卵黄蛋白合成和摄取都明显减弱。

Methods To detect the antibody levels of tuberculoprotein 16KDa, 38KDa and LAM by Protein Chip and ELISA together, the sensitivity, specificity and total coincidence rate of the two methods were compared.

应用蛋白芯片识别仪分析结核病人血清中结核菌蛋白16Kda和38Kda以及脂阿拉伯甘露糖抗体含量，同时与ELISA法进行比较，判断其敏感性、特异性和总符合率。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。