查询词典 DNA

This paper reviewed the advances in honeybee breeding involving to estimate the numbers of subfamilies in a colony and analyze the genetic correlation among workers; guess the polyandry level of queens and identify the purity of a subspecies; build a linkage map and discriminate loci of quantitative traits; genotype and distinguish full sister from semi sisters; analyze the trend of sperm to inseminate an egg and so on by means of such molecular biotech as microsatellites DNA, mitochondria...

综述了应用微卫星 DNA技术、线粒体 DNA技术、随机扩增多态性 DNA、聚合酶链式反应和 DNA指纹等分子生物技术所进行的蜜蜂育种研究现状。其应用主要包括估计蜂群内亚家系的组成数目和分析工蜂间的遗传相关，推断蜂王的多雄水平和鉴定蜜蜂种性，构建连锁图和区别数量性状位点，鉴定基因型和区别全胞、半胞姐妹以及分析精子受精力的倾向性等

In order to further study, with a new method of preparation of DNA fibers which has conquered the disadvantage of the way of squashing in liquefacient nitrogen,high-quality fibers were been gotten from maize which has large genome and contains numerous types of highly repeat DNA elements.

为了进一步分析，对现有的提核方法进行了改进，采用了新的方法制备DNA纤维，克服了液氮研磨法的不足，并在基因组比较大，重复序列较多的玉米上获得成功，同时用伸展DNA纤维的荧光原位杂交技术测定了端粒DNA在玉米染色体上的拷贝数。

DNA ANALYSIS OF BLOOD AND TISSUE SAMPLES TAKEN FROM INVID SIMULAGENT (A.K.A "MARLENE,""ARIEL") PROVE UNEQUIVOCAL MATCH TO ON-FILE DNA PROFILES OF MARS DIVISION E.R. TECH MARLENE RUSH.

从 因维模拟人（也叫做"玛琳""艾丽尔"）的血液和组织样品的DNA分析表明，她的DNA组成与有记录的火星师技师玛琳·拉什的DNA档案完全相同。

By employing two kinds of molecular marker of mitochondrial DNA, i. e. Cyt b and CoI gene, the sequences of 15 species representing 6 families from Passeriformes were compared. We analysed their sequences compose and characteristic with the sorft of MEG3.1, and built the phylogenetic trees in PAUP~*4.0. We deeply analysed the feasibility that CoI gene qua the marker to research the phylogenetic relationship of Passeriformes, and afford the academic proof for building the DNA barcoding of the birds.

本文分别以线粒体DNA的细胞色素b和CoI基因作为分子标记，对雀形目6科15种鸟类的细胞色素b基因全序列(1，143bp)和CoI基因部分序列(1，176bp)的组成特点及其系统发育树进行了比较分析，还探讨了CoI基因作为雀形目系统发育研究和构建鸟类物种DNA编码库的分子标记的可行性，旨在为DNA编码库的构建积累基础资料。

By directly imaging the Brownian motion of the polystyrene bead, which is tethered to the coverslip through a DNA molecule, TPM allows the real-time measurement of DNA length change during enzymatic processes.

利用高解析的光学显微技术，我们得以准确地观测接连在DNA及玻片表面的乳胶小球之布朗运动情形，并藉由其布朗运动的大小直接对应到在生化反应中DNA长度的变化，因此单分子拴球实验已被广泛地应用於研究单一蛋白质与单一DNA分子作用之反应机制。

The results showed that a highly significant increase in DNA migration in DNA-damaged lymphocytes was induced by three different concentrations of yttrium and praseodymium compared with the control,The percentage of DNA-damaged have genetic toxicity.

结果表明，与对照相比，3种不同浓度的氯化钇和氯化镨均可引起淋巴细胞DNA受损后DNA迁移率的显著升高，受损伤细胞的百分率与对照差异明显，提示氯化钇和氯化镨具有一定的遗传毒性。

The total DNA has been extracted from the fresh leaves of 4 medicinal species of pteridophyte with CTAB, SDS, to compare their effects on DNA extraction. The result shows that the optimum preparation method obtained to extract high-quality DNA lays theoretic and practical base for PCR amplification and primer design, for filtrating related gene of the active elements from leaves of the four pteridophytes.

