查询词典 DNA

A quick DNA extraction technique which was fitting for maize variety identification and establishing DNA fingerprinting pool was explored ,and the technique was well applied in the following tissues for DNA extraction : embryo, endosperm; radicel, coleoptile , hypocotyl from germinated 1～3 days material .

本实验探索了适用于玉米品种鉴定及DNA指纹库构建的玉米品种DNA快速煮沸法，用快速煮沸法提取了玉米种子胚、胚乳；水培初生根、侧根；种子出苗后的初生根、侧根、胚芽鞘、胚轴、叶片等组织DNA，结果表明，种子胚、胚乳组织；水培2～4d水培初生根、侧根组织；出苗1～5d的胚芽鞘、胚轴组织用快速煮沸法提取DNA效果较好。

These results may help to exposit the predilection of DNA bases and the flexibility of DNA strands. Thirdly, by changing parameters, i.e. interactive coefficients, elastic coefficients and characteristic frequencies we simulated one of the nonlinear solitary model of DNA via Runge Kutta method to obtain some direct descriptions of the transportation of soliton in DNA double strands.

第三，选择了一种DNA的非线性孤子传递模型，通过选用不同相互作用常数，不同的弹性系数和不同的特征频率，利用龙格库塔法对该模型进行了计算机模拟，对孤子能量在DNA中的传递给予了直观的描述，并从理论上估计出孤子在DNA中传递的速度约为250m/s。

The interaction of chemical molecules and biological ones, especially sequence specific recognition and site-specific cleavage of DNA have many applications such as in DNA sequence determinations, chromosome analyses, gene therapeutics, and recombinant DNA manipulation. So it is significant to develop artificial enzymes for the scission of nucleic acid with high activity and selectivity at normal condition.

化学小分子与生物大分子的相互作用，尤其是人工核酸定位切断试剂对DNA的切断在DNA序列测定、染色体分析、基因治疗以及DNA重组方面有着广泛的用途，因此进行人工合成核酸定位切断试剂及其与DNA的相互作用的研究具有重要的理论意义和应用价值。

Nano-Fe_3O_4 can induce DNA single-strand breaks without significancy and can not induce micronuclei formation and percentage of apoptosis. Nano-Fe_3O_4 can aggravate the effect of CdCl2 on DNA single-strand breaks and chromosome damage in HepG2 cells. They showed synergistic effects on DNA single-strand breaks after the data were statistic analyzed.

结果发现CdCl2能引起DNA断裂损伤、微核率的增加和细胞凋亡率的增加；nano-Fe_3O_4能引起DNA断裂损伤增加，但无显著意义，未引起微核率和细胞凋亡率的增加；nano-Fe_3O_4能加重CdCl2的对细胞DNA断裂损伤和染色体损伤，且二者联合作用对DNA断裂损伤的结果经统计分析认为有协同作用。

The DNA contents of the majority of multiple pengze crucian carp studied were close to that of tetraploidy, and the DNA contents of the minority of multiple pengze crucian carp were between that of triploid and tetraploidy.

结果显示，复合鲫的DNA含量明显多于母本种彭泽鲫的DNA含量，且所测14尾中多数个体的DNA含量超过或接近四倍体的DNA含量，少数个体的DNA含量介于三倍体和四倍体之间。

The results demonstrate that ACM-A is capable of intercalating between the base pairs of DNA double helix, the π-π electronic overlapping between π-electron of ACM-A and DNA stabilizes the ACM-DNA adduct, and through electrostatic interaction, the trisaccharide interacts with the minor groove of DNA owing to C(3') amino group.

实验结果表明，ACM-A主要以其蒽环平面嵌入DNA双螺旋碱基对之间，发生-等电子间相互共轭，同时药物糖基上C3'位的-N(CH3)2基团带正电荷，易与带负电荷的磷酸骨架产生静电吸引，从而发生沟槽作用，嵌入DNA的小沟区，这是二者之间的主要结合模式。并且，DNA与ACM-A药物原型的亲和力要强于还原后的药物代谢产物。

By using λDNA labled with 32 P as probe,unlabled λDNA as positive check,dot blottings of nuclear DNA and chloroplast DNA of receptor 814527,D type sterile line and its hybrid F 1 with Lumai 14 were carried out.

用32 P标记的λDNA作为探针，对受体 81 45 2 7、D型不育系及其与鲁麦 1 4号的杂种F1 核DNA和叶绿体DNA进行点杂交。

The above method can be used as a general method of determining molecular weight distribution of polymers.4.Solid-state ~1H wide-line NMR and ~(31)p magic angle spinningNMR were applied to a series of PEI/DNA complex samples with different PEI molecular weights and nitrogen/phosphatemolar ratios,in order to disclose the influence of various structural factors on the interactions and morphology.The results of ~1H NMR spectra showed that the linewidth of the PEI signal was greatly broadened with increasing the N/P ratio.Meanwhile,in the ~(31)p M_AS experiment,the signal was found to shift upfield with the increase of the N/P ratio.Both ~1H and ~(31)P NMR experiments demonstrated that the interaction between two constituents and subsequently the morphology of the complexes vary with the N/P ratio.

首次将固体核磁共振应用到聚合物/DNA复合物体系，观察复合物体系中不同N/P比对其相互作用和相结构的影响，研究表明N/P比的变化对聚乙烯亚胺/DNA基因递送体系分子水平上相互作用有着很大的影响；另外，还发现不同分子量的聚乙烯亚胺聚合物载体在N/P比为3的情况下对复合物中DNA的微观尺度没有明显影响；通过氢的自旋扩散实验，首次测得在PEI/DNA复合物体系中DNA相尺寸的大小，并发现在体系中N/P比达到3以上的复合物中DNA和PEI达到了分子水平上的相容。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

However, analysis of DNA methylation level indicated that the total DNA methylation levels were not significantly different between diploid and autotetraploid. Autotriploid showed a trend of low DNA methylation level. It implied that the alterations of DNA methylation levels were not linear with the autopolyploidy level in watermelon.

有趣地是，二倍体与四倍体西瓜相比DNA甲基化水准却未发生大规模的调整，而三倍体西瓜显示出较为明显的低DNA甲基化水准趋势，表明西瓜同源多倍化过程中其基因组DNA甲基化水准变化与其倍性不成线性关系。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。