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Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰,直径约1mm,成斑时间12h;从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号: AY639599),又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因,表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli, E.coli)中表达获得了47kDa的清晰表达带,在1h 时开始产生蛋白并有逐步上升的趋势; Western blot 也在47kDa处得到一条清晰的条带;可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的,该蛋白的产生明显地抑制了宿主的生长速度;噬菌体PEP氨基酸序列之间的同源性比较表明,噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Chicken Meal, Chicken, Brown Rice, Duck Meal, Barley, Oatmeal, Chicken Fat (Preserved With Natural Mixed Tocopherols), Potatoes, Carrots, Lamb Meal, Salmon Meal, Canola Oil, Natural Flavor , Dried Egg, Brewers Yeast, Whole Ground Flaxseed, Potassium Chloride, Choline Chloride, Taurine, Spinach, Parsley Flakes, Cranberry, Lysine, L-Carnitine, Yucca, Kelp, Zinc Proteinate, Vitamin E Supplement, Niacin, Manganese Proteinate, Copper Proteinate, Zinc Sulfate, Manganese Sulfate, Copper Sulfate, Thiamine Mononitrate (Vitamin B-1), Vitamin A Supplement, Biotin, Potassium Iodide, d-Calcium Pantothenate, Riboflavin (Vitamin B-2), Pyridoxine Hydrochloride (Vitamin B-6), Vitamin B-12 Supplement, Manganous Oxide, Sodium Selenite, Vitamin D Supplement, Folic Acid.

鸡肉粉;鸡肉,糙米,鸭肉,大麦,燕麦,鸡肉脂肪,马铃薯、胡萝卜,羊肉粉,鲑鱼粉,芥菜油,天然香料,蛋粉,啤酒酵母;卵磷脂;亚麻仔,氯化钾,氯化胆碱,菊糖;牛磺酸,菠菜,荷兰芹,蔓越莓,赖胺酸,左旋肉碱,丝兰,海带,锌蛋白,维生素E补充,菸碱酸,锰蛋白,铜蛋白,硫酸锌,硫酸锰,硫酸铜,硝酸硫胺明(维生素B1来源),维他命A,维生素H,碘化钾,D-泛酸钙,维生素B-12 补充;核黄素(维生素B-2来源),维生素D补充,叶酸。

Methods: By prospective study, the ovarian artery blood flow, which included S, D, PI, RI, and S/D, was detected by transvaginal color Doppler in 164 COH cycles both on second day of menstruous cycle (M2) and the day of HCG administration.

应用前瞻性研究法,随机选择164个超排卵周期,经阴道彩色多普勒B型超声,监测月经第2天(M2)和HCG日卵巢动脉血流S,D,PI、RI和S/D。

The main gluconeogenic precursor in kidney is thought to be lactate; however, less is emphasized enantiomerically. L-lactate is a glycolysis end-product, but D-lactate is formed after detoxification of methylglyoxal, which is the main source of advanced glycation end-products.

乳酸为肾脏糖质新生的主要来源,其含有一不对称碳,故具有D-、L-乳酸两种镜相异构物,而D-、L-乳酸两者之生成相当不同,L-乳酸是糖解作用之终产物,D-乳酸为体内一醣化终产物(advanced glycation end-products)─甲基乙二醛进行去毒化反应所生成,目前缺乏对乳酸镜像异构物与肾脏糖质新生间相关的探讨。

They all contain 13 protein coding gene,2 rRNA gene,22 tRNA gene and 1 D-Loop.The base composition for the four nucleotides is A-32.0%,C-27.6%,G-14.7%,T-25.8%,And it is A-32.5%,C-26.9%,G-14.1%,T-26.5%for Yellow-throated Marten.But there are some definite differences in base composition,the using of Initiation codon and Stop codon,and the mode of repeat sequences in control region.The codon usages of Manes have bias,and the ttiird locations of codon of protein-coding genes have the higher frequency in using A and C.There may be some relativity with the content of A and C in D-loop,namely,it has relation with the mode of repeat sequences.The complete mitochondrial genome of the Sable and Yellow-throated Marten were submitted to GenBank,and the accession number are FJ429093 and FJ719367 respectively.3、The complete mitochondrial genome of 6 other species of Mustelidae from GenBank and some sequences of D-loop from the 6 species were aligned.

分析紫貂大兴安岭亚种、长白山亚种、阿尔泰亚种和北欧亚种间的基因流及进化历史得知:大兴安岭种群与新疆种群和长白山种群间的基因流水平最高(Nm=0.1260和0.1427),新疆与长白山种群间最低Nm=0.0053紫貂种群在进化过程中可能发生过种群膨胀,经历过种群增长过程。2、对紫貂和黄喉貂的线粒体全基因组结构进行分析发现:全长分别为16 523bp和16549bp,均包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码序列区(D-Loop区,紫貂全序列中碱基组成为A-32.0%,C-27.6%,G-14.7%,T-25.8%,黄喉貂为A-32.5%,C-26.9%,G-14.1%,T-26.5%;基因排列顺序与日本貂和貂熊的一致,但碱基组成、起始密码子和终止密码子的使用及控制区中串联重复序列模式等均存在一定差异。

