查询词典 Chain Reaction

Rats were sacrificed at 0, 6, 12, 24 and 48 hours after reproduction of pancreatitis in both groups. The intestinal mucosal permeability was assessed with 99mTechnetium Diethlene Triamine Pentacetic Acid method, and the expression of substance P was evaluated with reverse transcriptase polymerase chain reaction and Western blotting analysis.

分别于制模成功后0、6、12、24和48 h处死两组大鼠，并留取标本；利用同位素法测定肠黏膜通透性，分别用逆转录-聚合酶链反应和蛋白质免疫印迹法检测P物质在肠组织中的mRNA和蛋白表达水平。

To get more factors associated with erythropoiesis in K562 cells, modified differential display reverse transcription polymerase chain reaction method was applied to identify genes with differential expression in uninduced and hemin-induced K562 cells. Remarkable differential ESTs were firstly selected for cloning, sequencing and analyzed with bioinformatics methods.

为了进一步获得分化过程中相关基因表达的信息，我们应用改良的差异显示RT-PCR技术，分析K562细胞在Hemin诱导的红系分化前后的基因表达变化，获得红系分化相关基因的线索，进而确定在红系分化中起重要作用的基因，并研究它们的作用机制。

To get more informationon gene regulation during erythroid differentiation of K562 cells, we applied modifieddifferential display reverse transcription polymerase chain reaction methodto identify genes showing differential expression in uninduced and hemin-induced K562cells.

为进一步获得分化过程中相关基因表达的信息，我们应用改良的差异显示RT-PCR技术，分析K562细胞在Hemin诱导的红系分化前后的基因表达变化，共获得357条差异表达带。

Coli. Methods Among 95 unrepeated strains isolated from clinic, 38 positive strains tested by ESBLs confirmatory test were collected from HAI and CAI subjected to detection for drug resistant gene TEM, SHV, CTX-M-1 and for disinfectant resistant gene qacE△l-sull by polymerase chain reaction.

收集从临床标本分离的大肠埃希菌95株，经ESBLs确证试验将其中38株阳性菌株分为医院与社区获得性感染2组，用聚合酶链反应检测耐药基因TEM、SHV、CTX-M-1以及耐消毒剂基因qacE△1-su l1。

Immunofluorescence staining and reverse transcription polymerase chain reaction were used to test the expression of syndecan-1 protein and its mRNA in cultured unstimulated RPE cells.

2在正常RPE细胞，可检测到syndecan一1蛋白和mRNA的表达；第四军医大学硕士学位论文未受刺激的RPE细胞胞膜/胞浆、核仁呈现强的黄绿色荧光；随TNF一。

Objective To study the clinical significance of Ligase chain reaction in urogenital infection, we identified chlamydia trachomatis by LCR and cell culture.

目的 比较连接酶链反应与培养法检测沙眼衣原体在泌尿生殖道感染中的临床意义。

AIM: To establish a modified polymerase chain reaction method for the detection of Plasmodium vivax in the endemic areas of malaria and compare the method with the conventional light microscopy in the field.

目的： 建立适合疟疾流行区现场应用的PCR 检测间日疟的方法，并在现场与常规涂片镜检法进行比较。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Methods Polymerase chain reaction was used to detect HPV 16 DNA and point mutation at condon 12 of Ha-ras oncogene.

应用多聚酶链反应技术扩增HPV16 DNA和Ha-ras癌基因相关片段，分别采用2％琼脂糖凝胶电泳和限制性片段长度多态性分析，检测口腔癌中的HPV16 DNA和Ha-ras癌基因第12位密码子的点突变。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。