查询词典 ELISA

To determine the infection frequency of CVX-NTU and CVX-Hu in pitaya, samples collected from Yangmingshan orchard were detected by ELISA and one tube multiplex RT-PCR. Up to 95% of tested samples were infected with CVX-NTU and 35% contained CVX-Hu.

另一方面为了解CVX-Hu与CVX-NTU两种不同分离株在红龙果分布的情形，利用ELISA及one tube multiplex RT-PCR对阳明山观光果园种植的红龙果进行检测调查，结果显示CVX-NTU感染较CVX-Hu普遍，前者高达95%，而后者占40%且都是复合感染。

The recombinant Hansenula polymorpha strains for secretory expression of HBsAg were screened and cultured in YPD, BMMY and MM media respectively. The expression levels of HBsAg were determined by ELISA, and the virus-like particles were observed by transmission electron microscopy.

将其电转化多型汉逊酵母尿嘧啶缺陷型宿主菌ATCC34438(Ura3-)，筛选分泌表达HBsAg的汉逊酵母工程菌株，并比较在YPD、BMMY、MM3种培养基中分泌表达HBsAg的水平，ELISA检测培养上清液中HBsAg的表达量，透射电镜观察类病毒颗粒。

The amount of dead mice,the amount of positive which detected by ELISA from stool,the content of sugar and natrium from stool,which the group of the leaf of Psidium guajava is under the control group of infected and the control group of treatment.

小鼠死亡数、粪便用ELISA试剂盒检测阳性数、小鼠的粪便Na+、糖含量，在番石榴叶组均明显低于感染对照组和治疗对照组。

In order to evaluate prevalence of Chlamydia psittaci in Beijing and other provinces around, 374 blood samples and 81 clinical suspected samples from Beijing, Hebei, Tianjin, Nei Monggol, Shandong, Shanxi and Henan were investigated by detecting for antibodies against C.psittaci using IHA and ELISA, and for pathogens using modified Ziehl-Neelsen staining and immunofluorescence tests.

对北京市周边6省份怀疑感染鹦鹉热嗜性衣原体的鸡鸭血清样品374份、病料81份，分别使用IHA诊断试剂盒、ELISA试剂盒以及抗酸染色试剂和荧光抗体诊断试剂进行了检查，以评价北京市及其周边地区家禽鹦鹉热嗜性衣原体的流行性。

Methods Pterygial samples were extracted and collected and the pterygial endothelial cells and pterygial fibroblasts were cultured alone, conditional and co-cultured to form different culture systems. The methods included that to select the suitable intensity of ultraviolet by MIT, to detect the changes of curves of growth about two kinds of cells by MIT and to explore the developments of protein and RNA of vascular endothelial growth factor and fibroblast growth factor-basic in three culture systems under ultraviolet whose intensity is 20 mJ/cm^2 by ELISA and RT-PCR.

收集翼状胬肉标本，采用血管内皮细胞和成纤维细胞单独培养、条件培养和共同培养的方法构建体系，用MTT法选择紫外线照射细胞的适宜强度；采用MTT法绘制强度20mJ/平方公分紫外线照射下细胞生长曲线；采用ELISA和RT-PCR检测紫外线照射下3种体系中细胞上清液和细胞中血管内皮细胞生长因子和成纤维细胞生长因子的蛋白和RNA含量变化。

Pullorum disease ; fowl typhoid ; blocking ELISA

鸡白痢；鸡伤寒；阻断ELISA ；单克隆抗体

Pyogenous meningitis; TM. tuberculous meningitis and 2.VME. Viral meningitis or encephlitis. The levels of TNFα were determined by RIA; The levels of G-CSF and SIL-2R were determined by ELISA.

TNFα用放免法，G-CSF和SIL-2R用ELISA法，检测25例PM、19例TM、22例VME脑脊液中 TNFα、G-CSF、IL-2R的含量，并与对照组对照。

Methods:establishing s180 tumor model,and establishing the model of pyretic syndrome by alcohol. the centrifuged blood was detected pge-2 by the method of radioimmunity,and observed the condition of hot syndrome.to detect il-8 in tumor tissue of s180 mouse be interfered in hot pathogenic factor by method of abc-elisa.

利用腋下种植s180肉瘤细胞株建立肿瘤模型，并用灌胃乙醇的方法建立肿瘤热证模型，利用放射免疫方法检测s180小鼠血清中pge-2的水平，采用双抗体夹心abc-elisa法测定热邪干预s180小鼠肿瘤组织中il-8的含量。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。