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Possible fluorescence enhancement mechanism is also studied. Chapter 3, a novel assay of DNA is proposed based on the measurement of enhanced resonance light-scattering signals resulting from the interaction of nano-CdS-Lcysteine with DNA.

3第三章,用L-半胱氨酸作为修饰剂合成了功能化的CdS量子点,并利用DNA的加入对其共振光散射光谱的影响建立了超灵敏测定DNA的方法。

The long specific DNA probes were synthesized by polymerase chain reaction in the highly specific gene sequences of above 10 bacteria. The species-specific probes synthesized were inoculated on a nitrocellulose filter and hybridized with template DNA labeled with long-arm photosensitive biotin. The signal of hybridization reaction was observed to detect which bacteria infected. The sensitivity and specificity of the method were studied.

在这10种病原菌高度特异的基因序列内利用聚合酶链反应合成上述10种细菌特异的长链DNA探针,并将合成的特异探针固定在硝酸纤维素膜上,与用长臂光敏生物素标记后的模板DNA进行斑点杂交反应,通过观察杂交信号判断存在何种细菌感染,同时研究该方法的敏感性和特异性。2。

This study is try to explore if the mitochondrial DNA 889bp and 3895bp deletions in human lym- phoblastoid cell line could be induced by ionizing radiation.

研究发现,用于扩增线粒体DNA 889bp的引物在扩增出目的DNA片段的同时,也可扩增出未发生缺失的DNA片段。

The innate cel DNA damage could be inhibited by Vit Cand Vit C could also inhibit the lym phocyte DNA 哪in mice induced by organic extracts from water.

维生素C能够降低自发的细胞DNA损伤并对水中有机物致小鼠外周血淋巴细胞DNA的损伤具有抑制作用

The results presented in this paper indicated that sodium salt of 3,7-bishemis uccinate of 7-hydroxycholesterol (RS 634) at the dose of 45mg/kg/day (×7,ip) could increase DNA, RNA and protein syntheses in bone marrow cells and protein synthesis in liver,spleen and serum, but no markedly effects on DNA and RNA synthesis in liver and spleen of mice bearing EAC were observed, In the subacute test on rats RS 034 (400mg/kg/day, x30 ip) showed no evident effects on the number of RBC, WBC and the percentage of lym...

本文报告的结果表明7β-羟基胆固醇双琥珀酸单酯钠(RS 024)在有效抗肿瘤剂量下(45mg/kg/day,×7ip)对荷瘤小鼠骨髓细胞DNA、RNA和蛋白质合成有促进作用,对肝、脾的DNA、RNA合成无显著影响,但能促进肝,脾中及血清蛋白质合成。按400mg/kg/day连续给药30天,对大鼠红细胞、白细胞数及淋巴细胞百分率无明显影响。提示该药在有效抗癌剂量下毒性较小,并在一定程度上对免疫功能有增强作用。

U.S. and Japanese scientists medaka fish through the analysis of deoxyribonucleic acid all of the information and found that the structure of DNA nucleosome affect DNA variation, thus affecting the biological evolution.

美日两国科学家通过分析青鳉鱼脱氧核糖核酸的全部信息,发现DNA核小体结构影响着DNA变异,从而影响生物进化。

Both UV-A and UV-B irradiation induced DNA damage in mesophylls. The level of DNA damage was much higher in younger than mature leaves, which indicates that young leaves are more sensitive to UV-B radiation than mature leaves.

彗星检测结果表明,九里香叶片DNA的损伤程度与UV-B辐射的剂量呈正相关;在相同UV-B辐射剂量下,九里香幼嫩叶片比成熟叶片的DNA损伤量大,表明其幼嫩叶片对UV-B辐射的敏感性比成熟叶片高。

This dissertation aims at finding anticancer and antibacterial polynuclear complexes targeting DNA with high activity and low toxicity. Sixteen compounds were synthesized, and characterized by means of X-ray single crystal diffraction, elemental analysis, molar conductivity and IR, and the influencing factors on structures of the compounds have been investigated. The actions of these compounds to DNA were also investigated by spectral, electrochemical and hydromechanical methods.

本论文以DNA为靶点,以寻找新结构类型的高效、低毒的抗癌、抗菌多核配合物为目标,从分子设计出发,共合成得到了16个化合物的单晶结构,对其进行了结构表征,探讨了结构影响因素,并研究了其超分子结构;采用紫外-可见吸收光谱法、荧光光谱法、电化学和粘度法,从分子水平上研究了所得配合物与DNA的相互作用;在细胞水平上,研究了草酰胺桥联双核配合物的抗菌和抗肿瘤活性。

DNA damage by mutagens has very serious consequences for DNA replication.

由诱变剂引起的 DNA 损伤会在 DNA 复制时带来严重的后果。

Under the excitation of 488 nm light beam of argon ion laser source, single suprahelical species could be observed with the aid of a common microscope due to the strong scattered light emitted by the suprahelical species. By capturing the RLS images of the single suprahelical species with a cooled charge coupled device camera, and analyzing the RLS data, herein an RLS imaging method of DNA was proposed based on the linear relationship between the counts of suprahelical species in the detection focus plane and the concentration of DNA in nanograms.

在488nm 氩离子激光光源的激发下,单个超螺旋聚集体的散射光可以通过普通显微镜观察到,用冷却型电荷耦合器对此单个粒子的RLS信号进行成像和分析,发现在聚焦平面上检测到的超螺旋粒子的个数与溶液中纳克级的DNA浓度成正比,基于此建立了一种测定DNA的方法。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。