查询词典 DNA

The interaction of S-metolachlor with calf thymus DNA was investigated using UV absorption spectra, fluorescence spectra, thermal denaturation and viscometry of DNA.

应用紫外光谱、荧光光谱、DNA热变性法以及粘度法研究了S-异丙甲草胺与小牛胸腺DNA的相互作用。

A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding small molecules, such as the gel mobility shift assay,~1H NMR, electrochemistry analysis, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry.

目前有很多方法应用于实验室研究DNA与其他物质的相互作用，比如：凝胶电泳分析，核磁共振，电化学分析、DNA足印分析，荧光分析，紫外可见光谱分析以及黏度分析等。

The single micronucleus and multi-micronucleus were observed at the interphase. The chromosome aberrational cells including chromosome fragment, lagging chromosome, chromosome bridge and circular chromosome were found during the mitosis. RAPD analysis of seedling genomic DNA variation in M 2 generation of three mutants showed their DNA sequences had changed. The result confirmed that the implantation of 7Li +3 ions could induce genetic mutation in wheat.

对幼苗根尖细胞学研究观察，获得单桥、双桥、桥断裂、落后染色体、三级、断片和微核等畸变类型；对已获得的小麦突变株，分别进行M2 代DNA分子的RAPD分析，结果发现DNA均发生变异，证明7Li+ 3 注入通过内靶核反应能够诱发小麦在分子水平上产生遗传变异。

OBJECTIVE To detect the effects of DNA from actinobacillus actinomycetemcomitans associated with periodontal disease for the immune response to other bacterial antigens in the oral cavity, and to study the feasibility of DNA from actinobacillus actinomycetemcomitans associated with periodontal disease as adjuvant of oral vaccine.

［目的］测定牙周病相关细菌伴放线放线杆菌DNA对口腔内其他细菌性抗原的免疫应答的影响，并探讨牙周病相关细菌伴放线放线杆菌DNA作为口腔疫苗佐剂的可行性。

Genetic cluster analysis is similar based on the results of morphological and DNA markers, the five populations could be divided into three groups. But there are different groups sort of individual locations, reflecting the similarities and difference about the analysis of the relationship between population with the two kinds of markers, It could be concluded that there was coupling and relevance to some extent in evaluating genetic diversity with the same experiment sampling by morphological and SSR marker variation. However, there was also differences. In research, the two can complement each other.8. Identification of Genetic Relationship of Amygdalus plants by SSRThe genetic relationship among 55 Amygdalus plants collections, belonging to 6 species and collected from both homeland and abroad were identified via SSR molecular marker technique.

新疆野扁桃遗传多样性不同研究分析方法结果的比较与评价表型与DNA二种水平的遗传标记方法揭示的遗传多样性水平有差异，表型性状比DNA分子标记具有更大的变异性；其受到环境的影响较大，也即对环境更敏感。2种水平的遗传标记聚类结果将野扁桃5个不同分布群体均可分为3个大类群，但是出现有个别不同的群体排序位置，反映了2种标记在分析种群间关系上的异同性，从本研究结果来看，说明表型与DNA标记有一定的耦合性和相关性，但也存在差异，在研究上可以互为补充。8。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

To explore the feasibility of the mitochondrial DNA as transgenic vector, we translated the mtDNAs of the Antheraea pernyi, Diaphania pyloalis Walker and the fish to the silkworm. Through microscopic examination and mtDNA polymorphism analysis, we tested the maintenance of the foreign mtDNA in the silkworm and investigated the influence of the foreign mtDNA to the physiological behavior.

为了探索线粒体DNA作家蚕转基因载体的可行性，本实验将柞蚕、桑螟和鱼的线粒体DNA转入家蚕体内，通过着色镜检、线粒体DNA多态性分析检测了外源线粒体在家蚕细胞中的存留情况，并调查了外源线粒体对家蚕生长的影响。

In order to investigate new methods to the detection of Mycobacterium tuberculosis and diagnostic value of FQ-PCR in the detection of DNA in Mycobacterium tuberculosis, FQ-PCR was used to detect DNA of Mycobacterium tuberculosis in body fluid from 68 patients with tuberculosis and 68 patients without tuberculosis and it was also compared with the two methods of smear acid-fast stainning and antituberculotic antibody.

为探讨结核分枝杆菌检测的新途径及其FQ-PCR技术在结核分枝杆菌DNA诊断中的实用价值，运用FQ-PCR技术对68例结核病患者和68例非结核病患者体液进行结核分枝杆菌DNA测定，并与涂片抗酸染色及ELISA—抗结核抗体法进行比较。

The electrophoresis of DNA showed that lower dose (20Gy) irradiation made little influence on the DNA of armillaria,while higher dose(200Gy) irradiation resulted in a lot of breaks of DNA strands and these breaks mostly appeared in the 6 hours after irradiation and could be self\|repaired gradually.

DNA断裂主要发生在辐照后 6h之内，以后能自动修复

Results There existed the correlation between atlE gene expression and the quantity of extracellular DNA re leasing into the medium, and DNase Ⅰ affected biofilm formation and reduced initial adherent capacity of S.

结果 表皮葡萄球菌atlE基因的表达与胞外DNA的释放量有相关性；DNA酶能影响未成熟生物膜且能降低起始黏附能力；ΔatlE菌株胞外DNA的释放减少，起始黏附能力明显降低。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。