查询词典 DNA

X According to study of two extraction methods, qualification and quantity of DNA from CTAB method are better than SDS method among two-needle pines.?

通过对两种DNA提取方法的比较研究，认为利用CTAB法提取两针松的DNA在质和量上都高于SDS法。

Results The tail formation rate of model group was significantly higher than the rate of control group (P＜0.05) with a large number of Ⅲ and Ⅳ DNA damage cells. The tail formation rate of dichloromethane extraction groups were both significantly lower than the rate of model group (P＜0.05). p53 mRNA expression of model group was higher than the rest except the crude Cornus Officinalis group.

结果 D-半乳糖致衰模型组小鼠骨髓细胞DNA拖尾率高于正常对照组(P＜0.05)，且Ⅲ、Ⅳ级损伤细胞数较多；山茱萸生品及制品二氯甲烷萃取部位组小鼠骨髓细胞DNA的拖尾率均低于D-半乳糖致衰模型组(P＜0.05)。D-半乳糖致衰模型组小鼠骨髓细胞p53基因的表达高于正常对照组、阳性药组及制品组。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

The tester DNA sample was prepared from MGC 803 and the driverDNA from BGC 823.After two rounds of RDA,seven specific fragments existing inMGC 803 were isolated.

以MGC803作为检测DNA，BGC823作为驱逐DNA，通过两轮的筛选获得了7条MGC803细胞系特异扩增的基因片段。

Behind the enzyme, the DNA double helix re-forms, stripping off the newly synthesized RNA strand.

在多聚酶后， DNA 双螺旋又重新形成，新合成的 RNA 链也从 DNA 分子上脱落。

The dose-dependent experiment revealed that Id1 antagonised E47 and Ets1 mediated transcriptional activation in a dose-dependent manner. With pGL3-870 as a template, site-directed mutagenesis of E-box and / or ETS-binding site was introduced into p16 promoter. E47 could not activate the transcription of p16 promoter with E-boxes mutated, and Ets1 could not activate the transcription of p16 promoter with EBS mutated. The synergy effect between E47 and Ets1 would occur only if the E-boxes and EBS were present. These facts demonstrated that E47 and Ets1 proteins were not recruited to the promoter by binding to other cis-elements or through interaction with other protein factors, however, they bound to DNA directly through respective consensus sequences in p16 promoter.

以pGL3-870为模板，构建了E-box或/和EBS点突变的p16启动子报告基因载体，将野生型和突变型的报告载体分别与pI-E47、pI-Etsl以及pI-E47+pI-Ets1共转染，发现E47和Ets1所诱导的转录激活作用依赖于启动子DNA上存在的各自的特异结合序列，二者的协同作用也只有当两个结合位点都存在时才会发生，说明E47和Ets1不是与其它作用元件或其它蛋白质因子相作用而被招募到该启动子的，它们是通过启动子上各自的特异结合位点与DNA直接结合的。

objective to explore the effect of different anticoagulants on quantitative assays for hbv dna using real-time pcr technique.

目的 探讨用实时荧光定量pcr技术检测乙型肝炎病毒dna拷贝数时，不同抗凝剂对乙型肝炎病毒dna定量检测的影响。

And H.Cannabinus were introduced into the upland cotton Si 2 by Ar +implantation and DNA solution trickling method.

通过30keV不同剂量的Ar＋注入和DNA溶液浸滴法将比克氏棉和红麻DNA导入泗棉2号。

After boiling and centrifuging, the supernatants reserved DNA. The blood blot of specimen is easy to be obtained and reserved in low cost. The experiment technique is high in efficiency .

甲醇法与其它3种制备模板DNA的方法比较，具有提取的模板DNA质量较高，血标本容易获得、保存且成本低，制备效率高，而且实验方法简单易行等诸多优点。

Considering this model in terms of a cross-section through the nucleosome, in Figure 19.5 we see that the two circumferences made by the DNA lie close to one another.

考虑通过核小体区域部分的模型，在图27.5我们看到DNA围成的这两个圆周靠得很近，圆柱体高度6nm，两圈DNA占了4nm（每条直径2nm）。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。