查询词典 DNA

The changes of amyloplast and amyloplast DNA in different development stages were also observed, indicating the relationship among the amyloplast core, crystal structure and the amyloplast DNA.

近十余年来对淀粉体DNA的研究也有些工作〔3一"'，我们在几种植物贮藏组织淀粉体DNA的研究中〔"川，观察到淀粉体DNA在不同的材料中具有分布形态的多样性，淀粉

Mitochondria in mammalian cells harbor their own DNA, but unlike nuclear DNA, the mitochondrial genome is transmitted asexually from mother to child,and thus does not benefit from genetic recombination.

哺乳动物细胞中的线粒体包含有它们自身的DNA，但与核DNA不同，线粒体DNA是通过无形繁殖从亲代遗传给子代的，因此遗传重组对mtDNA无益。

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

Consequences of the rotation of DNA are illustrated in Figure 9.17 in the twin domain model for transcription. As RNA polymerase pushes forward along the double helix, it generates positive supercoils ahead and leaves negative supercoils behind. For each

超螺旋与转录还有更多的关系，RNA聚合酶转录DNA时，解链和复链同时也发生，如同Fig113中所示，这需要整个转录复合体绕DNA旋转或者DNA自己绕它的双链轴旋转，DNA旋转的后果如Fig11.19所示，图中是一个转录的双结构域模型，当RNA聚合酶沿DNA前移时，在酶前产生正双螺旋，酶后为负的双螺旋；每一个双螺旋转角被RNA跨越后都会在前面产生+1转角，后面产生-1转角。

In chapter 1, the composition of DNA, the basic mode in DNA binding complexes, current research interesting in this field, as well as major processes made in this thesis are briefly introduced and research methods concerning complex DNA-binding and investigation review about chemical nucleases are the emphases.

第一章前言简要介绍核酸的组成和结构、配合物与DNA相互作用的基本形式以及金属多吡啶配合物与DNA相互作用的研究概况；重点讨论了金属配合物与DNA作用的常用研究方法和有关化学核酸酶的研究进展。

Moreover, the protein-free spreading-free spreading technique, involvingbenzyldimethyl-alkyammonium bromide and the denaturing agent formamide, wasestablished. With very low DNA concentration, the fully extended DNA samples for linearDNA can be prepared.

采用Sephadex色谱柱分离，保温稀释的DNA溶液，不仅可除去DNA链上吸附的盐，而且可使盘绕一起的DNA链部分展开；在此基础上，通过采用苯二甲基辛基溴化胺－甲酰胺微量展层技术，可使纳克级DNA完全展开到云母基底上。

First,Tester DNA sample was prepared from tumorDNA and Driver DNA sample from normal DNA derived from the same patient ofgastric cancer.

以胃癌组织DNA作为Tester，癌旁组织DNA作为Driver，按1∶400和1∶40，000的比例进行两次消减杂交和PCR扩增得到了3条胃癌组织DNA中特异存在的基因片段1A360，1A210和1A160。

As a result,1A360 probe did hybridize strongly to Tester DNA sample,but weakly to Driver DNA.Thus,our two rounds of RDA are proved to be specific andeffective.Because of not hybridizing to the liver tissue DNA of rat and the thymicDNA of bull,so the probe is species-specific and gained from human chromosome.

该探针不能与鼠肝组织和小牛胸腺组织DNA杂交，说明它来源于人源染色体并有种属的特异性。1A360基因片段探针还与多种胃癌细胞系，胃癌组织DNA有较强的杂交信号，与其它肿瘤DNA有强弱不等的杂交信号。

Closed loop DNA does not have fracture double strand ring-like DNA, is also called ultra screw DNA.

闭环DNA没有断口的双链环状DNA，亦称为超螺旋DNA。

Effect of TP on DNA primase-polymerase α complex activity was examined using Liquid Scintillation counter. Results:It showed that IC50 value of TP inhibiting DNA primase-polymerase α complex activity was 20.28 (15.17～ 27.13)μ g/ml. TP could promote DNA primer synthesis but inhibit DNA primer extension.

结果：茶多酚对小鼠肿瘤L2细胞DNA引物酶多聚酶α复合体活性有抑制作用，半数抑制浓度（IC50）值为20.28(15.17～27.13)μg/ml；对DNA引物合成有促进作用；对引物延长亦有明显抑制作用，其IC50值为36.43μg/ml。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。