以四种药用蕨类的叶片为材料，采用改进的CTAB法、SDS法分别对四种蕨类进行了总DNA提取效果的比较分析，结果表明，通过实验获得最佳的DNA提取方法，为PCR扩增，引物设计，为筛选四种药用蕨类中的活性成份的相关基因奠定理论和实践基础。

They include the variation of the degradement velocity of different sample, the inhibition product of PCR, the amount of DNA extraction, quality of DNA and the heteroplasmy of some tissue. Correct sequencing, comparison of the result of forward and reverse primers sequencing should be done to rule out wrong signals, and prevent contamination. In addition to gene sequencing, low grade heteroplasmy could be dealt by Denaturing high performance liquid chromatography. Multiplex Ligation-dependent Probe Amplification. The analysis of mitochondrial HV Ⅰ, HV Ⅱ sequencing is able to identify the maternal sanguinity, then it could assist in the human identification. It also complements the genomic STR system in distribution of population, the origin of human, and analysis for fossil.

分析粒线体DNA时必须考虑各类检体腐败DNA降解速度之不同、PCR的抑制物质、DNA的萃取量及品质、特定组织的异质现象、萤光序列分析图及正、反向引子定序结果比对、排除杂讯干扰、防治污染等，除以基因定序外，低程度的异质现象也可用Denaturing high performance liquid chromatography、Multiplex Ligation-dependent Probe Amplification等方法分析，粒线体HV Ⅰ、HV Ⅱ碱基序列的分析可鉴别一个人的母系血缘关系、辅助人类身分鉴定，对於族群分布、人类起源探讨、远古检体的分析可作为辅助STR系统的利器。

The results showed that RSAP used two primers of 18 nucleotides in which starting at the 3' end of each primer were restriction site sequences (4-6 bases),followed 12-14 bases of arbitary sequence, the differences between two primers were restriction sites and filler sequence; PCR am-plification was run for the first 5 cycles with an annealing temperature of 35℃, followed by 35 cycles with an annealing temperature of 48℃; the PCR reaction of 25 μL included 20ng DNA templates, 2.5mmol/L of Mg(superscript 2+), 0.2 mmol/L of dNTP, 1.5U of Taq DNA polymerase, 600nmol/L of each primers. RSAP is a new maker technique with simplicity, moderate throughput ratio and reliability. RSAP is of good reprodicibity and broad application.

结果显示，RSAP技术的引物为2条长度均为18bp的引物，引物的3'端为4~6个碱基的限制性酶切位点序列，接着是12~14个碱基的随机序列，2条引物的限制性位点和随机序列不同：PCR扩增的前5个循环采用35℃的退火温度，随后的35个循环采用48℃的退火温度；在25μL反应体系中，模板DNA用量为20ng，Mg(上标 2+)浓度为2.5mmol/L dNIPs浓度为0.2 mmol/L，Tap DNA聚合酶用碳为1.5U，2条引物浓度均为600mmol/L；RSAP技术重复性好，适用性广泛，是一种操作简便、产率中等、稳定可靠的DNA标记技术。

The results obtained by electron microscopic observation show that the supercoiled pUC18 DNA adopts a doughnut-like solenoidal structure with outside diameter about 43.8 nm and inside diameter about 2.0 nm in ammonium acetate-H2O medium.

观察结果显示：在疏水性的甲酰胺-水展开系统中，DNA采取通常的互缠式结构；在含有1.5 mmol/L醋酸铵的水介质中制备的超螺旋DNA标本，DNA采取线圈型结构，测得pUC18 DNA分子这种结构的外直径约为43.8 nm，内直径约为2 nm。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。