查询词典 DNA

The standard curve was constructed by using gene chip after the methylated and unmethylated DNA were mixed at different ratio. Then treated samples of U266 cells were dotted on gene chip, obtained results were compared with standard curve to get the quantitative results.

采用基因芯片的方法，将完全未甲基化的DNA和完全甲基化的DNA混合后制成标准曲线，将U266细胞株样本处理后点在芯片上与标准曲线进行比较后得出定量检测的结果。

Dna was extracted from 12 frozen tissues and 35 paraffin embedded tissues, then treated by sodium bisulfite and pcr amplified by specific primers for methylated versus unmethylated dna.

将提取的dna经亚硫酸盐处理后，用甲基化特异性pcr（methylation specific pcr，msp）方法检测blu和arf基因启动子的甲基化状态。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

The 9 specimens were not confimed to livae viable organisms of Chlamydia trachomatis. The debris of nonviable Chlamydia trachomatis DNA was exluded from urinogenital tract at about one month.

结论沙眼衣原体感染患者在服用抗生素后，PCR阳性不能肯定为有活性的沙眼衣原体存在，因其死亡的DNA片段可在体内存留1个月，只要有DNA片段存在，PCR检测均为阳性。

DNA extraction quality of Venturia inaequalis using CTAB method,SDS method,Parker's method and modified SDS method was detected by ultraviolet spectrometer.

采用CTAB法、SDS法、Parker法及改进的SDS法提取苹果黑星病菌的DNA，经紫外分光光度计测定，改进SDS法提取的DNA得率较其他3种方法高，约为Parker法的2倍。

In pH2.5 potassium hydrogenphthalate buffer solution, a supermolecular can be developed betvveen Cu-TZAMB complex and DNA, which can decrease the cathodic peak of this complex. This supermolecular complex was testified to be intercalating bound to the DNA by cyclic voltametric method, salt effect and the UV-VIS spectra.

通过循环伏安法、盐效应以及紫外-可见吸收谱证实了是由Cu-TZAMB络合物与DNA形成一种插入式的电惰性结合物而使其峰电流下降。

Tail Length, Tail Moment, Tail DNA Percentage, and correction rate were compared between the improved software and the original one, and epidemiological indices for the improved software were also calculated and analyzed, including sensitivity, specificity, Youden index, crude agreement, adjusted agreement, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio.

比较改进前后的尾长、尾矩、尾DNA百分含量上的差异、改进前后分析正确率差异、以及改进后的软件以不同DNA损伤级别为截断值判定阳性结果的灵敏度、特异度、Youden指数、粗一致性、调整一致性、阳性预测值、阴性预测值、阳性似然比和阴性似然比。

Phylogenetic analysis of chloroplast matK gene from Zingiberaceae for plant DNA barcoding［J］.

适合中药材DNA条形码分析的DNA提取方法的研究［J］。

Experiment was conducted with mature leaves of Zizyphus jujuba Mill. cv. Chuangan (stored at -20℃,-70℃, dried with silica gel) and 3 methods, including CTAB, high salt precipitation and high salt and low pH were used for total DNA extraction for studying their effects on DNA extraction.

以串杆枣的成熟鲜叶为试材，分别采用-20℃、-70℃和硅胶脱水干燥3种方法保存样品，采用简易CTAB法、高盐沉淀法与高盐低pH值法分别对3种方法保存的样品及对照样进行了基因组DNA提取效果的比较分析。

Methods Polymerase chain reaction was used to detect HPV 16 DNA and point mutation at condon 12 of Ha-ras oncogene.

应用多聚酶链反应技术扩增HPV16 DNA和Ha-ras癌基因相关片段，分别采用2％琼脂糖凝胶电泳和限制性片段长度多态性分析，检测口腔癌中的HPV16 DNA和Ha-ras癌基因第12位密码子的点突变。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。