查询词典 DNA

Using the technologies of DNA synthesis, DNA clone, PCR and DNA chip and the theory of computational complexity, an encryption scheme is formed. In the scheme, encryption is to confect a specially designed DNA mixture and decryption is the hybridization of DNA sequances on a DNA chip.

利用DNA合成技术、DNA克隆技术、PCR扩增技术以及DNA芯片技术，结合密码学的计算复杂度理论，提出了一种基于DNA技术的加密方法。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

The central issue of this paper is the study of DNA-binding proteins interacted with DNA replication origin. The paper presented here includes there parts which are related as well as independent of each other:(1). Identification and partially purification of DNA-binding proteins interacted with eukaryotic DNA replication origin;(2) Specific binding of Hela cell proteins to Samian Virus 40 replication origin with the cruciform structure (Stem-Loop structure);(3) The study of DNA-binding protein as a tumor marker.

本文对DNA结合蛋白的研究主要包括三个即有关联，又相对独立的方面：（1）猿猴肾脏细胞复制起始区DNA结合蛋白的鉴定和部分纯化；（2）Hela细胞中DNA结合蛋白对含茎环结构的SV40复制起始区DNA的特异性结合；（3）DNA结合蛋白做为肿瘤征兆物的研究。

As metal ions can interact with both of ADM and DNA, they undoubtedly affect the interaction between ADM and DNA. Addition of a large excess of Cu or Fe into a solution of well-formed ADM-DNA complex makes the metal ions simultaneously bind to ADM and DNA to form a ternary complex. Otherwise,〓 or 〓 complexes can bind directly to DNA to form a ternary complex.

金属离子-ADM-DNA三元体系的光谱研究表明，当ADM与DNA结合后，大量Cu或Fe的加入可以使金属离子进入到ADM-DNA复合物中，并同时与ADM和DNA键合，形成三元复合物；反之，若ADM先与Cu或Fe配位，生成的金属配合物则能够直接与DNA结合形成三元复合物。

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇／卵磷脂／十八胺脂质体包裹，免疫2周龄SPF鸡，4周后用同型禽流感病毒进行人工感染，1周后采集泄殖腔分泌物分离病毒，结果未免疫组6／6分离到病毒，裸质粒DNA免疫组1／6分离到病毒胆固醇／卵磷脂／十八胺脂质体包裹DNA免疫组1／6分离到病毒，DC-chol脂质体DNA免疫组没有分离到病毒（0／6）：人工感染后1周各组的HI抗体（Log2）分别为6.38±0.98，7.00±1.26，7.83±1.17，8.00±0.89，2周后为8.50±0.55，8.67±0.82，8.68±0.45，9.33±0.52，脂质体包裹组在同期均高于未免疫组和裸DNA免疫组，表明脂质体包裹质粒DNA免疫动物后，能增加动物对病毒感染的抵抗力和反应能力。

Using large sample DNA of capacity technology, the maize high lysine content of SSR mark analyse technological platform has been established in this paper. The main results are as follows: the five comparing research methods of drawing maize DNA mark have shown that the method of iso- sulphur cyanic acid guanidine drawing maize genome DNA has the superiority of using less medicine, operating sequence more simple , faster, and costs lower , steady and reliable quality of DNA, etc..Utilizing this method , one person can draw 300-400 DNA samples each workday.lt indicates that the method can be used in SSR marker of maize,.

本文从大样本容量的DNA快速提取技术入手，初步建立了玉米高赖氨酸含量的SSR分子标记分析技术平台，获得的主要结果如下：对五种玉米DNA提取方法的比较研究认为，异硫氰酸胍法提取玉米基因组DNA，具有使用药品少、操作步骤简单、快速、成本低、DNA质量稳定可靠等优点，利用该方法，每人每个工作日可以提取300～400个DNA样品，可以在玉米SSR标记分析中应用。

Although the biological significance of DNA methylation has been recognized to some extent, the mechanisms of DNA methylation regulation and of the establishment of tissue-specific DNA methylation pattern in somatic cells are still unclear, since the knowledge of the enzymes responsible for de novo DNA methylation and demethylation of DNA is very limited and up to now only two functional DNA methyltransferases in mammals have been cloned.

目前，虽然对DNA甲基化的作用已有一定认识，但DNA甲基化的调节以及发育过程中组织特异的DNA甲基化谱的建立机制还不清楚。问题主要在于对催化重新甲基化和去甲基化反应的酶的了解很少。至今仅克隆出两种有功能的哺乳动物DNA甲基转移酶。

As well using flameless atomic absorption spectophotometer toanalyse the amount of platinum binding to DNA,we can fand that after 1hour at 37℃ the amount of platinum binding to DNA for BGC-823 acted byCDDP 20ug/ml in hypotonic solution was 30.1±6.2ng/mg·DNA,whilethe isotonic groups were 6.4±1.5ng/mg·DNA.

采用非火焰原子吸收光谱法检测DNA结合铂含量发现，BGC-823经20μg／ml CDDP，37℃作用1小时，低渗给药组DNA结合铂含量为30.1±6.2ng／mg·DNA，等渗给药组DNA结合铂含量为6.4±1.5ng／mg·DNA，两者差异显著(P＜0.05)，该结果表明低渗溶液能显著增强CDDP与其作用靶点—DNA的交联。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

The experimental results show that: DNA of normal gastric mucosa has stable phosphate backbone; Since the peak intensity at 1090 cm^(-1) is lower than that at 1050 cm^(-1), phosphate backbone in DNA of intestinal metaplasia becomes unstable, while the rest spectra peaks are similar to those in normal gastric mucosa; In terms of adenocarcinoma DNA, double peaks at 1090 cm^(-1) whose intensity are higher than 1050 cm^(-1), are observed, which indicates that DNA chain is broken and re-forms a double stabilized phosphate backbones. In addition, peaks at 950 cm^(-1), 1010 cm^(-1), 1100~1600 cm^(-1) also exhibit obvious difference compared with normal gastric mucosa, which shows that deoxyribose and bases are changed due to broken of DNA.

实验结果表明，胃粘膜正常组织DNA中有稳定的磷酸骨架；肠化组织基因组DNA拉曼特征峰在1090 cm^(-1)处谱峰强度低于1050 cm^(-1)处谱峰强度，表明其磷酸骨架变得不稳定，其它位置的谱峰与正常组织相似；腺癌组织基因组DNA拉曼特征峰在1090 cm^(-1)附近出现双峰，其相对于1050 cm^(-1)处谱峰强度更强，提示DNA有可能出现断链并再次形成了稳定的磷酸骨架，在950 cm^(-1)、1010 cm^(-1)、1100~1600 cm^(-1)波段的特征谱峰与正常组织DNA相比变化也较大，说明脱氧核糖和碱基可能由于DNA断链也随之改变。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。