查询词典 DNA

The prolifertion of DNA diploidy and DNA abnormal ploidy is equally in nasopharyngeal.

不同DNA干系倍体鼻咽癌的细胞增殖活性相同。

Methods: Cells which belonged to the G1-phase were sorted by Multiparameter flow cytometry according DNA diploidy in asynchronous MOLT-4 cell lines, cells were synchronized at the G1-S boundary by thymidine, and western blot analysis and multiparameter analysis of DNA versus protein content was performed to examine cyclin A、B1、D〓、Eexpression in sorted G1-phase MOLT-4 cells and synchronized by double thymidine block in G1/S phase.

以MOLT-4细胞为模型，应用流式细胞术分选出非同步化生长的G1/S转换期起始处的G1期细胞，用"双thymidine阻滞法"将细胞同步在G1/S转换期起始处的G1期，采用DNA/cyclins双参数分析法及免疫印记法，检测同步化及非同步化G1期细胞中cyclinA、B1、D〓、E的表达。

Metallic ion effects on DNA stretching were investigated. It is found that the effects of monovalent and divalent cations were significantly different.

研究了金属离子对DNA拉伸的影响，发现一价金属离子和二价金属离子对DNA的影响有显著的差别。

The influence of DNA damage induced by γ ray at different concentration of DNA under different dose rate was investigated.

利用γ射线在不同的剂量率下对不同浓度的质粒DNA pUC19水溶液进行了辐照，利用凝胶电泳对辐照后的样品进行分析。

But this molecular model shows how some proteins form loops in DNA when they chemically attach, or bind, at separate sites to the double-helical molecule that carries life's genetic blueprint.

就像槲寄生包绕在柔软的甘蔗蔓藤上，分子模型展示了某些蛋白在DNA 如何形成环状，当它们用化学键贴或者绑在携带生命遗传蓝图的双螺旋DNA分子。

The reason is that the energy deposited by heavy ions can directly or indirectly migrate along DNA strands, and can firstly break the bonds of highest electron affinity and lowest ionization potential.

重离子辐照沉积的能量可以直接或间接地沿DNA链迁移，从而使得DNA分子上相对较弱或亲电性较强的化学键优先断裂。

The interactions of dsDNA and ssDNA with two osmium complexes have been studied by surface electrochemical methods based on DNA modified electrode. It is found that both osmium complexes bond DNA with electrostatic interaction. And some thermodynamics and kinetics constants, such as bindind constant , ratio of binding constants K〓/K〓, limiting ratio K〓°/K〓°at zero ionic strength (μ＝0), free energy ΔG〓, dissociation rate constant k and binding site size s have been obtained.

研究发现两种锇配合物都是通过静电作用与DNA结合，并计算得到联吡啶锇和二氯菲咯啉锇与dsDNA和ssDNA相互作用的结合常数、结合常数比K〓／K〓、μ＝0时极限结合常数比K〓°／K〓°、结合自由能ΔG〓、解离速度常数k和结合位点数s等多个热力学和动力学参数。

Enterococcus faecalis DNA can be detected in encephalon, liver and blood spleen in artificial infectious mouse at 2h.

结果发现感染2h后用PCR方法即可从脑、肝和心血检测到粪肠球菌DNA.6h后不仅能检测到肠球菌DNA，而且从上述组织中分离到肠球菌。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

A PCR product was amplified using RT-PCR method from total RNA from the abdomens of R strain using the degerated primer. The PCR product was purificated and cloned. The plasmid is pBluescript Ⅱ KS. Recombined plasmid DNA was identified by endoenzyme. The results indicated there were different P450 genes in the PCR product.

运用这对简并引物首次从德国小蠊抗拟除虫菊酯的品系R中扩增出的特异性的DNA片段，经纯化、连接和转化，重组至pBS质粒，筛选阳性克隆，提取重组质粒的DNA，并经初步的酶切鉴定，发现存在着不同的CYP4基因。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。