查询词典 DNA

The results showed PERV proviral DNA and mRNA could be detected in all 31 individuals by PCR and RT-PCR, respectively. env-A, env-B and env-C were only detected in 2 individuals, while in the other 29 individuals, only env-A and env-B but not env-C was detected. The quantity of DNA in PCR amplification of PERV genes should be more than 15 ng.

PCR和RT-PCR结果表明，所检测的31头沙子岭猪均带有PERV前病毒DNA，耳样组织中均有PERV mRNA表达，其中有2头个体携带 env-A、env-B、env-C 3种囊膜蛋白基因，而其余的29头个体只带有env-A、env-B 两种囊膜蛋白基因，未检测到env-C基因。

Circulating nucleic acids are extracellular nucleic acids found in cell-free serum, plasma and other body fluids from healthy subjects as well as in patients. The ability to detect and quantitate specific DNA and RNA sequences has opened up the possibility of diagnosis and monitoring of diseases, especially in the field of cancer.

循环核酸(circulating nucleic acid， CNA)是指存在于血液等体液中的细胞外游离DNA和RNA，与生理和病理状态下的细胞代谢密切相关，循环DNA和RNA水平的检测及其在基因诊断等方面的应用对于恶性肿瘤等疾病的诊断和监测具有十分重要的意义。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

Study on systematic taxonomy of isolated halophiles had been done using polyphasic technology including morphological and cultural characteristic obserbations, physiological and chemical tests, analyses of chemtaxonomy and G+C content, DNA-DNA homology and 16S rRNA sequences.

并对分离到的嗜盐菌菌株采用形态特征、培养特征观察，生理生化测定，细胞化学组分分析，DNAmol％和DNA同源性测定，以及16S rDNA全序列分析等多项分类技术对其进行系统的分类研究。

Current advance of Taq DNA polymerase improvement and application in medicine has been discussed in this paper.

本文介绍了Taq DNA聚合酶的结构与功能改造的研究现状，并展望了Taq DNA聚合酶在医药领域的应用前景。

METHODS: Proliferation, DNA proliferative fraction and DNA ploidy were analyzed by flow cytometry using standard techniques.

应用流式细胞术，分别分析胃癌细胞群体的增殖、DNA增殖分数和倍性。

objective to explore plasmid dna extraction and purification methods as well as measure plasmid dna.

目的 探讨提纯质粒dna的方法并进行检测。

The machine lowers the reaction temperature to allow the DNA primers to hydridize or 'anneal' to the DNA template.

在完成一轮合成后，从最初的一个模板分子得到了两个 DNA 分子。

The machine heats the reaction once again, denaturing the DNA molecules – including the newly made ones – then cools to allow annealing of primers and synthesis.

PCR仪又一次加热使DNA分子变性──包括新合成的分子──然后降温使引物退火并进行DNA合成。

The UBC-868 is the most efficient primer which generates 16 polymorphic bands, including the most significant 900 bp polymorphic DNA fragment. The 900 bp fragment is specifically observed in vegetable soybean varieties which have black seed coat, except the yellow seed coat variety Kuo China.

其中以UBC868扩增出16个DNA条带为最多，且於电泳胶片上900 bp处有一明显的多型性条带，此DNA条带除了来自中国的Kuo China品系外，可视为常出现在黑色种皮毛豆中。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。