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The chromosome composition of Yuan Zhong 2, a common wheat-Ag.intermedium partial amphiploid (2n=54), was analyzed with the genomic in situ hybridization,in which the biotinylated total DNA of Ag.intermedium as the probe and the total DNA of common wheat cv.Chinese Spring as blocking DNA were used to hybridize with the chromosome DNA of Yuan Zhong 2 at mitotic metaphase in root tip cells.

为分析普通小麦-天兰冰草部分双二倍体-远中2号(2n=54)的染色体构成，用生物素(biotin-16-dUTP)标记天兰冰草染色体组DNA作为探针，以普通小麦品种中国春染色体组DNA为封闭DNA，与远中2号的有丝分裂中期染色体DNA进行了分子原位杂交。

After repeating 5 times, the result shows that the genome DNA can be extracted from the fresh specimens by the methods ,but fail to be extracted from the preserved specimens with the same methods through electrophoresis.

经 5次重复实验，结果表明，用两种方法均能从新鲜标本中获得较完整的DNA ，且DNA得率较高；而对于无水乙醇保存的标本，提取出的DNA用琼脂糖凝胶电泳检测不出DNA条带，说明标本在保存过程中DNA可能发生了变化，致使提取出的DNA量太少或未分离出DNA 。

Results All of 10 kinds of JFM fibers could induce DNA strand breaks,DNA-DNA interstrand crosslink and inhibit the ability of DNA repair,but all of these effects were lower than that of chrysotile and were different among them.

结果 10种JFM纤维都可引起不同程度的DNA链断裂、DNA-DNA链间交联及抑制DNA损伤后的修复功能。综合分析发现：微细玻璃纤维的遗传毒性最强，硅灰石的遗传毒性最低。

DNA C-values might be one of important indices in the evaluation of angiospermous invasiveness. To test this viewpoint, we compared DNA 1C-values and basic genome sizes in 3,676 angiosperms. The results suggest that:(1) the two nuclear values vary greatly among different groups, e.g., significantly higher in herbs than in trees, in monocots than in dicots, in perennials than in non-perennials;(2) the two nuclear values both have significant effects on plant invasiveness, especially in herbs, dicots, monocots, perennials, non-perennials, diploids, polyploids, Compositae, and Poaceae, while not significant in trees and Fabaceae.

DNA C值与被子植物入侵性密切相关，为考察应用DNA C值评估被子植物入侵性的可靠性和局限性，我们统计分析了全世界范围内3676种被子植物的DNA 1C值（配子未发生DNA复制时的染色体DNA含量）及基因组大小（单个染色体组的DNA 含量），结果表明：1）DNA 1C值和基因组大小在被子植物不同类群中差异极大，例如在草本植物中显著高于木本植物，单子叶植物显著高于双子叶植物，多年生植物显著高于非多年生植物；2）两项指标都与被子植物入侵性显著负相关，尤其是在草本植物、双子叶植物、单子叶植物、多年生植物、非多年生植物、二倍体、多倍体、菊科和禾本科中，然而在木本植物、豆科植物中其与植物入侵性无显著关系。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Relationship between DNA content and evolutionary degree DNA contents of bivalves were analyzed statistically with SPSS software at subclass, order, family and species grade respectively. Significant difference was detected between mean values of two subclasses according to the result of T-test. The DNA content of Heterodonta (3.77pg) was distinctly larger than that of Pteriomorphia (3.14pg). It was concluded based on ANOVA that the general trend of DNA content was to change from lower to higher values associated with evolution at higher taxonomical classification, i.e.

核DNA含量与进化地位的关系运用SPSS统计软件，在亚纲、目、科以及种4个分类等级上对双壳贝类的核DNA含量进行了统计分析。T检验结果显示，两亚纲核DNA含量的平均值有明显差异，异齿亚纲的核DNA含量平均值(3.77pg)显著大于翼形亚纲(3.14pg)，该结果表明，核DNA含量在较高的分类等级，即亚纲上表现出随着进化地位的升高显著增大的趋势。

The prewashed samples and postwashed samples were analyzed by seminal fluid assay.RESULTS:(1) The positive rate of semen HBV DNA was 21.05%(12/57) with 4.7810~4copies/ml on average; the positive rate of serum HBV DNA was 81.82%(45/55 with 2.3310~7copies/ml on average. There is positive rank correlation between semen HBV DNA copies and serum HBV DNA copies;(2 There were 7 positive cases in 21 samples before washing, the 6 cases except one were positive after washing;(3) there arepositive rank correlation between viability、round cells、 abnormal morphology and semen HBV DNA copies.

结果：（1）精液HBV DNA阳性检出率为21.05%（12/57例），平均拷贝数为4.7810~4/ml，血清HBV DNA阳性检出率为81.82%（45/55例），平均拷贝数为2.33(10~7/ml，精液与血清HBV DNA拷贝数等级间存在正相关关系；（2）21份用于洗涤的精液标本中，7份精液HBV DNA阳性，阳性率为33.33%（7/21例），洗涤后仍有6份标本阳性；（3）精液常规分析显示精子活力、圆细胞数、精子畸形率与精液中HBV DNA载量之间存在正相关关系。

All these results indicate that the DNA variation process during the occurring process of adenocarcinoma may be: the stable DNA phosphate backbone in normal gastric mucosa change into unstable DNA phosphate backbone in intestinal metaplasia issue under affecting of pathogenic factors, and finally, DNA phosphate backbone is broken and re-forms a double stabilized phosphate backbones, which causes the occurring of adenocarcinoma.

这些结果提示胃癌的发生过程中DNA结构变化过程可能是：胃粘膜正常组织具有稳定的DNA磷酸骨架，在致病因素作用下发展为具有不稳定磷酸骨架DNA的肠化组织，最后组织DNA磷酸骨架断裂并重新形成稳定的DNA磷酸骨架，致胃癌发生。

The junction sequences between T-DNA borders and the rice genome were determined for the left borders of 233 T-DNA insertions and the right borders of 190 T-DNA insertions. The results showed that truncations of T-DNA borders, insertions of filler DNA and the formation of inverted and direct repeat occurred during T-DNA integration.

分析了233个T－DNA左边界和190个'l&－DNA ／ti 边界与水稻基因组交接位点的序列，结果表明T－DNA在整合过程中不仅伴随着左右边界序列的缺失及&filler DNA的插入，而且还以单拷贝或多拷贝的形式插入在基因组中的同一个位点。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。