查询词典 DNase

METHODS: Porcine cornea stromata were processed by serial digestion of trypsin, DNase and RNase, and then were implanted into corneal stroma layer of New Zealand rabbits.

将用去垢剂联合酶的方法脱细胞处理的猪角膜基质材料植入兔角膜基质囊袋中，进行裂隙灯观察并照相。

However, the molecular mechanism is still unknown. Here the binding patterns of GATA factors (GATA-1 and GATA-2) to the regulatory elements of human β-globin gene were examined with the nuclear extracts from the HU-induced and uninduced HEL cells.

结果表明，羟基脲诱导后，与人β—珠蛋白基因5'远侧端DNaseⅠ超敏感点2核心序列以及近侧端启动子序列相结合的GATA-1蛋白因子量明显增多，而GATA-2蛋白因子量明显减少。

In uninduced HEL cells, GATA-2 transcription factor can specifically bind to the regulatory elements of human β-globin gene, including the proximal regulatory element (theβ-promoter) and the distal regulatory elements (the DNaseⅠ hypersensitive sites in the locus control region, HS2-HS4 core sequences).

在非诱导的HEL细胞的核蛋白中，能够与β—珠蛋白基因的近端启动子调控序列及远侧端LCR调控序列（HS2，HS3和HS4核心DNA序列）相结合的蛋白质主要是GATA—2转录因子，而在羟基脲诱导的HEL细胞核蛋白中，与上述调控序列相结合的蛋白质主要是GATA—1转录因子。

This probe hybridizes to a PstⅠ restriction fragment of 3. 2kb in intact chromatin.

其次用DNaseⅠ处理Jurkat细胞核后，纯化基因组DNA，经PstⅠ限制性内切酶酶解并作Sothern blot分析。

The plasmid DNA remained intact and could be stored at 4℃ for at least one month in liposomes, it was also resistent to DNase I digestion.

重组质粒DNA在脂质体的制备过程中保持了稳定性，并能抵抗DNA酶的水解破坏作用。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

Deoxyribonuclease II is an important acid endonuclease in lysosomes, nuclei and various secretions in many animals.

DNase II是核酸内切酶，在细胞内主要位于溶酶体、细胞核及各种分泌排泄物中，酸性条件(pH4.5–5.5)发挥最大活性。

Results The results showed that the pDNA was completely degraded within 1 minute in 5U/μl of deoxyribonuclease solution at 37℃. While the chitosan with MW more than 200000 showed favourable protective effect on DNA.

结果 在人体温度(37℃)下，浓度5U/μl的DNase Ⅰ在1min内即可降解没有保护的质粒DNA，而相对分子质量为200000以上的壳聚糖对质粒具有良好的保护作用。

MMC possesses several types of enzyme activity, such as N-glycosidase, RNase and deoxyribonuclease activities. All its biological activities may be related to the ribosome-inhibiting activity of the protein.

MMC属于Ⅰ型核糖体失活蛋白，具有N-糖苷酶、RNase及DNase活性，α-MMC的多种生物功能可能都与其核糖体失活的性质有关。

Two substitution derivates of β CD His and β CD His 2 was synthesized and characterized respectively,which simulate deoxyribonuclease,their activity of hydrolysis deoxyribonucleic acid are compared with DNaseⅠ′s.

合成了β-环糊精的组氨酸一取代和二取代衍生物(简写β- CD- His和β- CD- His2 )并对其结构进行了表征。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。