查询词典 DNA

The AC2 gene fragment of cotton leaf curl virus was obtained from total DNA of CLCuV infected tobacco leaves by polymerase chain reaction, and the amplified DNA fragment was cloned into vector.

以棉花曲叶病毒侵染的烟草叶片组织总DNA为模板，通过聚合酶链反应扩增CLCuV的AC2基因片段并插入克隆载体。

It showed that thymidine could have special effect on cells in that replication in cells suffering thymidine treatment slow down substantially but do not stop DNA synthesis. This phenominon of leakiness in replication blockage provided chances for oligonucleotide to mediate repair.

通过检测上述三种抑制剂作用于细胞后不同时间的细胞周期分布，发现thymidine在抑制DNA合成的同时，存在着缓慢的渗漏作用，即染色质DNA上复制叉缓慢移动。

The homologies between the Leishmania isolates in China and the reference strain of WHO were analysed on the basis of determination of a repetitive sequence of Leishmania. The repetitive DNA fragments of the Leishmania isolates were amplified by PCR and their sequences were determined by using the PCR products.

目的 通过分析和比较我国利什曼原虫分离株与相应的利什曼原虫世界卫生组织参照株在一种DNA重复序列上的同源性以考察这一DNA重复序列应用于鉴别我国利什曼原虫的价值。

Results The positive rate of HBVDNA was 56.7%,and the level of HBV DNA was 4.16+0.57( LogE ,copies/ml)in saliva.

结果60例慢性HBV 感染者中唾液HBV DNA 阳性34例，平均HBV DNA 含量为4.16±0.57拷贝数/ml 的对数

To prove that the cloned DNA fragment can express tryptopanase,a new plasmid pET28C-TnaA , in which the cloned DNA fragment was located downstream of T7 promoter on pET28c was constructed and transformed into host BL21(DE3),a BL21 lysogen of bacteriophage DE3 in which the only promoter known to direct transcription of the T7 RNA polymerase gene is the lacuvS promoter ,which is inducible by IPTG.

为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个DNA片段克隆到PET28c质粒的BamHI和HindⅢ位点上，使该片段受T7 RNA聚合酶的启动子控制，然后转化噬菌体DE3的溶源菌BL21(DE3)。

And the repression of NO-induced cell death with Ppbi-1 overexpression in transgenic lines was apparent even at SNP concentrations up to 1mM.In addition work,we found that most of suspension cells treated with 0.5mM SNP can be stained by Sytox green,some PCD morphologic characteristics such as chromatin condensation and margination can be observed.DNA Ladder was also detected with these cells.As to the control and the cell treated with 0.5mMSNP+20μM Est,the above-mentioned characteristics can\'t be detected.

对0.5mM SNP和0.5mM SNP+20μM Est处理的飞廉转基因细胞培养三天后用特异性核染料sytox染色，0.5mMSNP单独处理的细胞大部分核被染色，而且细胞核出现了核凝聚、以及染色质边集等凋亡细胞的特征；提取细胞DNA电泳分析，观察到了DNA LADDER，这说明，该细胞可能已经发生凋亡，而Est诱导Ppbi-1基因表达的细胞均未出现这些凋亡特征。

After using the methenamine silver-DNA staining,15 cases of eariy molluscum contagiosumtissues were examined by electron microscopy.

用DNA银染技术显示了传染性软疣病毒形态发生发育及其过程中DNA的变化。

PCR is very sensitive, allowing a single molecule of target DNA to be amplified to microgram amounts of DNA.

PCR十分敏感，可以从一个DNA分子扩增到微克的量。

DNA microinjection is the most popular and reliable method of producing transgenic animals.

DNA显微注射是生产转基因动物最可靠和最常使用的一种方法，外源DNA的纯度对显微注射的成功起着至关重要的作用。

We establish a microtechnique developed from cytochrome C spreading methods allowing the detection of minimal amounts of DNA through a calibrated composition of buffer、denaturing agents and cytochrome C in an extremely small volume of spreading mixture.

将变性剂脲、乙二醛、甲酰胺引入展开过程，我们在国内首次建立了可检测1×10〓--2×10〓ug以下量DNA样本的微量展层技术，能较好地展开dsDNA及ssDNA分子，DNA形态及长度与经典技术所制样本相似。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。