查询词典 ELISA

The ELISA titer of culturesupernatant were 2~9 and 2~(10), and ascites were 4×10~6 and 16×10~6, respectively.

细胞培养上清ELISA效价分别为2~9和2~(10)，腹水ELISA效价可达4×10~6和16×10~6。

Methods ELISA and DEA-ELISA were used to determine the titer and avidity of serum IgG antibody to Pg381 before and one month after periodontal treatment in 17 periodontitis patients and 6 healthy control subjects.

采用ELISA法，检测17例牙周炎患者治疗前及治疗后1个月及6名健康对照者的血清抗Pg381 IgG抗体滴度及亲和性。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

BALB/C mice were immunized with the purified PrV of MIN A isolate,mouse spleen cell were fused with myelomas NSo.Culture supernatants of hybridomas were screened by indirect ELISA and immobilization ELISA,positive colonies were cloned three times by limited dilution.

用纯化的伪狂犬病病毒免疫BALB/C小鼠，取脾细胞与NS_0骨髓瘤细胞融合，经间接ELISA和固相化ELISA筛选，3次有限稀释法克隆，获得了1株能稳定分泌抗伪狂犬病病毒单克隆抗体杂交瘤细胞株，命名为OH_6。

Titers and affinity constant of McAb were determined with indirect ELISA, and cross reactivity of McAb with indirect competitive ELISA.

用间接ELISA检测该McAb的效价和亲和性，用间接竞争ELISA检测与其他结构类似物的交叉反应率。

The ELISA assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of Aspergllius, while the other assay could only detect Aspergillus fumigatus of both clinical and environmental isolates.And no cross reaction with the cell culture of Penialllium marneffei and Candidas was observed with the two methods.

用mAbs-1建立的双抗体夹心ELISA法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mAbs-2)建立的双抗体夹心ELISA法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心ELISA法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

This ivp2-ELISA assay was compared with HA/HI and PPV ELISA kit of Cedi company ,result shows the agreement ratio is 97.2% and 91.2%.

采用iVP2I-ELISA对猪血清样品进行检测，结果显示iVP2I-ELISA与HI试验的符合率为97.2%，与国外同类试剂盒的符合率达到91.2%。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Capture ELISA method was used to determine isotypes of the mouse monoclonal antibody and the indirect ELISA method was performed to determine the titers of McAbs.

捕获ELISA法测定小鼠免疫球蛋白亚型，间接ELISA法测定腹水效价；用两步硫酸铵法和G蛋白亲和层析法来纯化腹水。

Methods: By capturing-ELISA and indirect-ELISA, we detected the level of HCMV-IgM and HCMV-IgG in 516 diabetes mellitus patients and 148 healthy. 516 diabetes mellitus patients were devided into 5 and 5 groups according to the courses of disease and age of disease onsets respectively.

运用捕获ELISA、间接ELISA对516例糖尿病患者及148例健康正常人HCMV-IgM、HCMV-IgG进行检测，并对比分析HCMV感染状态，根据糖尿病患者的初发年龄和病程的不同进行了分组探讨。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。