查询词典 DNA

It was not cross- reaction with Entamoeba histolytica and Schistosoma japonica.It is a simply and better method to extract the DNA with lysine boiling.

结果 经扩增于500bp、670bp、1200bp出现特异性片段，与原选择的编码基因片段大小相同，此特异片段与溶组织内阿米巴、日本血吸虫DNA无交叉反应，不同的DNA提取法结果显示：用裂解煮沸法同样可从仅含1条旋毛虫幼虫的25μl正常小鼠血浆中扩增出特异性片段。

Some sperm with damaged DNA may escape from the sperm surveillance mechanism and transmit the damage to the offspring.So research on the damage to sperm DNA has become one of the hot spots in reproductive medicine.

替代组蛋白成为精核蛋白的鱼精蛋白和大量的二硫键使精子染色体高度压缩，这种特化的结构可以保护精子DNA免受伤害，因此成熟精子对各种致病因素有较强的抵抗力。

Euchromatin Network was created by John H. Frenster of USA. It is designed to help researchers and others interested in the latest developments in our studies of this most active part of the genome within the cell nucleus including Ultrastructural Probes of Active DNA Sites, and the RNA Activators of DNA.

该网络由美国人约翰·H·弗兰斯特创建，旨在为动植物染色质研究人员及对该领域感兴趣者提供有关细胞核中基因组研究的最新信息，包括激活位点的超级探针、DNA的RNA激活子。

There is increasing evidence that sperm DNA anomalies may lead to infertility,abortion,stillbirth,fathead and chromosomal diseases.

传统的精液检查往往不能找到精子DNA完整性异常患者的不育原因，因此对这部分患者进行精子DNA完整性检查具有更重要的意义。

Objective:To develop a real-time florescence quantitative polymerase chain reaction method for quantitation of circulating DNA in plasma and explore its value in the diagnosis and prognosis of breast cancer.

目的：建立一种荧光定量PCR方法用于血浆循环DNA定量，并讨论血浆循环DNA检测在乳腺癌诊断及预后中的意义。

The analysis of genetic sign of mitochondrial DNA is of great value in forensic medicine practice.

线粒体DNA(mitochondrial DNA，mtDNA)遗传标记的检测在法医学实践中有着重要的价值。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

Results A 357 bp DNA band was seen in the 2% agarose gel after the PCR product was electrophoresed. After the 357 bp DNA band was recovered and ligated into pMD-18T, human transforming growth factor-beta 3 encoding gene acquired through four-cycle PCR was confirmed by sequencing.

结果：4次PCR后，经2％的琼脂糖凝胶电泳可见一357 bp的条带，将电泳产物回收，连接入pMD-18T载体，经测序分析证实，所获得DNA片段为人转化生长因子β3的编码基因。

In accordance with the fact that 5-brom-odeoxyuridinecan incorporate period-ically into the nuclear DNA to substitutethe thymidine in DNA,we devoloped a newbasic fuchsin staining method in which weused BrdU as a chromosomal marker in in situnick translation of chromosomes.

根据5—溴脱氧尿嘧啶核苷可周期性替代DNA链中的胸腺嘧啶核苷而显示不同细胞周期染色体的特性，我们用BrdU作为染色体的标记，首次建立了一种硷性复红染色的染色体原位切口移位的新方法。

Methods: Total of 37 serum samples from patients with fulminant hepatitis were detected for TTV DNA by nested PCR methods.

设计TTV基因的特异性引物，采用巢式PCR法对37例重型肝炎进行血清中TTV DNA的检测，并对TTV DNA阳性病例进行临床资料分析。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。