查询词典 DNA

By using an empirical potential of free energy on dsDNA which was obtained experimentally by Strey, a relation between total energy of chip and some factors, such as nanogeometric, physical. chemical characteristics of DNA, geometric dimension, macroscopical mechanical properties of chip, etc was formulated under the consideration of mechanical energy of microcantilever beam, electrostatic energy, configuration entropy, and osmotic energy of gene-layer.

首先，在考虑微悬臂梁机械能和基因层静电能、构型熵、渗透能的情况下，借助Strey通过实验得到的双链DNA自由能的经验势，建立了DNA纳观几何、物理、化学特征以及芯片几何尺寸、宏观力学性能等因素与基因芯片总能量之间的关系。

We had preliminary researched on the mechanism of Stylosanthes guianensis cv. Mineirao resistant to anthracnose, a specific fragment of SG2950-1 was acquired from genome DNA of Stylosanthes guianensis cv. Mineirao by the technique of AFLP in our laboratory, we acquired both ends of the fragment of SG2950-1 by RACE from Stylosanthes guianensis cv.

我们已经对Mineirao柱花草的抗病机理进行了初步研究，并用AFLP分子标记技术从其基因组DNA中获得了一特异片断SG2950-1，利用快速扩增cDNA末端技术将基因组DNA特异片段的两端从Mineirao柱花草扩增出来，经测序后拼接得到MSRA基因的全长序列。

The results indicated that the DNA damage could be accelerated by Fenton reaction in the presence of ascorbic acid but was inhibited slightly by hydroxyl free radical scavenger such as mannitol or dimethyl sulphoxide under the experimental conditions.

试验结果显示，抗坏血酸存在时可以加速Fenton反应对DNA的损伤，而在同样条件下，自由基捕获剂甘露醇或二甲基亚砜对由Fenton反应造成的DNA损伤的抑制作用却不是很大。

The result is consistent with previous studies. Whereas, tapetal cells of sterile anthers were disintegrated since tetrad cells were disjoined, indicating that disintegration of tapetal cells is earlier occurred in sterile anther than that in fertile anther. DNA ladder revealed that the cleavage of nuclear DNA into oligonucleosome-sized fragments to

结果表明，在花粉母细胞减数分裂后的四分体解离时期，不育花药的绒粘层细胞较可育花药提前开始裂解；在不育系S-Mo17rf3rf3花药和花粉S-(rf3)中均明显出现PCD过程的DNA片段化以及线粒体细胞色素C外渗的现象，证明了玉米CMS-S的花粉败育与花药绒粘层细胞的提前凋亡和小孢子细胞的细胞程序性死亡有关。

In PCR detection systems, the DNA of recombinant plamid of TCK was used for safely positive reference and genomic DNA of the healthy wheat and rye was used for negative reference.

选择重组质粒作为检测中的阳性对照，健康小麦和健康黑麦基因组DNA作为阴性对照。

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast.

摘 要 应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化，发现药物在一定浓度作用下，DNA指数无明显变化；丹参使C2-M期细胞分布增多，G2-M期延长；川芎嗪使G2-M期细胞分布增多，G2-M期、S期延长；药物使细胞群体倍增时间延长，与浓度呈明显直线正相关。

Transcriptional factors play an equivalent role in plant during drought adaptation and cold acclimation. In order to study the expression pattern of CB F4 gene promoter and explore its potential application in plant genetic engineer ing, CBF4 promoter fragment was amplified by PCR method from Arabidopsis thalian a genome DNA.

根据已有文献及公开的拟南芥基因组序列，利用PCR方法从拟南芥基因组DNA中扩增得到了转录因子CBF4上游的DNA片断，对其进行序列分析表明与GenBank序列高度同源，同时对其进行初步的功能分析。

Methods: Eleven B-CLL patients were studied. Leukemic lymphocytes with (n=8) or without (n=3) P2z receptors were exposed in vitro to ATP, benzoylbenzoic-ATP, 2-methylthio-ATP(2MeSATP), adenosine-5′-［γ-thio］ triphosphate, and other nucleosides for 8h. Apoptosis was detected by electron microscopy, agarose gel electrophoresis, and quantitative TdT assay. Results:Apoptosis was detected only in leukemic lymphocytes with P2z receptors. By using the quantitative assay, ATP-inducing DNA strand breaks were found to occur specifically for BzATP, ATP and 2MeSATP, but not for ATP-γS and other nucleosides.

将表达P2z受体［P2z］与不含P2z受体［P2z］的两组CLL细胞分别同1.0mmol/L三磷酸腺苷体外培养8小时，以电镜、DNA凝胶电泳和定量DNA 3′端TdT法检测细胞凋亡；并对ATP、苯甲酰苯甲酸ATP、2-甲基硫ATP(2MeSATP)、γ-硫代ATP及其它核苷的诱导效应和氧化型ATP、1-［N，O-二(5-异喹啉碘酰基)N-甲基-L-酪氨酰］-4-苯哌嗪KN-62）的抑制效应做定量研究。

Results: Under the compression with a magnitude of 15KP、 30KP, the proliferation of HPDLFs greatly increased, and the percentage of the content of DNA of cells in S phase increased tooP

结果：①。OKP、15KP、30KP、45KP的持续性静压力，加力1小时后，体外培养的人牙周膜成纤维细胞增殖活性的变化为：0～15～30KP，增殖指数以及S期细胞DNA百分比都显著增高(P＜0.05)；45KP压力下，增殖指数及S期细胞DNA百分比明显降低。②。

Torenia fournieri genomic DNA was digested with DraI、EcoRV、PvuII、SmaI respectively. A special adaptor was ligated to the ends of the digested DNA fragments as a template for adaptor PCR. With the adaptor and TfPLC1 gene-specific primers, bands of 798bp and 813bp upstream of TfPLC1 were obtained successively.

应用衔接头PCR技术，以蓝猪耳全基因组DNA分别经DraI、EcoRV、PvuII、SmaI消化后与衔接头连接产物为模板，用衔接头引物和TfPLC1基因的特异引物经过多轮的巢式PCR，先后克隆到两个大小为798bp、813bp的TfPLC1基因上游序列；经测序、blastn比较分析和拼接得到一个蓝猪耳TfPLC1基因的启动子序列，共1432bp。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。