查询词典 ELISA

ELISA law and result of analysis of law of gas phase color atlas have better consistency, proved the feasibility of the ELISA method that this laboratory establishs thereby.

ELISA法和气相色谱法分析结果具有较好的一致性，从而证实了本实验所建立的ELISA方法的可行性。

The monoclonal antibodies are specific to CSFV and can't bind to the normal samples such as pig's kidney and liver in indirect-ELISA and sandwich-ELISA.

在间接ELISA和夹心ELISA检测中单抗与猪瘟病毒呈特异性反应，与正常的猪、兔肾组织提取液及其血清Ig无交叉。

Objective To explore the clinical value of the titer of serum HP antibody in patients with gastric diseases.Methods Serous HP antibody was detected with ELISA and HP was cultured,tested with urase,and stained with Gram method on smears which was observed microscopically and compared with proteus protein-enveloped test.Results ELISA showed high sensitivity and specificity.Conclusion Serous ELISA detection of HP antibody can be used widely in clinical diagnosis and epidemical screening without endoscopy and biopsy.

目的 探索胃部疾病患者血清中的幽门螺旋菌HP抗体滴度的临床价值方法应用酶联免疫吸附试验ELISA法检测HP抗体与HP培养，尿素酶试验和涂片革兰氏染色作镜下形态检查相比较，并同时与变形杆菌蛋白包被测定法作比较结果显示该法具有敏感特异性强的特点结论该法无需常规内镜检查，活检查HP而单纯用血清学检查，可广泛应用于临床诊断和流行病学人群普查

According to the result, we believed that the reason resulted in the low sensitivity was that the marker residue compound MQCA structure has changed after conjugated with protein and the antibody induced by the conjugation could not recognize the free MQCA in the ci-ELISA. Therefore we designed some hapten which could reserve the marker residue structural speciality mostly. After synthesis route selection and optimum, the hapten 7-Hydroxymethyl-3-methyl-quinoxaline-2-carboxylic acid was synthesized, used 4-methyl-2-nitroaniline as the start material via six steps, such as oxidation cyclization, bromize reaction, hydrolysis, Beirut reaction, reduction, hydrolysis. This work established basis for producing the high affinity antibody and developing the sensitive ELISA residue detection method.

本论文认为导致ELISA检测灵敏度较低的原因，是残留标示物和蛋白偶联后的特征结构发生改变，诱导产生的抗体不能够对游离MQCA很好的识别，因此，本论文设计了能够最大化保留MQCA结构特征的系列半抗原结构，并对其合成路线进行了筛选和优化，最终以对甲基邻硝基苯胺为起始原料，经氧化环化、溴代、水解、Beirut缩合、还原、水解六步反应制备含羟甲基结构的MQCA衍生物7-羟基甲基-3-甲基-喹噁啉-2-羧酸，为制备对游离残留标示物高亲和力的抗体，高灵敏度的ELISA残留检测方法的建立奠定基础。

Result150of patients and infected sheep,Cattle,Swine serum positive rates of DAgS -ELISA were 91.4%,74.6%,and 66.7%re spective-ly. In the main, DAgS -ELISA gain adva ntage over of SAT and RBPT.

结果上述3种试验对健康人、羊、牛、猪共计140份血清检查均为阴性反应，用DAgS-ELISA对布氏菌感染人、羊、牛、猪共计150份血清检查，其阳性率分别为91.4%、74.6%、66.7%及66.7%，从总体而言，检查的特异性及敏感性，以DAgS-ELISA优于SAT及RBPT。

Genesee strives to establish its core product pipelines in antibodies and recombinant antigens to meet the needs of a wide variety of diagnostic techniques, such as latex agglutination assay, capture ELISA, double antigen ELISA, double antigen-mediated lateral flow immuno-chromatograph, chemoluminescence, multiplex microsphere assay.

公司致力于抗体和重组蛋白两种核心原材料产品的开发，以满足各种诊断技术的需求，如乳胶凝集试验法，间接法ELISA，双抗原ELISA，双抗原介导的胶体金快速诊断，化学发光法，蛋白液体晶片多重诊断技术。

An indirected ELISA was established with the purified recombinant protein, after the square matrix titrate determination best density is 2.43μg/mL, the blood serum best dilution ratio is 1:40,Compares with ten reagents boxes, proved we establish the ELISA method has the high specificity and the sensitivity.

以纯化的重组蛋白建立了初步的间接ELISA方法，经方阵滴定确定最佳包被浓度为2.43μg/mL，血清最佳的稀释比为1：40，与万泰试剂盒相比较，证明我们建立的ELISA方法有较高的特异性和敏感性。

The sandwich ELISA assay showsbetter sensibility and specificity than RT-PCR,hemagglutination inhibition assayand indirected ELISA.

与RT-PCR和间接ELISA法相比，双抗体夹心ELISA法具有敏感性高、特异性强的优点，适于临床检测使用。

Canine Leptospira antibody ELISA detection method was established through a study on canine Leptospira ELISA tests.

通过对犬用钩端螺旋体病ELISA检测方法的研究，建立了犬用ELISA钩体抗体检测方法。

The expression product was analyzed and its antigenicity was further studied by ELISA and Wstern blot method with chimeric protein as coating antigen.we used chimeric protein as coating antigen to test anti HBs with ELISA and Wstern blot in 58 seral samples.

通过ELISA和Western blot检测表达产物的抗原性，并以表达产物为包被抗原，通过ELISA和Western blot检测58份乙肝患者血清抗 HBs。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。