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Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

Carefully constructed from two photographs( ISS020-E-038481, ISS02-0E-038482) taken duringspace shuttle orbiter Discovery's latest visit to the orbiting outpost, the 3D anaglyph creates thecompelling illusion that you can actually reach out and take hisgloved hand.

这张影像来自两张照片（ ISS020-E-038481， ISS02-0E-038482），都是在发现号最近国际空间站之旅拍摄，3D 立体影像让人有一种强烈的感觉，伸出手来就能抓住他的手。

Piano Trio No. 2 in E minor, Op. 2: I. Andante - Moderato 07:15

E小调第二钢琴三重奏曲，作品67

Exergy means available energy, any form of energy can be expressed as the sum of exergy and anergy E=Ex+An.

有效能或称是指能量中的可利用部分，任何一种形式的能量都可以表示为：E=Ex+An。

There was a positive relationship between SPF that is higher than 15% and tumor size, differentiation and LN metastasis. And there was a significantly difference between carcinoma and noncarcinoma when the expression of E-cadherin, DNA aneuploid rate and SPF were analyzed.

随着肿瘤的演进和异质化，TIMP-2和E-cadherin 的异常表达及DNA异倍体和高S期分数也相应逐渐增加，提示它们在胃癌演进过程中起着关键作用，可以作为胃癌生物学行为的客观标志物。

I have constructed and screened a cDNA library of Xenopus oocytes with E-cadherin probe. One of the four cadherin-related mRNAs analyzed is possibly localized to the animal pole.

在本篇论文中，我们建立了蛙卵母细胞的cDNA library，并利用E-cadherin 探针从中选殖到几个cadherin-related基因，在已分析的四个基因中有一个基因在卵母细胞中有不均匀分布发生。

Here is an e-mail I just received. It was, of course, anonymous

这是我刚刚收到的e-mail，当然是匿名的

E Researchers tested small areas of the anteater's snout in order to ensure that only electroreceptors were responding to the stimulus.

研究者检验了小范围的A的S为了确定只有E接收器才回应刺激物

During the summer monsoon the center of the anticyclonic eddy, breaking off from the Kuroshio bend, can reach about°E and the eddy shedding periods are mainly 40-120 days.

夏季风期间，从黑潮弯曲中脱落的反气旋涡的中心位置可以达到117°E附近，反气旋涡脱落的周期集中在40-120天间。

Because if you achieve the E antigen seroconversion, you can stop the drugs in 70-80% of the patients.

因为如果你获取了E抗原的血清转化后，你就能在70-80%的患者中停止药物治疗。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新