查询词典 ELISA

The application of uniform design for the optimization of ELISA method for determining parathion in soil was investigated and compared with the regular optimization method.

以对硫磷为对象，通过与常规方法相比较，对均匀设计法在ELISA分析方法条件优化实验中应用的适用性进行了研究。

In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10. Then the DNA fragments of map0862 and map2154c were spliced byoverlapping extension, yielding a fusion gene map0

为了建立一种有效的牛副结核病间接ELISA方法，本研究设计了两对引物，从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、，采用重叠延伸剪接技术(splice by overlapping extension，SOE)获得融合基因map0

Methods Saliva SIgA level was detected by ELISA in 45 children with recurrent parotitis and 36 children without recurrent parotitis.

方法采用ELISA法定量测定45例复发性腮腺炎患儿唾液SIgA含量，并与36例非复发性腮腺炎患儿作比较。

The results showed that the 20 phagocyte clones, which was selected at random after three times selection, could combine with BmVUB with ELISA analysis.

结果表明，3轮筛选后随机挑取20个噬菌体克隆，ELISA 鉴定表明20个克隆均可与BmVUB结合。

To detect concentration of EGF in mice phallus and serum with control group and test group using ELISA respectively.

ELISA法定量检测对照组和试验组小鼠阴茎皮肤组织和血清中 EGF的浓度。

The humanization and specificity of chimeric antibody was identified by ELISA and Western bloting, and the tumoricidal activity of the expressed chimeric antibody was detected by tetrazolium salt phenazine methosulfate assay.

采用ELISA和Western bloting法鉴定抗体的人源性和抗体-抗原结合活性，四唑盐/吩嗪硫酸甲酯比色法检测抗体的生物学活性。

In addition, a discuss about the use of indirect ELISA in the phytoplankton research was also made in the paper.

此外，还对间接ELISA在浮游微藻检测中的应用作了初步探讨。

Methods The rabbit model with hyperhomocysteinemia was established by L-methionine subcutaneouse injection. The changes of white microthrombus and microvascular diameter in pia mater microvessel were observed by using cranial window method and closed microcirculation detection system, The concentration of Hcy was detected by high performance liquid, The level of von Willebrand factor, endothelin-1 (ET-1) were assayed by ELISA. The content of NO was measured by nitrate reduction method. Meanwhile, the relationship between the changes of microvascular diameter and blood concentration ET-1, NO was analyzed.

采用L-蛋氨酸皮下注射法建立HHcy家兔模型，应用"颅骨窗口法"和微循环闭路检测系统观察其软脑膜微血管中白微栓数量及微血管口径变化；采用柱前衍生反相高效液相色谱－荧光法检测同型半胱氨酸浓度；平行观测血管内皮功能指标血管性血友病因子抗原（vWF，用酶联免疫吸附法）、血浆内皮素－1ET-1，用ELISA）、血清一氧化氮水平；并对HHcy所致ET-1、NO血液浓度变化和微血管口径变化进行相关分析。

The average positive rate of Porcine Contagious pleuropneumonia is above 63% using the Apx II -ELISA method to detect 2503 clinic serum samples.

利用建立的ApxⅡ-ELISA诊断方法对2503份临床送检的各地猪血清进行检测，测得猪传染性胸膜肺炎的阳性率平均为63％以上。

Methods: the hbv transgenic mice were generated by microinjection of 1.3 copies hbv genome into the pronucleus of fvb/n zygotes. the pcr assay,elisa,rt-pcr and immunohistochemical methods were used to detect the hbv integration,replication and expression in the transgenic mice.

采用受精卵显微注射法，制备hbv转基因小鼠，用pcr，elisa，荧光定量pcr和免疫组化的方法研究hbv基因在转基因小鼠体内的整合、复制和表达情况。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。