查询词典 ELISA

Six healthy no fascioliasis goats confirmed by fecal examination and Dot-ELISA were chosen and randomly divided into Ⅰ,Ⅱ,Ⅲ groups. Each goat of Ⅰ,Ⅱ groups was orally dosed once 200 and 500 Fasciola hepatica metacercariae and during 11 weeks after infection used for the experiment study. Blood sample was collected by jugular vein weekly, to determine IL-2 and TNF-α levels in serum and IL-2 in supernatant with proliferation of PBL of goats infected with Fasciola hepatica.

试验选择6只健康白山羊（经粪便学检查和Dot-ELISA检测，确认无肝片吸虫感染）随机分成Ⅰ、Ⅱ、Ⅲ组，其中Ⅰ、Ⅱ组山羊每只分别1次口服接种200、500个肝片吸虫囊蚴，Ⅲ组作为不感染对照组，每周颈静脉采血1次，连续11周，检测肝片吸虫感染后山羊血清中IL-2、TNF-α水平的变化和外周血淋巴细胞分泌IL-2的能力。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

Objective To explore the possible application of ELISA for detection of Fumonisin contained in fodder and food.

目的 探讨以ELISA法检测粮食、饲料中伏马毒素的可行性。

Immunize BALB/c mice with the produced GAMP-ADH-Pep10 conjugate after purification, determine the serum IgG level against GAMP by indirect ELISA, and analyze the immune suppression induced by carrier protein.

纯化后免疫BALB/c小鼠，用间接ELISA法检测小鼠血清中抗GAMP IgG抗体水平，并分析载体诱导的免疫抑制率。

The geometric mean titers of ELISA antibody and NA were 3.8 EU/ml and 5.7 IU/ml, respectively.

ELISA检测的抗体和中和试验检测的抗体平均滴度分别为3.8EU/ml和5.7IU/ml。

With rabbit antiserum against ECP of reference strain JEL4,ECPs of 25 Et strains were detected by Dot-ELISA; additionally,alI strains had been detected both on their virulent factor Excellular Products, including the hemolysin and extracellular protease, and on their pathogenicity to mice and Xiphophorus helleri.

以Et强致病株JEL4的胞外产物ECP为抗原制备兔抗血清，用于Dot-ELISA检测25株Et的ECP，同时对Et的溶血素和胞外蛋白酶进行检测，结合小鼠和剑尾鱼的致病性试验。

Antibody anti-pertussis toxin and anti-filamentous hemagglutinin antibody were measured by using ELISA method.

抗百日咳毒素抗体和抗丝状血凝素抗体采用酶联免疫吸附试验ELISA方法。

In the paper, a preliminary study was made to analyze the interaction between hemocyanin, which was purified from shrimp P. vannamei obtained from Shantou, and anti -human antibody. The methods of Sephadex G-75 column chromatography, PAGE, Dot-ELISA, SDS-PAGE and Western-blotting were used.

以采自汕头的南美白对虾血清为研究对象，运用Sephdex-75柱层析、PAGE、Dot-ELISA、SDS-PAGE和Western-blotting等方法，探索血蓝蛋白与抗人IgG之间的相互作用。

Methods ELISA was used to detect EPO level in 40 patients with CRF and anemia before and after treatment and 30 healthy controls (normal renal function without anemia), and serum Hb, RBC and HCT levels were determined by hemocyte analyzer.

用ELISA法检测40例CRF伴肾性贫血患者治疗前后和30例健康对照者血清中EPO、血细胞分析仪检测Hb、RBC和HCT水平。

This paper describes a new immunization program of hog cholera, which was determined by OD value of PPA-ELISA test.

本文根据PPA-ELISA测定的猪瘟弱毒抗体OD值来决定猪瘟免疫程序。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。