查询词典 DNA

ABSTRACT: Random amplified polymorphic DNA was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.

本文对采自湖南莽山的26种鹅膏菌属真菌进行了随机扩增多态性DNA分析，40个随机引物中筛选出扩增效果较好的6个引物，每个引物能产生1～10条DNA条带，获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来，通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20―60%之间，平均链锁聚类分析可将26种鹅膏菌分为二大类，且一些具菌环和苞状菌托的种类聚在一起，与形态分类基本相吻合。

The term is particularly used to describe the double helix of the Watson-Crick DNA model.

尤其用于描述沃森-克里克 DNA 模型的双螺旋。参见 DNA

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

In any animal, every cell contains exactly the same DNA, and that DNA comes from the zygote.

任何一种动物，每个细胞都包含着来源于受精卵的完全相同的DNA。

The application of Alit sequence in forensic genomics, include DNA quantitation, race determination, species and gender identification, personal identification, paternity testing and whole-genome amplification.

目前，Alu元件在法医DNA分析中的应用包括：DNA定量、种属鉴定、种族鉴定、性别鉴定、个体识别和亲子鉴定，以及全基因组扩增等。

The synthesis of molecular beacon. CdTe QDs, DNA and EDC were added into the phosphate-buffered saline solution, reacted for 24 h at room temperature. In this period, carboxyl on the CdTe reacted with amido on DNA.

二、在PBS缓冲溶液中，将CdTe量子点、DNA和脱水剂EDC混合，于室温下反应24小时，CdTe量子点表面的羧基与DNA5'端的氨基缩合，合成分子灯塔探针。

All cases were verified by the DNA of chorion,amnionic fluid and umbilical cord blood.The final accuracy was 100%.Conclusion: PCR is a reliable and accurate technique for fetal gender determination in pregnancy through analysis of fetal DNA in maternal plasma.

通过本实验的研究，利用苯酚氯仿法建立了一种有效、简便、经济的从血浆中提取胎儿DNA的方法，并通过调整PCR反应体系，成功从母体血浆中扩增出胎儿特异的SRY基因，最后经绒毛、羊水、脐带血以及出生胎儿验证实际性别。

A personal interview was done and material for anogenital HPV DNA detection was obtained with swabs. HPV DNA was detected by polymerase chain reaction.

结果：600例研究对象的HPV DNA总阳性率为14.50%（87/600），其中性病患者HPV阳性率［23.90%（38／159）］显著高于非STD就诊者［11.11%（49／441）］（P0.05）。

Pupal DNA of Antheraea yamamai, which had been irradiated by using He-Ne laser, was analyzed by RAPD. The results were that special bands increased, genetic distance became wider and DNA polymorphisms were related with GC content.

对He-Ne激光辐照天蚕卵的当代蛹进行RAPD分析结果显示：辐射区内独特带型增多，遗传差距增大，其DNA多态性与GC含量有关。

An optimized PCR reaction system for ISSR analysis was established:PCR was performed in a 20μl reaction mixture with 5μmol/L DNA, 2.5mmol/L Mg~(2+), 0.3mmol/Lof each dNTP, 300nmol/L primer, 1U Taq polymerase and 1×Buffer. Using 9 ISSRprimers,the genetic diversity among 23 Arrhenatherum elatius L.

3遗传多样性研究结果表明：通过模板DNA、引物、Mg~+、dNTPs、Taq酶五种ISSR反应体系的影响因素进行L_(16)(4~5)正交试验，建立了高燕麦草ISSR反应体系：20μl的反应体积中含有模板DNA 5μmol／L，引物300nmol／L，Mg~+2.5mmol／L，dNTP 0.3mmol／L，Taq酶1U及1×Buffer。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。