查询词典 DNA

DNA C-value is of practical value in the forecast of the invasiveness of exotic species. However, such predictions must be made with comparisons within families because the DNA C-values vary greatly between woody and herbaceous species, between monocots and dicots, between annuals and perennials, and especially among families.

DNA C-值在预测外来物种的入侵性方面具有一定的应用价值，但是，由于在木本植物和草本植物之间、单子叶与双子叶植物之间、一年生和多年生植物之间，特别是在不同的科之间，植物的DNA C-值较明显的差异，因此，根据DNA C-值预测外来物种的入侵性，应该严格地限于同一科内的相关物种间的比较。

In order to calculate Melting Temperature in a more reasonable way, we use the SOFM of kohonen neural-networks to extract wild field features from DNA sequence, and a back propagation network is employed to predict Tm of DNA duplexes with these features.

本文以DNA序列的邻近法参数为基础，根据DNA序列热稳定性特点提出了DNA序列的编码方案，并建立了基于特征自组织网络的解链温度的预测模型。

DNA synthesis and unscheduled DNA synthesis was assessed by measuring the incorporation of 3H-TdR into DNA along with changes in hypocotyls protein content.

UV- B胁迫对 DNA的损伤还会影响基因的表达，改变细胞蛋白质组成和含量 [7，8] ，这与 DNA受

In both cases, when they grew the cells in the presence of a chemical that infiltrates newly synthesised DNA, they could see that the unlabelled template strands of DNA went into one daughter cell and the new strands went to the other.

他们在培养基中添加能渗入新合成DNA的化学试剂，结果，两种情况下都观察到未标记的模板DNA链进入了一个子细胞，而新合成的DNA链进入了另一个子细胞。

The binding molecular mechanism of R,R-SA-Pt and DNA was investigated by 13CNMR and DNA viscosity titrations.

结果：发现环方铂是通过方酸根的离去与DNA碱基之间形成共价键而结合的，而环斑铂是通过嵌插到DNA碱基对之间以非共价方式与DNA结合的。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Among them multi-types of body colour and special types of hosts are the most universal phenomena. In our experiment, methods of microsatellite primer PCR molecular marker and partial gene sequence analysis of mitochondrial DNA COⅠ-Ⅱwere used to analyze genetic diversity of two body colour bio-types (red bio-type and green bio-type) of green peach aphid from different plants and relationship between green peach aphid and host plants by separately selecting individuals of aphid from rape, Chinese cabbage and tobacco. The main results as follows: 1. Selecting An Ruisheng'method of extracting DNA from small sized insect, using this method can extract DNA well and steadily.

本文采用微卫星引物PCR 分子标记和线粒体COⅠ-Ⅱ部分序列分析的方法，分别选取十字花科植物中的甘蓝和油菜，茄科植物中的烟草，对不同寄主上桃蚜的红色和绿色两种体色生物型的遗传多态性及其与寄主植物的关系进行了研究，取得的主要结果如下： 1、选取安瑞生(2002)小型昆虫DNA 提取方法，能很好且比较稳定的提取桃蚜基因组DNA。

During blood cell deelopment, the remoal of DNA adducts, the resealing of repair gaps, the resistance to DNA-reactie drugs clearly increased in stem or mature compared with progenitor cells of the same indiidual.

在血细胞发育过程中，造血干细胞和相应的成熟细胞对致DNA突变剂的去除，DNA缺口的填补，DNA反应药物的抵抗明显高于对应得造血祖细胞。

We successfully identified JSRV mRNA expression and proviruses DNA in sheep lung tissues infected with JSRV and control group has no postive signals,Validating the use of exogenous virus-specific DNA probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences.

用地高辛随机引物法标记exJSRV特异的env片段，制备探针，原位杂交检测SPA肺组织中的RNA及前病毒DNA，结果表明SPA患羊肺组织内有JSRVenv基因mRNA的表达，同时也检测到了前病毒DNA，而相应的阴性对照却无阳性信号，证实外源性病毒特异的DNA探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。

An improved method that facilitates the extraction of PCR-compatible faecal DNA from giant panda\'s faeces is described. The method involves a novel preprocessing step in DNA extraction. The faeces is washed two or three times with precooled acetone, which removes numerous potential PCR inhibitors, and then digested with proteinase K. The DNA is purified with phenol/chloroform.

在粪便DNA的提取过程中采用一个新的预处理方法，将粪便用预冷的丙酮洗2～3次，除去粪便中含有的大量PCR抑制物，然后蛋白酶K裂解、酚-氯仿抽提，能提取到纯度很高的DNA供PCR扩增。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。