查询词典 DNA

The fluorescence emission peak of inclusion complex shifted toward short waves and fluorescence intensities decreased in the presence of DNA. The quenching constant was calculated to be 6.12×10~4L mol~(-1) by Stern-Volmer method. The quenching mechanism was studied. The interaction ofβ-CD-TH inclusion complex and thionine with DNA were compared.

荧光光谱实验的结果是DNA对硫堇包合物的荧光峰有蓝移和猝灭效应，猝灭常数Ksv为6.12×10~4L mol~(-1)；探讨了其荧光猝灭机理，比较了硫堇包合物和游离态硫堇与DNA作用程度的大小。

Furthermore, besides considering changes in DNA methylation as mechanisms of disease, the role of epigenetics in general and DNA methylation in particular in transgenerational carcinogenesis, in memory formation and behavior establishment are brought about as mechanisms based on the cellular memory of gene expression patterns.

而且，除了考虑DNA甲基化改变作为疾病的机制外，表观遗传学一般的及DNA甲基化特别的在隔代致癌中的作用，在记忆形成和行为确立被引发作为基于基因表达模式的细胞记忆的机制。

The most common form of DNA in a cell is in a double helix structure, in which two individual DNA strands twist around each other in a right-handed spiral.

最常见的形式在细胞的DNA是在双螺旋结构，其中两个单独的DNA链捻周围对方用右手螺旋。

To compensate the descent of pH for the heat-up, exalting the Tris-HCl buffer's pH can avoid the abscission of the purine and make the long sequence PCR go on smoothly. The GC-rich template's secondary structure is complex and stable. The organic reagent methylamine contributes to uncoil the secondary structure.The pfu DNA polymerase has more fidelity and thermostability than Taq DNA polymerase.

高GC含量使模板二级结构复杂而稳定，一般热启动难于解开，而变性温度过高、时间过长又不利于DNA聚合酶的活性作用发挥，于是借助能缓释模板二级结构的有机试剂甲胺扩增高GC含量模板。pfu DNA聚合酶比Taq酶有更高的保真复制性能和热稳定性，更有利于高GC含量模板的扩增。

Thus, the mutant amplification products were made of mostly double-stranded DNA, while the wild-type products were made of mostly unconsumed single-stranded DNA.

突变型样品扩增产物中大部分是双链DNA；而野生型样品由于不能被顺利扩增，产物中大部分是单链DNA。

To establish a new method to isolate genomic regulatory sequence using unpurified nuclear extract, here a new strategy was developed based on modified whole genome PCR and electrophoresis mobility shift assay. The principle is as follows. After sonication and ligation with DNA linker, human genomic DNA is amplified and labeled with isotope by whole genome PCR.

为了建立一种利用未经纯化的细胞核粗提物就能从人基因组中快速、大量筛选DNA调节片段的新方法，本研究对原有的全基因组PCR技术进行改进，结合凝胶阻滞实验，建立了筛选DNA调节片段的新方法。

Main points of this paper are as follows:(1) to explain emphatically and to prove the Jukes-Cantor Model, Kimura Model and the relationships of them;(2) to construct and to prove, using EM algorithm, the theorems of estimating best branches of DNA sequences having censored data for Jukes-Cantor Model under the conditions of rooted tree and unrooted tree respectively;(3) to construct and to prove, using EM algorithm, another three theorems of estimating best parameters of DNA sequences having censored data for Kimura Model under the conditions of rooted tree and unrooted tree.

本论文的重点在于解释和证明Jukes-Cantor模型、Kimura模型及两模型之间的关系；运用EM算法构造并证明Jukes-Cantor模型下含缺损数据的DNA序列构建有根树的最佳分枝长度估计定理；运用EM算法构造并证明Kimura模型下含缺损数据的DNA序列构建有根树的最佳参数估计定理。

In SGC-7901 cells, 5-Fu can interfere with the DNA synthesis and result in the reduction of the DNA content. Besides, it can affect the unsaturation degree of the hydrocarbon chains and alter the external environment of guanine and adenine residues in cancer cells.

这说明5-氟尿嘧啶可以影响DNA的复制合成，降低胃癌细胞中DNA的含量，并且影响癌细胞中脂肪酸链的饱和度以及鸟嘌呤和腺嘌呤的外环境，正常细胞拉曼谱的改变说明5-氟尿嘧啶存在一定的副作用。

Objective:in order to compare the curative effects of using lamirudine and thymosin together with using thymosin only to the paients of chronic hepatitis b.methods:we had chosen 27 medical cases of chronic hepatitis b,which were divided into two groups:15 cases asone group,and 12 cases as the other group.let group a take 100 mglamirudine orally,qd,with half a year as a period of treatment.at the same time givethe patients injections of 100 mg thymosin,qd,for a month.to group b was only takenlamirudine.alt,tbil,the indicators of the virosis of hepatitis b (hbsag,hbeag,hbv-dna)were observed as quatos.results: at the end of the treatment, the rates that groupa and group b made hbsag transform into negative were respectively 26.67% and 16.67%(p＞0.05);hbeag:33.33%,8.33%;hbv-dna:53.33%,33.33%(p＞0.05).conclusion:combining thymic peptide with 3tc to treat chronic type b hepatitis, thymic peptide will not only promote b-cell to mature and transformation, but also to keep the subpopulation of t-cell in immune system in balance, which can enhance the reaction of matured t-cell to the antigen or other stimulus.

目的：比较拉米夫定（3tc）与胸腺肽联用和单用拉米夫定治疗慢性乙型肝炎的疗效。方法：选择慢性乙型肝炎患者27例，分为联合组15例和拉米夫定组12例；联合组用拉米夫定100 mg，口服，qd，疗程半年，合并用胸腺肽100 mg，静滴，qd，疗程1个月。拉米夫定组单用拉米夫定。以谷丙转氨酶、血清胆红素、乙肝病毒标志物(hbsag，hbeag，hbv-dna)为观察指标，比较不同治疗方案对指标的影响。结果：治疗结束时联合组与拉米夫定组对慢性乙肝患者血清hbsag阴转率分别是26.67％和16.67％(p＞0.05)；hbeag阴转率分别是33.33％和8.33％(p＞0.05)；hbv-dna阴转率分别是53.33％和33.33％(p＞0.05)。结论：胸腺肽联合3tc治疗慢乙肝，胸腺肽具有促进t淋巴细胞的成熟和转化，并维持免疫系统各t淋巴细胞亚群间的平衡作用，能增强成熟t淋巴细胞对抗原或其他刺激的反应。

A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding small molecules, such as the gel mobility shift assay,~1H NMR, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry.

目前有很多方法应用于实验室研究DNA与其他物质的相互作用，比如：凝胶电泳分析，核磁共振，DNA足印分析，荧光分析，紫外可见光谱分析以及黏度分析等。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。