Simulate the condition of temperature and energy control in percutanous MWA for HCC to ablate SMMC-7721 cell strain suspension.There were five experiment groups:A.control group,B.45℃10 min group,C.50℃3min group,D.54℃3min group and E.60℃instant group.Detection for apoptosis was performed at 30 minutes and 2 hours after ablation.2.Thirty-two C57 mice were undertaken hypodermic inoculation of Hepal-6 tumor piece at right groin as ablation tumor and at left groin as observation tumor one week later.The mice with double tumors were separated into four groups:A.control group with no treatment,B.MWA group treated with MWA only,C.mDC group treated with MWA and subcutaneous injection of mature dendritic cells sensitized by neoantigen of Hepal-6 cell strain,D.iDC group treated with MWA and peritumoral injection of immature dendritic cells without neoantigen sensitization.

模拟肝癌微波治疗中常用消融区边缘的温控和能量条件,消融人肝细胞癌株SMMC-7721细胞悬液,分为五组:A、对照组,B、45℃10min组,C、50℃3min组,D、54℃3min组,E、60℃即刻组,消融后30分钟及2小时进行凋亡检测。2.32只C57小鼠左侧腹股沟皮下接种Hepa1-6鼠肝癌细胞瘤块作为消融瘤,间隔1周,右侧腹股沟皮下接种瘤块作为观察瘤,成瘤后分为四组:A组,对照组,不行治疗;B组,微波组,仅微波消融小鼠消融瘤;C组,成熟DC组,微波消融小鼠消融瘤,并于小鼠双侧腋下注射肿瘤抗原致敏的树突状细胞;D组,未成熟DC组,微波消融小鼠消融瘤,并于消融瘤周注射未成熟树突状细胞。

One-axial loads as well as proportional and nonproportional multiaxial loads such as torsion and bending with and without phase shift (d= 0 deg and d=90 deg) were applied to the welded conxtruction samples.

一轴向负载,以及成比例和nonproportional multiaxial象扭并且有和没有相移(d = 0 度和d = 90 度)弯曲那样装被用于被焊接的conxtruction 样品。

Effects on reproductive performance: The normal delivery of group B,C and D were 80%、93.33%、93.33%,respectively,were improved by a certain extent comparing with the control group; The mean birth weight of calves were not significantly different between the trial and control groups.But numerical datas shown that supplementing Zn-Met in dietary had the tendency to increase the birth weight of calves; Supplementing Zn-Met in dietary can make dairy cows express empathema earlier,and significantly(P.05).Among the trial groups,the advanced extent of the group C was comparatively large(5.8d);The oestrous cycles were all in normal range and not significantly different; The levels of serum PRL of group B,C and D were significantly(P.05) 1.46 ng/ml、3.08 ng/ml、3.45 ng/ml higher than the group A.

对繁殖性能的影响:添加蛋氨酸锌组的正常分娩率为80%、93.33%、93.33%,较对照组(73.33%)有一定程度的改善;试验组犊牛初生重与对照组犊牛初生重差异不显著(P>0.05),但数据结果显示,日粮添加蛋氨酸锌有使犊牛初生重增加的趋势;日粮添加蛋氨酸锌可使奶牛产后发情提前,且差异显著(P<0.05),其中,添加蛋氨酸锌240mg/kg组提前的幅度较大,提前了5.8d;试验组发情周期与对照组差异不显著(P>0.05),均在正常范围之内;B、C、D组血清促乳素含量均比对照组高,且差异显著(P<0.05),依次增加1.46ng/ml、3.08ng/ml、3.45ng/ml,试验组B与C、D两组间也达到显著差异(P<0.05)。

The structure analysis indicates that the reverse symmetry ligand H4LB molecules are linked into a 2D supermolecular network by two types of hydrogen bonds. One type occurs between the oxalamide oxygen group and the phenol hydroxyl qroup of the nearest molecule with d=2.906(2) and ∠O-H…O=151.8°. The other type is between the oxalamide nitrogen group and the oxalamide oxygen group of anther molecule with d=2.849(2),∠N-H…O=158.7°.

晶体结构研究表明配体H4L为顺式桥联分子,晶体中存在两种氢键,一种是草酰胺基团的O原子与苯环上的酚羟基形成O-H…O氢键[d=2.906(2),∠O-H…O=151.8°];另一种是草酰胺基团的氨基与相邻草酰胺基上的O原子形成N-H…O氢键[d=2.849(2),∠N-H…O=158.7°],通过上述两种氢键将化合物H4LB连接成二维网络结构。

Professor Wang considered that D-IBS had the intimate relationship with liver,spleen and kidney and regard that weakness of the spleen and stomach is the foundation of pathopoiesis,hepatosplenic disorder is the key point of pathogenesis,asthenia of both the spleen and kidney is the important pathogenesis evolution.

腹泻型肠易激综合征发病和病机的理论研究:论文首先总结了祖国医学对腹泻型肠易激综合征的认识,包括对症状、病名及病位描述、病因病机及证治沿革和当代中医对D-IBS病因和发病机制的认识;继之对西医学对D-IBS病因和发病机制的认识做一简述,最后将导师对D-IBS发病和病机的认识和创新进行阐述。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。