查询词典 Colorimetry

Methods According to Chinese standard analysis method of foods (GB/T 5009133-2003), 108 samples of cured meat products were determined nitrite content by naphthyl ethylenediamine hydrochloride colorimetry. The results were analyzed.

采用国家食品卫生检验标准方法(GB/T 5009133-2003)即食品中亚硝酸盐与硝酸盐的盐酸蔡乙二胺比色法对市场上抽检108份腌腊制品中亚硝酸盐含量进行测定，对测定结果进行分析。

Methods: Rat MSCs were isolated with percoll and nonadherent cells were discarded 48 hours later. The shape was observed and CD34, CD44, CD45, CD166 were detected with Flow cytometry analysis. The growth curve of MSCs in different concentration of fetal bovine serum was observed by MTT colorimetry. The growth curve and attachment efficiency of MSCs of passage 1 (P1), P3, P5 and P10 were also observed.

采用密度梯度离心法结合贴壁分离法分离培养大鼠骨髓MSCs，观察其形态，流式细胞仪检测细胞表面标记，MTT法观察不同浓度的胎牛血清对MSCs生长曲线的影响及不同代MSCs的生长曲线和细胞贴壁率。

Methods MTT colorimetry,a silver colloid technique to identify nucleolar organizer regions and immunohistochemical methods were used to study the effects of the two inhibitors on growth of fibroblasts.

用MTT比色、AgNORs银染和免疫组化技术，观察两种抑制剂对成纤维细胞增殖、AgNORs合成和Ⅲ型胶原表达的影响。

Methods:Culture of aorta SMC in vitro,abdominal injection medication and drawing blood through ophthalmectomy,cytotoxic crystal violet staining for measurement of TNF, and dependent-cells MTT colorimetry for measurement of IL-6 were all performed.

体外主动脉SMC培养，腹腔注射给药及眼球摘除取血，细胞毒结晶紫染色法测定TNF，依赖细胞株MTT比色法测定IL-6。

The hydroxyl free radical scavenging rate was determined by phenanthroline spectrophotometry, and the antilipoperoxidant was determined by colorimetry.

采用邻二氮菲分光光度法测定经自由基的清除率，比色法测定其对脂质过氧化的抑制作用。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

According to the essays, we can use colorimetry, GC, TLC-scanning and HPLC for the determination of pseudoephedrine hydrochloride.

根据文献报道，盐酸麻黄碱的含量测定方法有比色法、气相色谱法、薄层扫描法和高效液相色谱法。

The trace elements and flavonoids were analyzed by ICP-AES and AlCl3 colorimetry, Results show that the content ranged from 48.0-526.8μg/kg and there were abundant zinc in Hibiscus sabdariffa and Tribute Chrysanthemum.

实验测得微量元素中有益微量元素锌含量范围为48.0-526.8μg/kg，玫瑰茄和贡菊含有丰富的锌；茶花、玫瑰茄含有丰富的锰等；而毒性微量元素铅的含量范围是1077.1～16157.8μg/kg，都未超标。

The immundogical function and cytokine levels in the serum of the tunor-bearing mice were determined by MTT colorimetry,hemolysis spectrophotography,improved Mayer's method and ELISA.

分别采用溶血分光光度法、改良的Mayer法及双抗体夹心ELISA法，检测荷瘤小鼠的免疫功能及细胞因子含量变化。

Oils ; colour ; Lovbind colorimetry ; F Lovbind tintometer ; PFX995 automatic Lovbind tintometer ; comparison

油脂；色泽；罗维朋比色法； F型罗维朋比色计； PFX995型罗维朋自动比色计；比较

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。