查询词典 DNA

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

Results The DNA contents of different categories of samples were as followings:suck pipes(0.72-116.78 ng),cup edges(2.15-142.5 ng),mouths of drin...

结果从25根饮料吸管上提取的DNA量在0.72～116.7.8ng，16个水杯杯缘提取的DNA量在2.15-142.5ng，31个饮料瓶口提取的DNA量在1～34.65ng，10根筷子上提取的DNA量在3.35～26.6ng，12个果核中提取的DNA量在0.294～21.4ng，6份吃剩的骨头中提取的DNA量在0.88～5.88ng.100份检材性别及9个以上STR位点分型成功率平均为59.38%。

Nuclear DNA content analysis revealed that hybrid plants had different nuclear DNA content:higher,lower than the parental sum or equal to it.Contrast to the lower and equal hybrids,bigger amount of hybrids with higher DNA content had abnormal leaf,bad growth vigour and poor fertility.

结果表明：杂种形态变异广泛，多数呈中间类型，少数呈偏亲性状；杂种细胞DNA含量不一致，呈现大于、等于、小于双亲细胞DNA含量之和的类型；DNA含量大于双亲之和的杂种与DNA含量等于或小于双亲之和的杂种相比，有较高比例的材料叶型出现畸形和植株生长势弱，育性也更差。

They are often morphologically distinct from A chromosomes, being smallerand more highly heterochromatic in most cases. B chromosomes do not pair with Achromosomes, and they are inherited in a non-Mendelian way, exhibiting meiotic andmitotic instability and nondisjunction. However, B chromosome DNA is quiteidentical to the corresponding sequence on the A chromosome complement. So far afew B chromosome specific DNA sequence have been identified. Specific DNAsequences on B chromosome have been the attractive research area on Bchromosomes.

现已在千余种植物和近三百种动物中被发现。B染色体与物种中正常染色体不同：独立于整倍体基因组之外，减数分裂时不与A染色体发生联会和配对，细胞分裂后期不分离，非孟德尔遗传，富含异染色质，不含对宿主主要性状有影响的基因等。B染色体DNA的分子组成既与A染色体极为相似，具有A染色体DNA分子组成的一般特征：富含重复序列和转座成分，染色体三大功能组件DNA高度同源；又与之相区别，含有B染色体特异的DNA序列，这些DNA序列可以为探讨B染色体的起源和进化提供有价值的信息。

In this paper the thymus DNA of calf and the pBs plasmid are the main objects of our studies in ultraweak luminescence. By means of PIAS(Photon-counting image acquisition), ultraweak luminescence detector and Lambda Bio 40 of UV/VIS spectrophotometer, we studied the UL of DNA induced by the three oxygen free radicals: singlet oxygen Oa, hydroxy radical and superoxide anion(O2 ). We found that the OH can greatly increase the UL intensity of DNA.

本论文以小牛胸腺DNA和质粒pBs为实验材料，利用光子图像探测系统(Photon-counting image acquistion，PIAS)、微弱发光测量仪和UV／VIS分光光度计和生物统计学的成组比较法原理对单线态氧(~1O_2)、羟自由基、超氧阴离子(O_2~-)三种氧自由基诱导DNA超弱发光的变化过程进行了研究，结果发现这三种氧自由基中·OH能诱导DNA的超弱发光发生显著的增强变化，而其他两种氧自由基对DNA的超弱发光并无显著诱导作用。

The signals look like noncontinuous beads on the DNA fiber,which consist of multi-copy and arrange tandemly.Fiber-FISH results with 45S rDNA probe showed that an average signal length is about 3～11μm in many tandem copy sequence(measure number,n=8),so we estimated the size of each copy to be approximately 11～30kb in cotton genome.There are dual signals on each end of the mitosis metaphase chromosomes and pachytene chromosomes hybridized with telomere DNA probe.The signals also look like non-continuous beads on the DNA fiber,the length is about 1～9μm,so we estimated the size is about 4～27kb.The signals almost covered the whole mitosis metaphase chromosomes and pachytene chromosomes of G.arboretum Shixiya 1 hybridized with gDNA probe,the euchromatin zone and heterochromatin zone were identified clearly on pachytene chromosomes,and the signals also look like non-continuous beads.Two BAC clones 150-D-24 and 182-F-9 in DNA BAC library of Pima90 were selected as probe to hybridize with mitosis metaphase chromosomes,pachytene chromosomes and DNA fiber of G.raimondii.

棉花FISH技术系统的应用研究。45S rDNA在亚洲棉石系亚1号中期和粗线期染色体上有两对大的信号，在DNA纤维上信号为非连续的念珠状，多个拷贝串联排列，每个拷贝长度大约在3～11μm，推测每个拷贝的实际长度为11～30kb；端粒序列在亚洲棉石系亚1号中期和粗线期染色体端部都有双点信号，在DNA纤维上信号也为非连续的念珠状，不同染色体上信号长度大约在1～9μm不等，推测实际长度为4～27kb；gDNA信号几乎布满整个亚洲棉石系亚1号中期和粗线期染色体，在粗线期染色体上能明显区分出常染色质区和异染色质区，DNA纤维上的信号均为非连续的念珠状。

The interactions between phenol,m-cresol or catechol with DNA sensor were analyzed, results indicated that the threephenols damaged DNA.The direct oxidation peaks of DNA were decreased linearly withthe phenols concemtrations.R%D_(50) values showed that the m-cresol damnified DNA mosteasily.

研究了苯酚、间甲酚和邻苯二酚与DNA的作用，结果表明其对DNA有损伤作用，且存在剂量效应关系，一定浓度范围内，DNA的峰电流与酚的浓度呈线性下降关系。R％D_(50)值分析结果表明这三种酚污染物中间甲酚最容易损伤DNA。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

Results showed that hypochromism and red, shifted KAE absorption spectra were observed in the presence of DNA, and that synchronous、 fluorescence of DNA-NR was quenched with addition of KAE, indicating that a strong competition for DNA binding between KAE and NR existed, in addition, the relative viscosity and the value of melting temperature of DNA increased in the presence of KAE.

结果表明：加入DNA后，山柰酚的吸收光谱呈现减色和红移现象；山柰酚的加入使得DNA-NR同步荧光强度猝灭，表明山票酚对NR与DNA的结合为竞争性抑制；山柰酚的加入使得DNA的粘度增大，热变性温度升高。

The results showed that truncations of T-DNA borders, insertions of filler DNA and the formation of inverted and direct repeat occurred during T-DNA integration. From the cleavage site of the T-DNA borders, no truncations occurred for nine left borders, most of the left borders deleted less than 35bp and most of the right borders truncated to 21bp.

分析了233个T-DNA左边界和190个T-DNA右边界与水稻基因组交接位点的序列，结果表明T-DNA在整合过程中不仅伴随着左右边界序列的缺失及&filler DNA&的插入，而且还以单拷贝或多拷贝的形式插入在基因组中的同一个位点。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。