查询词典 ELISA

Methods PPD from Mycobacterium tuberculosis taken as coating antigen,the antibody Of 116 patients with pulmonary tuberculosis ( thev were devided into positive bacteria group negative bacteria group and,remeasurement one afer treating)and 112 cases without pulmonary tuberculosis were measured by ELISA,to calculate the relev...

采用高纯度结核分支杆菌纯蛋白衍生物作包被抗原，应用ELISA检测临床诊断肺结核患者116例(其中分菌阳组、菌阴组、治疗后复检组)和112例非结核病患者，健康体检者的血清结核抗体水平；计算诊断试验的相关指标。

Methods This study contained a total of 16 culture groups, including one group as control and 5 groups treated by 5 drugs (calcium L-threonate, sodium L-threonate, alendronate, 17β-estradiol and calcium gluconate) each at the final concentrations of 10^(-9)mol/L, 10^(-7)mol/L, 10^(-5)mo1/L respectively. After 7 days, eight bone slices of every group were stained with toluidine blue and the areas of resorptive pits were analyzed under light microscope; the concentrations of C-telopeptide of type Ⅰ collagen CT(subscript x or Crosslaps in culture supernatants were measured by ELISA.

制备骨磨片，培养OC，并分别加入高中低浓度10^(-5、10^(-7)、10^(-9)mol/L的L-苏糖酸钙、L-苏糖酸钠、阿仑麟酸钠、17β-雌二醇、葡萄糖酸钙，实验设以上各浓度组及对照组（共16组，每组n=8），甲苯胺蓝染色一光镜观察分析骨片上骨吸收陷窝面积，ELISA测定上清液Ⅰ型胶原C-末端肽（CTx或Crosslaps）浓度。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

Accumulation experiments were performed with:(1) Scenedesmus quadricanda as single food;(2)a 50:50 mixture of Scenedesmus quadricanda and Microcystis aeruginosa based on carbon concentration; and (3) Microcystis aeruginosa as single food. MCs inside Bellamya aeruginosa were tested by ELISA, and a curve for the dynamic variation of MCs is obtained.

毒素积累试验分别以单一四尾栅藻、50%铜绿微囊藻+50%四尾栅藻的混合藻液和单一铜绿微囊藻3种处理投喂铜锈环棱螺达15天，用ELISA法检测，得到了微囊藻毒素在螺体内的动态变化曲线。

RT-PCR technique was applied to detect the expression of PAF receptor gene in ischemic semidark band cortex after MCAO and recanalization. At the same time ELISA method was used to determine the corresponding PAF values in plasm.

应用RT-PCR技术检测MCAO及再通后缺血半暗带皮质PAF受体基因表达，同时用ELISA检测对应血浆PAF值。

The bronchial asthmatic model was established by egg protein sensibilization and longterm inhalation provocation.The rats of each treatment groups were lavaged each day from the first time of provocation to execution.After 4 weeks of treatment,the rats were killed to obtain lung tissue for dyeing of HE.A computer assisted image analysis system was used to determine the thickness change of bronchus wall and smooth muscle.The contents of MMP9 and TIMP1 in lung tissue were determined by ELISA double antibody sandwich method.

除正常组外以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型，各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药，激发并给药4 w后处死大鼠，取肺组织HE染色，彩色图像分析仪测量支气管管壁厚度、平滑肌厚度，采用ELISA双抗体夹心法测定肺组织MMP9、TIMP1含量。

Results The chimeric protein reacted with anti HBs seral specially.The detection positive ratio of anti HBs is 77.5% and 68%respectively by ELISA and Wstern blot with chimeric protein as coating antigen,the control's was 62%.

结果：嵌和蛋白可与抗 HBs特异性结合，其ELISA检测抗 HBs阳性率为77.5%，Western blot的为68%，而对照试剂盒的为62%。

Results The chimeric protein reacted with antiHBs seral specially.The detection positive ratio of antiHBs is 77.5% and 68%respectively by ELISA and Wstern blot with chimeric protein as coating antigen,the control's was 62%.

结果：嵌和蛋白可与抗HBs特异性结合，其ELISA检测抗HBs阳性率为77.5%，Western blot的为68%，而对照试剂盒的为62%。

After 3 inoculations,the mice were sacrificed and their splenocytes and sera were collected.T cell subsets were analyzed by FCM.IFNγ in their cultural supernatants and seral IgG antibody against CEA were detected with ELISA.

FCM分析脾脏T细胞亚群；体外培养脾细胞，ELISA检测培养上清中干扰素γ的相对含量；同时测定小鼠血清CEA特异性抗体的滴度。

Dot ELISA using 10～30 kDa antigen might be a specific and sensitive serodiagnostic method for diagnoing pagumogonimiasis.

斯氏狸殖吸虫成虫 10～ 30kDa抗原的dot ELISA为斯氏狸殖吸虫病高度特异和敏感的血清学诊断方法。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。