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Objective To study the immunogenicity of the PrM-E gene DNA of Chinese dengue 2 virus 43 strain and the effect of unmethylated CpG motif in the DNA immunization.

目的 观察我国登革2型病毒43株PrM-E基因的DNA免疫原性及非甲基化细菌性CpG对DNA免疫效果的影响。

Objective To study the immunogenicity of the PrM-E gene DNA of Chinese dengue 2 virus 43 strain and the effect of unmethylated CpG motif in the DNA immunization.

目的 观察我国登革2型心理病毒43株PrM-E基因的DNA免疫原性及非甲基化细菌性CpG对DNA免疫效果的影响。

Immunostimulatory sequence (155), unmethylated CpG dinucleotides in particular base contexts, are relatively common in bacterial DNA but rare in vertebrate DNA, and can be detected as an infectious danger signal by the vertebrate innate immune system.

DNA免疫激活序列(immunostimulator sequence,ISS)是发现于细菌DNA 中的一些以未甲基化的 CpG二核苷酸对为核心的具有免疫激活功能的核苷酸序列。

The recombinant plasmid DNA of PrM-E gene but not unmethylated CpG was capable of inducting antibodies against D2-43 virus.

我国登革2型病毒PrM-E基因重组质粒DNA具有一定的免疫原性。在本实验条件下,含非甲基化细菌性CpG的pUC19质粒DNA,对PrM-E基因的DNA免疫效果没有促进作用。

Genomic DNA of positive transformed plants; 6, 7: Genomic DNA of negative transformed plants; 9: genomic DNA of untransformed plants; 10: Positive control of 3G1MHpCAMBIA2301; 11: NTC; 12: DL 2000 marker.

2.2 转基因拟南芥的筛选选择培育后获得转基因拟南芥植株。从其叶片中提取微量DNA, PCR鉴定,可扩增出750 bp左右片段(图3),表明3G1MHpCAMBIA2301基因已整合到拟南芥染色体中。

Venturia inaequalis ; DNA extraction method ; DNA extraction rate

苹果黑星病菌; DNA提取方法; DNA得率

By using random amplified polymorphic DNA technique, this paper studied the effects of Cd stress on DNA polymorphism in root tip of Vicia faba seedling s.

采用随机扩增多态性DNA技术检测镉胁迫对蚕豆幼苗根尖DNA多态性的影响。

In the bacteriophage phi29, a virus-encoded RNA is required for the packaging of its genomic DNA. Six pRNAs form a hexamer through pRNA/pRNA interaction to drive the DNA translocation motor, and ATP as the energy resource.

在噬菌体phi29中,基因组DNA的包装需要由病毒基因组编码的pRNA参与,6个pRNA分子通过由pRNA分子间相互作用形成的六聚体来启动DNA转运马达,这个过程由ATP提供能量。

Genomic DNA in eukaryotic cell is organized into nucleosome array and further packaged into higher-order structures. Each nucleosome is composed of a stretch of approximately 147bp DNA wrapping around a histone octamer, and neighbored nucleosomes are connected by linker DNA with length varies from 20bp to 100 bp.

中文摘要真核生物的基因组DNA能够与组蛋白形成核小体结构,这些核小体结构在基因组上线形排列,并进一步折叠成更加紧密和复杂的高级结构。

Bacteria genomic DNA was extracted to do polymerase chain reaction to amplify specific DNA and then to assess coden sequence.

萃取细菌DNA后利用聚合酶连锁反应将细菌特定的DNA片段增幅,定序比对标准序列判定基因型。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。