查询词典 DNA

However, the museum C. heterodon specimens were preserved in formalin. It is necessary to approach the methods of effectively extracting the genomic DNA from formalin-fixed C. heterodon, and the feasibility of using the extracted DNA in microsatellite-and mitochondrial analyses. In the present paper, the genomic DNA of ten formalin-fixed C.

然而，过去的铜鱼标本都保存在福尔马林溶液中，有必要探讨从福尔马林固定的铜鱼标本中有效提取基因组DNA的方法以及这些DNA用于微卫星和线粒体分析的可行性。

The results indicate that DNA can obviously increase the fluorescence intensity ofγ-MAG, the part intercalative binding is the major model forγ-MAG and DNA, the ionic strength has an obvious effect on the interaction betweenγ-MAG and DNA.

结果表明，DNA的存在使得γ-MAG的荧光强度增强，DNA以部分插入方式与γ-MAG发生作用，且离子强度对二者的作用有比较明显的影响。

Displayed similar photophysical changes in the presence of DNA. But the complex increased the viscosity of DNA weakly. This suggests that due to the constraint from the rigid structure of the macrocyclic ligand, the complex binds to DNA by weak intercalative interaction.

在光谱方面也出现了类似的现象，但DNA的粘度却略有增加，说明环上的苯环可能由于受到整个配合物结构刚性的限制，只能以较弱的插入方式与DNA作用。

The improved CTAB method was applied to extracted genomic DNA in the leaves of 7 species of Michelia. Using the method of single factor in the experiment, the effects of different concentration of MgCl2, dNTP, template DNA, random primers and Taq DNA polymerase on RAPD reaction were studied.

方法］采用改进的CTAB法提取木兰科含笑属7种植物叶总DNA，进行RAPD分析，分别研究了模板DNA、引物、dNTP、Mg(上标 2+)和TaqDNA聚合酶用量对RAPD反应的影响。

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

近年来脑胶质瘤的基因治疗发展迅速，应运而生的方法有自杀基因、免疫基因、多药耐药基因以及抗血管生成基因等，其中自杀基因被认为是最有前景的基因治疗方法，它又称病毒介导的酶／药物前体疗法，是利用转基因技术将哺乳动物细胞中所不含有的自杀基因转入到哺乳动物肿瘤细胞中，该基因表达的产物可将无毒的药物前体转化为毒性药物，从而选择性杀伤该肿瘤细胞，常用的自杀基因有单纯疱疹病毒-胸苷激酶基因和大肠杆菌胞嘧啶脱氨酶基因，前者催化无毒性抗病毒核苷类似物如丙氧鸟苷、无环鸟苷等成为单磷酸核苷衍生物，然后在内源性细胞激酶作用下转化为具有明显毒性的三磷酸核苷，作为DNA合成链的终止剂和DNA合成酶的抑制剂，干扰细胞DNA的合成；后者编码的胞嘧啶脱氨酶可催化5-氟胞嘧啶（5-FC）脱氨成为5-氟尿嘧啶（5-FU），然后代谢为有毒性的5-氟尿嘧啶-5′三磷酸（5-FUTP）和5-氟-2′脱氧尿嘧啶-5′磷酸（5-FdUTP），5-FUTP通过与UTP竞争性结合而抑制mRNA和tRNA的合成，5-FdUTP则作用于胸苷合成酶，导致TMP衰竭而阻止DNA的合成，最终诱导肿瘤细胞凋亡。

Although DNA of high concentration might give rise to the quenching of IgG fluoresence, DNA of low concentration had but little effat on the nounal IgG fluormence.

DNA时IgG空间结构影响很小，虽然高浓度的DNA有熄灭IgG分子萤光的作用，但是低浓度DNA对正常人血清IgG的萤光几乎没有影响。

To determine whether F3, similar to other large DNA iruses, encoded proteins that lock or delay apoptosis, mock- and F3-infected FHM cells were osmotically shocked and assayed for DNA fragmentation 3 hours later. NA fragmentation was clearly seen whether or not shocked cells were preiously infected with F3, indicating that infection with F3 did not block apoptosis induced by osmotic shock in FHM cells.

为了证明F3是否象其大DNA病毒一样编码能阻止或延迟凋亡的蛋白，在模拟感染和正常感染的FHM细胞被渗透压休克后3小时检测DNA片段化；结果显示无论是否休克F3感染都能诱导FHM细胞的DNA片段化，渗透压休克并不能阻止细胞凋亡。

Correlation coefficient: 0.9962 . Positively charged'free'complex ions should be readily quenched by 〓, but when it is bound to DNA, it can be protected from the quencher because the highly negatively charged 〓 would be repelled by the negative DNA phosphated backbone, hindering quenching of the emission of the bound complex.

从理论上讲，带正电的配合物的阳离子应该可以被大阴离子淬灭，但是在DNA存在下，金属配合物插入到DNA的碱基对中，而〓被带负电荷的DNA磷酸骨架强烈排斥，从而避免了〓的淬灭，因此大的Stern-Volmer曲线斜率相应于对配合物较弱的保护形式。D。

The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

The experiments confirm that Nuclear fast red bindto DNA to form a 1:1 association complex with the binding constant 7.29×10~5 L/mol,and the major binding mode of Nuclear fast red to DNA is electrostatic binding.However, at high iron strength, the major binding mode changes into intercalativebinding. Part four is about investigation on the interaction of purpurine with DNA.

结果显示，核固红与DNA主要以静电作用模式结合，结合位点为1，结合常数为7.29×10~5L／mol；而在高离子强度下，其主要作用模式改变为嵌插作用；第四部研究了红紫素与DNA的相互作用。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。