查询词典 Chain Reaction

Bacteria genomic DNA was extracted to do polymerase chain reaction to amplify specific DNA and then to assess coden sequence.

萃取细菌DNA后利用聚合酶连锁反应将细菌特定的DNA片段增幅，定序比对标准序列判定基因型。

Methods The samples were collected from 55 consecutive patients with tissue diagnosed lung cancers by fiberoptic bronchoscopic brushing. Mutation in coden 12 of H-ras oncogene was detected in the exfoliated cells using PCR-RFLP method (polymerase chain reaction-restriction fragment length polymorphisms).

对33例吸烟肺癌和22例不吸烟肺癌患者采用纤维支气管镜毛刷刷取支气管脱落细胞，应用PCR-RFLP方法进行H-ras基因突变检测。

The cDNA squence of antibiotic, an antibacterial peptide, a process for preparing the said cDNA and a process for recombining the antibacterial peptide are disclosed. The present frequency of amino acid codens in Attacin A cDNA is analyzed. The high-frequency coden is introdued to its protein sequence to configure its cDNA sequence. The target gene is synthesized by chemical synthesis method or polymerase chain reaction.

本发明公开了葛佬素的cDNA序列，葛佬素cDNA的制备方法以及重组抗菌肽葛佬素的制备方法；通过对同种已知蛋白质Attacin A cDNA中氨基酸密码子的出现频度进行分析，采用出现频度高的密码子带入葛佬素蛋白质序列中进行葛佬素的cDNA序列构建，用化学合成法及聚合酶链式反应进行目的基因的合成，另外，采用基因重组法制备抗菌肽葛佬素，并测定了其活性。

The point mutations of the 12th coden of ras gene and p53 E5 and E7 gene were studied in 40 cases w ith laryngeal squamous cell carcinomas by PCR-SSCP(polymerase chain reaction-s ingle stranded conformational polymorphism).

应用多聚酶链反应-单链构像多态性分析方法分析40例喉鳞状细胞癌H-ras，K-ras，和N-ras基因的第12密码子，p53 基因第5，7外显子中点突变的情况。

After 8 weeks,PDGF-B measured with reverse transcription polymerise chain reaction and pathologic alter were observed with HE staining.

高脂喂养6周后以球囊拉伤方法建立兔颈总动脉内皮损伤模型。8周时处死动物，以逆转录聚合酶链式反应法检测动脉损伤处PDGF-B mRNA表达，HE染色观察颈动脉组织病理形态学改变。

Methods Polymerise chain reaction and restriction fragment length polymorphism were used to analyze the polymorphisms of MTHFR genes C6677T and A1298C in 56 patients of colorectal cancer and 143 age-and-sex-matched control subjects.

方法以聚合酶链反应和限制性片段长度多态方法，对56例直肠癌患者和143例正常人对照的MTHFR基因C677T和A1298C基因型进行检测分析其对直肠癌风险的相关性。结果在正常对照组中，MTHFRC677TCC、CT、TT基因型频率分别为62·90%、35·08%和2·12%，而在直肠癌病人中分别为58·93%、26·77%和14·26%。A1298C与A1298C基因型在病例和对照中的分布差异无显著性意义。

In this study,Using extracted total DNA from duck plague virus vaccine strain infected Chicken embryo fibroblasts cells as template, the 1.0 kb TK gene nonessential for viral replication was amplified by polymerise chain reaction and cloned into T-easy vector to obtain pGTK.

本研究从感染鸡胚成纤维细胞的鸭瘟病毒疫苗毒株中提取了总DNA，并以此为模板，利用PCR技术扩增出病毒生长非必需的TK基因(约1.1kb)，将其克隆入pGEM-T easy载体获得载体pGTK。

Ulcer index and serum cortisol level were used to evaluate the protective effect of acupuncturing at the acupoint ST 36; reverse transcription-polymerase chain reaction was used to detect the expression of SP and POMC in hypothalamus and adrenal, and the images were analyzed with semi-quantitative method.

胃黏膜溃疡指数和RT-PCR法研究针刺足三里对大鼠的冷应激性溃疡的保护作用和下丘脑、肾上腺的SP、POMC的表达，经图像分析系统照相并进行半定量。

Methods:Semi-quantitative reverse transcriptase polymerase chain reaction was used to examine the expression of MRP gene in 65 bone marrow aspirates of AL patients and 15 normal volunteers peripheral blood mononuclear cells.

应用半定量逆转录-聚合酶链反应，检测了65例AL患者骨髓和15名正常人外周血单个核细胞中MRP基因的表达。

Nasopharyngeal aspirates were collected from the children and assessed for 20 different respiratory pathogens using nested multiplex polymerase chain reaction, and all participants aged at least 6 years underwent exhaled nitric oxide and lung function measurements.

收集儿童鼻咽吸入物，用多重半套式PCR技术分析20种不同的呼吸道病原体，所有6岁以上受试者进行一氧化氮呼出的肺功能测试。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。