查询词典 DNA

In our study,two important lepidopteron pests,beet armyworm and cotton bollworm were surveyed for their endogenous piggyBac.As a result,endogenous piggyBac elements were found in these two insects,and one of the piggyBac elements isolated from the genome of cotton bollworm was intact and thought to be potentially active.1 Clonging and sequence analysis of piggyBac from beet armywormUsing PCR technique,with degenerate primers,a DNA fragment of piggyBac-like element was cloned from the genome of beet armyworm,spodoptera exigua hubner.The DNA fragment was 456bp in length,and the deduced amino acid sequence shares 50%- 78% similarity with other piggyBac elements from insects.But one stop code was found in this DNA fragment.

由于转座子很难用常规的RT-PCR和RACE技术进行克隆鉴定，故本研究在兼并引物PCR的基础上，利用inverse PCR和vectorret PCR技术，不仅调查了鳞翅目两种重要的农业害虫甜菜夜蛾和棉铃虫的內源piggyBac存在情况，同时克隆到了內源性的piggyBac转座子，并且从棉铃虫中获得了一个结构完整、具有潜在活性的piggyBac转座子HaPLE1.1甜菜夜蛾piggyBac转座子基因的克隆与序列分析采用PCR技术，利用兼并引物，从甜菜夜蛾基因组中克隆出一个內源性piggyBac类似因子的DNA片段，并命名为SePLE。

The ends of restricted DNA molecules can be joined together by ligation to create new recombinant DNA molecules .

DNA分子的限制性末端可以通过连接产生新的重组DNA分子。

Results Fifty-eight chronic liverish patients and 13 ASC persons were detected, in which HBV DNA levels with A83 mutations (log10) were (5.37±0.60),(4.02±0.51) copiesml^(-1) respectively. Ten blood donors were not found HBV A83 mutations.

结果 有HBV DNA前C区A83位点突变的58例慢性肝病患者血清中病毒变异株DNA水平为(5.37±0.60)copiesml^(-1)，13例无症状病毒携带者血清中病毒变异株DNA水平为(4.02±0.51)copiesml^(-1)，两者比较，t=7.54，P.001，差异有高度显著性；10例献血员血清中未检测到A83病毒变异株。

Analysis of the obtained genome amplification showed that DNA after PCR presented obvious polymorphism, and the results were the same using the same primer to amplify the larva and imago of the same species. It indicates that RAPD technique can be applied to the identification of dry samples and dipped larva sample of longicorn beetles as well as the research of phylogeny. These three methods for extracting genome DNA from different species of longicorn beetles at different preserved time are feasible.

对所获得的基因组扩增分析表明，经过PCR之后的DNA能呈现明显的多态性，同种天牛用同一引物扩增幼虫和成虫所获得的结果相同，可以认为，RAPD技术可以应用于天牛成虫干标本及幼虫浸渍标本的鉴定及系统发育研究中，也证明了这3种方法用于提取不同保存时间、不同种天牛基因组的DNA是切实可行的。

In these fragments, both the resting and the vegetative cell macronucleus DNA had 18 and 44 specific fragments, respectively.

在所选用的32条随机引物中，线粒体DNA共扩增出154条片段，其中以休眠细胞线粒体DNA为模板扩增出19条特有片段，以营养细胞线粒体DNA为模板扩增出25条特有片段，两者有29％的差异。

DNA average ploidy value and DNA ploidy type can be used as an important reference index for differential diagnosis and malignance of prostate cancer.

DNA平均倍体值、DNA倍体类型可作为前列腺癌鉴别诊断及判定前列腺癌恶性程度的重要参考指标。

The following five reviews published in this volume summarize recent discoveries in this area: DNA Replication and Transcription in Mammalian Mitochondria, Mitochondrial-Nuclear Communications, Translocation of Proteins into Mitochondria, The Machines that Divide and Fuse Mitochondria, and Why Do We Still Have a Maternally Inherited Mitochondrial DNA?

这一卷发表的以下五篇综述总结了这个领域的最新发现：哺乳动物线粒体中的DNA复制和转录，线粒体-细胞核通信，蛋白质到线粒体的迁移，分离和融合线粒体的机制及为什么我们仍然有一个母系遗传的线粒体DNA？

The DNA expansion instrument is an instrument for analyzing genetic factor-DNA molecular chains,which are widely used in biotechnology,medical jurisprudence,anthropology,archaeology and environment protection.

DNA扩展仪是用于对遗传基因——DNA分子链进行分析的仪器，用于生物技术、法医学、人类学、考古学和环境保护等广泛领域。

The technique of RAPD was used in analysis of mele or female also. Eachprimer among amplified bands of DNA is of polymorphic at various levels, and63％ of DNA fragments amplified are of polymorphic.

应用RAPD技术分析绞股蓝雌雄株，在DNA的扩增位点中，每一个引物均显示出不同程度的多态性，扩增出DNA片段总共有63％个位点是多态的。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。