查询词典 Chain Reaction

Duck IFN-γ gene was amplified from total RNA extracted directionally from ma duck splenocyte by reverse transcription-polymerase chain reaction, then was cloned and sequenced.

应用RT-PCR技术，直接从成年麻鸭脾淋巴细胞提取的总RNA中扩增出麻鸭γ-干扰素基因，并克隆、测序。

The expression changes of ICAM-1 and p65 mRNA level were detected by reverse transcription-polymerase chain reaction and the expression of p65 protein in AR42J cell lines were detected with Stept Avidin-Biotin complex respectively, and the changes of amylase in culture medium with chromatometry.

逆转录－多聚酶链反应半定量法观察细胞间黏附分子(ICAM-1)和p65亚单位mRNA表达的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达；人工碘比色法观察培养液上清中淀粉酶的活性改变。

The expression changes of TNF-α and p65 mRNA expression were detected by reverse transcription-polymerase chain reaction, the TNF-α level in the supernatant was determined by ELISA, and the expression of p65 in AR42J cell lines was detected by Stept Avidin-Biotio Complex.

逆转录－多聚酶链反应半定量法观察肿瘤坏死因子/p65亚单位mRNA表达的变化；ELISA法检测TNF-α在细胞上清中质量浓度的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达。

By the method of polymerase chain reaction-single strand conformation polymorphism, this paper studied the genetic polymorphism of exon 1 and intron 1 in the aquaporin gene of seven species of Stipa in the meadow steppe, typical steppe, and desert steppe of Inner Mongolia.

以分布于内蒙古的草甸草原、典型草原、荒漠草原上的7种针茅属植物为材料，利用聚合酶链反应－单链构象多态性方法，分析了水孔蛋白基因的外显子1和内含子1的遗传多态性。

Suum were reported to be suitable vaccine candidates for the control of Ascaris infection. Here we cloned As37 gene by reverse transcription-polymerase chain reaction using primers based on the pubmed cDNA sequence of As37 gene.

作者已克隆了猪蛔虫rAs37基因并进行了原核表达，并通过对重组融合蛋白As37进行免疫原性分析，显示其具有良好的免疫学活性。

SP-R mRNA in nasal mucosa, obtained from two groups, were used to do reverse transcriptive polymerase chain reaction and Southern blot.

实验组用卵清蛋白行腹腔注射基础致敏，继之鼻局部激发建立大鼠变应性鼻炎模型。

IL-6 mRNA,gp80 mRNA and gp130 mRNA in freshly-isolated peripheral blood mononuclear cells from 18 active and 16 inactive LN patients,as well as 10 healthy subjects were measured by quantitative reverse transcriptive polymerase chain reaction.Results Significant levels of IL-6 mRNA were expressed in 13 (72.2%) of 18 active cases,whereas in 6 (37.5%) of 16 inactive cases and in 1 (10.0%) of 10 normal subjects.

结果 ①活动期LN患者、非活动期LN患者及正常人PBMCs IL-6 mRNA检出阳性率各为72.2%、37.5%和10.0%，前者显著高于后两者，而后两者PBMCs IL-6 mRNA的检出阳性率差异则无显著性；这三组人群PBMCs内gp80 mRNA与gp130 mRNA的检出阳性率均无统计学差异。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

Methods One hundred thirty PCOS women and one hundred seventy five normal women as controls were enrolled in this study. The genotypes were screened by polymerase chain reaction-On/off switch and the product was isolated by electrophoresis on a 2.5% agarose gel containing ethidium bromide and visualized using an ultraviolet transilluminator.

应用On/off Switch-聚合酶链反应及琼脂糖凝胶电泳分离技术分析130例多囊卵巢综合征患者及175例对照组妇女TNF-α基因rs3179060C/A多态，比较rs3179060C/A两组等位基因及基因型与PCOS及其高雄激素之间的关系。

Methods Three serologic methods including domestic-made enzyme-linked immunosorbent assay reagents, the toluidine red unheated-serum test and treponema pallidum particle agglutination test reagents were compared. The transudate fluid of the syphilis patient in genital duct was detected by polymerase chain reaction for analysis of infectivity.

采用国产酶联免疫吸附试验试剂与甲苯胺红不加热血清试验和梅毒螺旋体颗粒凝集试验进行血清学方法比较，采用聚合酶链反应对梅毒患者生殖道皮损渗出液进行检测，分析其传染性。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。