查询词典 ELISA

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

The cytolytic activity of CTL and NK were detected by variable MTT method. The content of IL-2 and IFN-γ in serum were measured by ELISA.

取MMC处理的colon26细胞、colon26/pMKITneo细胞和colon26/4-1BBL细胞，分别腹腔免疫BALB/c小鼠，采用改良MTT法测定CTL和NK细胞杀伤活性，取血清用ELISA法测定IL-2、IFN-γ下含量。

The cytolytic activity of CTL and NK were detected by variable MTT method. The content of IL_2 and IFN_γ in serum were measured by ELISA .

取MMC处理的colon26细胞、colon26/pMKITneo细胞和colon26/4_1BBL细胞，分别腹腔免疫BALB/c小鼠，采用改良MTT法测定CTL和NK细胞杀伤活性，取血清用ELISA法测定IL_2、IFN_γ含量。

Serum IgG titers against P.gingivalis antigens were measured by enzyme-linked immunosorbent assay. IgG avidity was measured by diethylamine dissociation ELISA.

血清IgG抗体滴度采用酶联免疫吸附试验测定；其亲和力通过二乙醇胺分离，ELISA测定。

The IFN-γ in the supernatant of dendritic cells was quantified elevatedly by ELISA.

以ELISA方法检测T-bet基因修饰的树突状细胞可以高表达IFN-γ。

Therefore, the object of this study is to investigate the prevalence of both enteric virus in Taiwan by molecular method (RT-PCR and ELISA).

因此，本研究的目的是以分子生物学的方法(RT-PCR和ELISA)调查轮状病毒与肠病毒在台湾的交叉流行期分布。

Methods: The concetration o f P-sel in plasma of 5 3 patients with liver cancer at different clinical stage, 22 patients with viral hepatitis B, 14 patients with liver cirrhosis and 30 normal volunteers were det ermined with ELISA method.

利用ELISA法检测了53例不同期肝癌患者、22例慢性乙型病毒性肝炎爱情患者、14例生活肝硬化爱情患者和30例西医健康人血浆中P-sel的含量。

Under inhibit tumor fall increment andalert confession The objective of this experiment is to prove the antitumor effect and its mechanism in vivo of Erysimum cheiranthoides. In this study we observe the ratio of inhibitory of mice bearing S180 and the effect of prolonging lives of mice bearing H22;the effect on immune function of mice bearing S180;the phagocytosis of MO cells; measuring serum TNF of mice bearing S180 with ELISA; the expression level of p53 in tumor tissue with ICH and the change of tumor cells shape with pathology test.

实验采用S_(180)、H_(22)两种瘤株，以昆明利，小鼠为研究对象，对体内的抑瘤作用、生命延长作用进行研究；对S_(180)荷瘤小鼠免疫功能影响的研究；采用碳粒廓清法研究该药对巨噬细胞吞噬功能影响的研究；采用ELISA法检测S_(180)荷瘤小鼠外周血TNF含量；采用免疫组化法测定S_(180)小鼠瘤组织中p53蛋白的含量并观察瘤细胞的病理形态。

Healthy dogs were inoculated with rabies vaccine and antibody titre was measured by ELISA and FAVN method from the 14th day. The immunized dogs were challenged with rabies virus BD06 strain.

将40只犬免疫兽用狂犬病疫苗后，第14 d静脉采血分离血清，分别采用ELISA法和国际贸易指定试验FAVN法检测血清样品，试验犬免疫第21 d攻击狂犬病毒BD06株。

In the examination of IgG_4 antibodies of 69 bancroftian microfilaremic cases by ELISA,the positive rate was as high as 97.10%at1:100 dilution,Showing a prominent elevation of IgG4 antibodies in filarial infection.

应用马来丝虫成虫抗原、鼠抗人 IgG_4单克隆抗体和碱性磷酸酶标记的羊抗鼠 IgG_4结合物做ELISA，对69份班氏丝虫微丝蚴血症者血清测定 IgG_4抗体。在血清稀释度1：100时阳性率高达97.10%。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。